Functions of western blot approach: From bench to bedside
Western blot (WB) or immunoblot is a workhorse technique. It’s generally utilized by biologists for examine of various facets of protein biomolecules. As well as, it has been broadly utilized in illness prognosis. Regardless of some limitations resembling very long time, totally different functions of WB haven’t been restricted. Within the current overview, we now have summarized scientific and scientific functions of WB. As well as, we described some new era of WB strategies.
Promising proteins detected by Western blot from Echinococcus granulosus protoscoleces for predicting early post-surgical outcomes in CE-affected Tunisian kids
Background: Cystic echinococcosis (CE) impacts predominantly younger sufferers in extremely endemic areas. Improved serological strategies are wanted for the follow-up of CE circumstances, particularly given the excessive charges of post-surgical relapse that require detection as quickly as potential.
Strategies: We designed a examine to analyze the worth of antigenic proteins extracted from Echinococcus granulosus (E. granulosus) protoscoleces, and of recombinant B2t and 2B2t proteins, for assessing the efficacy of surgical remedy carried out on CE-affected kids. This examine was carried out on 278 plasma samples collected from 59 Tunisian kids surgically handled for CE and monitored for three years post-surgery.
The sufferers have been categorized in keeping with post-surgical outcomes right into a “non-relapsed” (NRCE) and a “relapsed” (RCE) group. We carried out in-house ELISAs to measure anti-B2t and anti-2B2t IgG and immunoblotting for the detection of IgG in opposition to SDS-PAGE-resolved E. granulosus protoscoleces-specific antigens. The Wilcoxon check was utilized to evaluate anti-B2t and anti-2B2t IgG ranges. We utilized the Cochran Q check to match the distribution of immunoblotting antigenic bands between 1-month and 1-year post-surgery.
Outcomes: The likelihood of being “relapse-free” when a lower in antibody titers occurred between 1 month and 1 12 months post-surgery was 81% and 75%, respectively, for anti-B2t and anti-2B2t IgG. We recognized 5 protoscolex protein bands of 20, 26/27, 30, 40 and 46 kDa as extremely immunoreactive by immunoblot for each RCE and NRCE sufferers at 1 month post-surgery, and considerably decrease immunoreactivity after 1 12 months (p < 10-4) for NRCE in comparison with RCE sufferers.
The proteins at 26/27 and 40 kDa displayed the most effective efficiency in predicting the end result, with an 84% likelihood of being relapse-free when the reactivity in opposition to the 40 kDa antigen, the doublet at 26/27 kDa, or each was absent or disappeared between 1 month and 1 12 months post-surgery, and a 93% likelihood of being relapsed when each bands remained reactive or elevated in depth between the 2 time factors.
Conclusions: The B2t protein could possibly be helpful for the prediction of CE early post-surgical outcomes. The proteins of E. granulosus protoscoleces, particularly the doublet P26/27 and P40, could possibly be promising predictive biomarkers for the post-surgical follow-up of CE circumstances as effectively.

Hypersensitive Western Blot Chemiluminescent Substrate |
|||
CR0001-25 | BosterBio | 50mL (sufficient reagents for 10000 cm2 of membrane) | EUR 314 |
Hypersensitive Western Blot Chemiluminescent Substrate |
|||
CR0001-5 | BosterBio | 10mL (sufficient reagents for 2000 cm2 of membrane) | EUR 116 |
AgileBlotter? Automated Western Blot Processor |
|||
ACT-ABH-40 | ACTGene | EUR 16939 |
Interferon alpha Western Blot Antibody |
|||
20-abx137014 | Abbexa |
|
|
Chemiluminescent Western Blot Kit (OKRA00001) |
|||
OKRA00001 | Aviva Systems Biology | 1 kit | EUR 570 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
Chemiluminescent Western Blot Kit (OKRA00002) |
|||
OKRA00002 | Aviva Systems Biology | 1 kit | EUR 570 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
Chemiluminescent Western Blot Kit (OKRA00003) |
|||
OKRA00003 | Aviva Systems Biology | 1 kit | EUR 570 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
Chemiluminescent Western Blot Kit (OKRA00004) |
|||
OKRA00004 | Aviva Systems Biology | 1 kit | EUR 570 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
Colorimetric Western Blot Kit (OKRA00014) |
|||
OKRA00014 | Aviva Systems Biology | 1 kit | EUR 609 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
Colorimetric Western Blot Kit (OKRA00015) |
|||
OKRA00015 | Aviva Systems Biology | 1 each | EUR 609 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
Colorimetric Western Blot Kit (OKRA00016) |
|||
OKRA00016 | Aviva Systems Biology | 1 kit | EUR 609 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
Colorimetric Western Blot Kit (OKRA00017) |
|||
