Quality level: 300
Biological source: goat
Antibody form: affinity isolated antibody
Antibody product type: secondary antibodies
Species Reactivity: Rabbit
Should not react with: human
Direct ELISA: 1: 30,000 using 5 μg / ml rabbit IgG for coating and OPD substrate immunohistochemistry (paraffin-embedded sections, formalin-fixed): 1: 200 Western blot (chemiluminescent): 1: 80,000-1: 160,000 using the detection of β-actin in the total cellular extract of HeLa cells (5-10 μg / mL)
Conjugate: peroxidase conjugate
Sent in: dry ice
The goat anti-rabbit IgG peroxidase conjugate associates with all rabbit Ig. The conjugate shows no reaction with human IgG by immunoelectrophoresis (IEP). In addition, the antibody is adsorbed with human IgG to reduce background staining with human samples. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody.
An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and helps mainly in immune defense. IgG is an important class of immunoglobulin. Immunoglobulin G (IgG) is involved in type II and type III hypersensitivity. Rabbit IgG is divided into five classes: IgM, IgG, IgA, IgD, and IgE. It has only one IgG variant with an inner weighted disulfide bridge.
Specificity: The specificity of anti-rabbit IgG peroxidase is determined by immunoelectrophoresis (IEP) using normal rabbit serum and rabbit IgG. The conjugate shows no reaction with human IgG by IEP.
Immunogen: Purified rabbit IgG
Anti-rabbit IgG (whole molecule): The peroxidase antibody has been used in Western blotting, immunofluorescence staining, immunochemistry, and immunoprecipitations. Peroxidase-conjugated goat anti-rabbit IgG can be used for direct ELISA and immunohistochemistry.
Peroxidase-conjugated goat anti-rabbit IgG antibody was used for Western blot analysis of Cos 7 cell lysates at 1: 2000 dilution and yeast lysates after protein carbonylation assays at 1: dilution. 5000.
Goat-produced rabbit anti-IgG (whole molecule) -peroxidase antibody has been used as a secondary antibody during immunofluorescence staining.
Coimmunoprecipitation and Western blot analysis of C33A cell lysates was performed using HRP-conjugated goat anti-rabbit IgG as the secondary antibody.
Biochemical / Physiological Actions: IgGs (immunoglobulins) modulate various immune responses and primarily help in immune defense. Immunoglobulin G (IgG) is involved in type II and type III hypersensitivity. It aids in opsonization, complement fixation, and antibody-dependent cell-mediated cytotoxicity.
Physical Form: The solution in 0.01 M phosphate-buffered saline, pH 7.4, containing 0.05% MIT
Preparation note: Prepared by the two-step glutaraldehyde method.
Storage and stability: For continuous use, store at 2-8 ° C for up to one month. For long-term storage, the solution can be frozen in working aliquots. Repeated freezing and thawing, or storing in “frost-free” freezers is not recommended. If slight cloudiness occurs after prolonged storage, rinse the solution by centrifugation before use.
Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product (s), our products are intended for research use only and are not to be used for any other purpose, including but not limited to commercial uses. unauthorized, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption or application to humans or animals.