Anti-pan cytokeratin antibody

Anti-pan cytokeratin antibody

Quality level: 100

Biological source: mouse

Antibody product type: primary antibodies

Clone: AE1 / AE3, monoclonal

Species reactivity

rat, monkey, chicken, bovine, human, mouse, rabbit

Application(s)

  • ELISA: adequate
  • immunohistochemistry: adequate
  • Western blot: adequate

Isotype: IgG1

Sent in: wet ice

General description

Keratins are a group of water-insoluble proteins that form monofilaments, a class of intermediate filament. These filaments are part of the cytoskeletal complex in the epidermis and in most other epithelial tissues. Nineteen human epithelial keratins are resolved with two-dimensional gel electrophoresis (Moll et al., 1982).

These can be divided into acidic (pI <5.7) and basic (pI> 6.0) subfamilies. Acidic keratins have molecular weights of 56.5, 55, 51, 50, 50 ‘, 48, 46, 45 and 40 kDa. Basic keratins have molecular weights of 65-67, 64, 59, 58, 56, and 52 kDa. Members of the acidic and basic subfamilies are found together in pairs.

The composition of the keratin pairs varies with epithelial cell type, differentiation stage, cell growth environment, and disease state (Sun et al., 1984; Cooper et al., 1985; Sun et al., 1985); The 56.5 / 65 -67 kDa pair is present in the keratinized (differentiated) epidermis; The 55/64 kDa pair is characteristic of normal epithelial differentiation (corneal type) (Moll et al., 1982; Sun et al., 1984);

The 51/59 kDa pair is characteristic of the stratified squamous epithelium of an internal organ such as the esophagus and tongue (Moll et al., 1982; Cooper et al., 1985) The 51/58 kDa pair is a marker of keratinocytes; the pair is present in almost all stratified epithelia regardless of cell stratification status (Moll et al., 1982; Sun et al., 1984); The 48/56 kD pair is characteristic of hyperproliferative (dedifferentiated) keratinocytes (Moll et al., 1982; Weiss et al., 1984) t;

Specificity

The stringent, but broad, specificity of the combined AE1 / AE3 antibody has made this preparation very useful as a general stain for normal and neoplastic cells of epithelial origin. Anti-Keratin AE1 recognizes the 56.5, 50, 50 ‘, 48, and 40 kD keratins of the acid subfamily. Anti-keratin AE3 recognizes all members of the basic subfamily {65,67,64,59.59,56,52 human basic cytokeratins}.

Immunogen

  • Human epidermal keratins
  • Epitope: Recognizes acidic and basic cytokeratins.

Request

Anti-cytokeratin AE1 / AE3 antibody recognizes acidic and basic cytokeratins, clone AE1 / AE3 is an antibody against cytokeratin AE1 / AE3 for use in ELISA, IH, and WB.

ELISA:

An earlier batch of this antibody was used for ELISA (Woodcock-Mitchel & Sun, 1982).

Western blot:

(Woodcock-Mitchel & Sun, 1982; Tseng et al., 1982; Laster et al., 1986) ELISA: (Woodcock-Mictchel & Sun, 1982)

Immunohistochemistry (paraffin):

0.5-2 µg / ml from an earlier batch was used to stain unfixed, frozen or formalin-fixed, paraffin-embedded tissue sections (Woodcock-Mitchel & Sun, 1982; Tseng et al., 1982; Asch * Asch, 1986; Rodríguez et al., 1986; Clausen et al., 1986; Klein-Szanto et al., 1987; Reibel et al., 1985). Digestion with trypsin or pepsin is required for adequate staining in paraffin-embedded tissues. {Trypsin 1 mg / mL 10 minutes, 37 ° C or pepsin 1 mg / mL 5 minutes 37 ° C}. High temperatures with citrate antigen recovery can also be used.

Quality

Routinely evaluated by Western Blot on A431 lysates.

Western blot:

A 1: 500 dilution of this lot detected CYTOKERATIN AE1 / AE3 in 10 µg of A431 lysates.

Destination description

40-70 kDa

Physical form

  • Sodium sulfate precipitation
  • Anti-keratin AE1 / AE3 is supplied in 0.5 ml of borate buffered saline, pH 8.0, containing 0.09% sodium azide.

Format: Purified

Storage and stability

Stable for 1 year at 2-8ºC from the date of receipt. DO NOT FREEZE.

Analysis note

  • Control
  • All tissues and tumors derived from the epithelium

Other notes

Concentration – See Certificate of Analysis for lot-specific concentration.

Legal information

CHEMICAL is a registered trademark of Merck KGaA, Darmstadt, Germany

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