- Species reactivity: Human, Mouse, Rat
- Host / isotype: Rabbit / IgG
- Class: Polyclonal
- Kind: Antibody
- Immunogen: A synthesized peptide derived from human AXL (Phospho-Tyr702)
- Conjugate: Unconjugated
- Form: Liquid
- Concentration: 1 mg / mL
- Purification: Affinity chromatography
- Storage buffer: PBS, pH 7.4, with 50% glycerol
- Contains: 0.02% sodium azide
- Storage conditions: -20 ° C
- RRID: AB_2662770
The polyclonal phospho-Axl antibody (Tyr702) detects endogenous levels of Axl only when it is phosphorylated on Tyr702.
Axl is a member of the TAM receptor family, which also includes MerTK and TYRO3. Axl contains protein kinase activity and is activated by dimerization and autophosphorylation upon binding to the ligand. In response to its ligands, Protein S and GAS6, Axl activates anti-inflammatory pathways (via SOCS-1 and SOCS-3), limiting TLR and cytokine signaling. This results in buffered inflammatory responses in macrophages and developing countries.
TAM receptors also participate in the phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes related to ineffective elimination of apoptotic cells and membranes (eg, in the retina and male reproductive tract) and develop a severe autoimmune phenotype similar to systemic lupus erythematosus, including the production of broad-spectrum autoimmunity. -antibodies. Furthermore, Axl can function as a putative entry receptor for filoviruses.
Axl is also used to identify a specific subpopulation of human blood dendritic cells, also called Siglec 6 + / Axl + dendritic cells. Cellular expression of Axl can be up-regulated by TLR ligands, such as LPS or poly I: C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Elevated plasma levels can be indicative of inflammation and cancer.