OKRA00017 | Aviva Systems Biology | 1 kit | EUR 609 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
PDCD4 Western Blot kit (AWBK09045) |
|||
AWBK09045 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
ETS1 Western Blot kit (AWBK100604) |
|||
AWBK100604 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
ERG Western Blot kit (AWBK100625) |
|||
AWBK100625 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
GABRP Western Blot kit (AWBK13034) |
|||
AWBK13034 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
HTR1A Western Blot kit (AWBK13041) |
|||
AWBK13041 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
RAB14 Western Blot kit (AWBK13107) |
|||
AWBK13107 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
TNRC4 Western Blot kit (AWBK31525) |
|||
AWBK31525 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
ESRRG Western Blot kit (AWBK31655) |
|||
AWBK31655 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
GLI2 Western Blot kit (AWBK31885) |
|||
AWBK31885 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
PAX4 Western Blot kit (AWBK32064) |
|||
AWBK32064 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
AHR Western Blot kit (AWBK32243) |
|||
AWBK32243 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
GLI1 Western Blot kit (AWBK32368) |
|||
AWBK32368 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
PAX7 Western Blot kit (AWBK32393) |
|||
AWBK32393 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
LHX6 Western Blot kit (AWBK32553) |
|||
AWBK32553 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
HIPK2 Western Blot kit (AWBK32586) |
|||
AWBK32586 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
PAX7 Western Blot kit (AWBK32742) |
|||
AWBK32742 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
ZFP67 Western Blot kit (AWBK32749) |
|||
AWBK32749 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
OLIG2 Western Blot kit (AWBK32753) |
|||
AWBK32753 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
ENG Western Blot kit (AWBK33068) |
|||
AWBK33068 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
SOX5 Western Blot kit (AWBK33323) |
|||
AWBK33323 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
SOX10 Western Blot kit (AWBK33326) |
|||
AWBK33326 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
RPS16 Western Blot kit (AWBK33535) |
|||
AWBK33535 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
ATP7A Western Blot kit (AWBK33797) |
|||
AWBK33797 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
A1BG Western Blot kit (AWBK33810) |
|||
AWBK33810 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
NPM1 Western Blot kit (AWBK34094) |
|||
AWBK34094 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
LIG4 Western Blot kit (AWBK34122) |
|||
AWBK34122 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
RNF2 Western Blot kit (AWBK34290) |
|||
AWBK34290 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
ZFP36 Western Blot kit (AWBK34385) |
|||
AWBK34385 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
ARIH2 Western Blot kit (AWBK34418) |
|||
AWBK34418 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
KEAP1 Western Blot kit (AWBK34728) |
|||
AWBK34728 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
CLIC1 Western Blot kit (AWBK35045) |
|||
AWBK35045 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
KCNN2 Western Blot kit (AWBK35094) |
|||
AWBK35094 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
CLCN3 Western Blot kit (AWBK35504) |
|||
AWBK35504 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
P2RX7 Western Blot kit (AWBK35518) |
|||
AWBK35518 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
TRPM3 Western Blot kit (AWBK35609) |
|||
AWBK35609 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
PITX2 Western Blot kit (AWBK35634) |
|||
AWBK35634 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
ILF2 Western Blot kit (AWBK35731) |
|||
AWBK35731 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
ONECUT2 Western Blot kit (AWBK35750) |
|||
AWBK35750 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
ZFP42 Western Blot kit (AWBK36244) |
|||
AWBK36244 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
ANXA1 Western Blot kit (AWBK36568) |
|||
AWBK36568 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
SPP1 Western Blot kit (AWBK36677) |
|||
AWBK36677 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
RUNX2 Western Blot kit (AWBK36678) |
|||
AWBK36678 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
ZBP1 Western Blot kit (AWBK36711) |
|||
AWBK36711 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
Bapx1 Western Blot kit (AWBK36826) |
|||
AWBK36826 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
G3BP Western Blot kit (AWBK37713) |
|||
AWBK37713 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
TWIST1 Western Blot kit (AWBK37997) |
|||
AWBK37997 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
ID4 Western Blot kit (AWBK38058) |
|||
AWBK38058 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
SATB1 Western Blot kit (AWBK38208) |
|||
AWBK38208 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
EED Western Blot kit (AWBK38384) |
|||
AWBK38384 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
TARDBP Western Blot kit (AWBK38941) |
|||
AWBK38941 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
TARDBP Western Blot kit (AWBK38942) |
|||
AWBK38942 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
BRD7 Western Blot kit (AWBK39018) |
|||
AWBK39018 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
JMJD3 Western Blot kit (AWBK40102) |
|||
AWBK40102 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
SNRP70 Western Blot kit (AWBK40439) |
|||
AWBK40439 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
SSB Western Blot kit (AWBK40462) |
|||
AWBK40462 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
STAU1 Western Blot kit (AWBK40537) |
|||
AWBK40537 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
PCBP1 Western Blot kit (AWBK40630) |
|||
AWBK40630 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
HNRPLL Western Blot kit (AWBK41102) |
|||
AWBK41102 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
MSI2 Western Blot kit (AWBK41112) |
|||
AWBK41112 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
HNRPA3 Western Blot kit (AWBK41195) |
|||
AWBK41195 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
WNT2B Western Blot kit (AWBK41254) |
|||
AWBK41254 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
GNB1L Western Blot kit (AWBK41328) |
|||
AWBK41328 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
SLC12A1 Western Blot kit (AWBK41388) |
|||
AWBK41388 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
TPM1 Western Blot kit (AWBK41392) |
|||
AWBK41392 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
CEACAM6 Western Blot kit (AWBK41504) |
|||
AWBK41504 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
SLC22A1 Western Blot kit (AWBK41516) |
|||
AWBK41516 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
STK11 Western Blot kit (AWBK41743) |
|||
AWBK41743 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
OTC Western Blot kit (AWBK41766) |
|||
AWBK41766 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
TSHR Western Blot kit (AWBK41870) |
|||
AWBK41870 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
RHOBTB1 Western Blot kit (AWBK41883) |
|||
AWBK41883 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
KRT13 Western Blot kit (AWBK42031) |
|||
AWBK42031 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
IDH3A Western Blot kit (AWBK42237) |
|||
AWBK42237 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
SLC1A5 Western Blot kit (AWBK42247) |
|||
AWBK42247 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
PBEF1 Western Blot kit (AWBK42255) |
|||
AWBK42255 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
FST Western Blot kit (AWBK42287) |
|||
AWBK42287 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
SLC22A7 Western Blot kit (AWBK42306) |
|||
AWBK42306 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
ASPN Western Blot kit (AWBK42487) |
|||
AWBK42487 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
RSAD2 Western Blot kit (AWBK42696) |
|||
AWBK42696 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
PANX2 Western Blot kit (AWBK42778) |
|||
AWBK42778 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
PANX1 Western Blot kit (AWBK42783) |
|||
AWBK42783 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
TGFBI Western Blot kit (AWBK44268) |
|||
AWBK44268 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
PTPN2 Western Blot kit (AWBK45362) |
|||
AWBK45362 | Aviva Systems Biology | 10 reactions | EUR 647 |
Description:
|
Designing of Western Blot Method for Glanders Diagnosing in Iran
Burkholderia mallei is the etiologic agent of glanders. It’s tough to diagnose this zoonotic illness in its early levels. Some strategies such because the complement fixation check (CFT) trigger some issues for veterinary authorities and monetary losses to animal house owners as a consequence of false-positive outcomes. The mallein check requires acceptable laboratory gear and expert personnel. To shortly and precisely diagnose the illness, particularly in areas the place animals can’t be saved, new strategies (such because the Western blot check [WBT]) must be used to determine the illness.
This examine designed and optimized the Western blot (immunoblot) check utilizing sera from 84 glanderous equids, and the sensitivity and specificity of ELISA and CFT have been in contrast with the WBT. ELISA exams are based mostly on B. mallei antigens whereas a purified lipopolysaccharide-containing B. mallei antigen is used within the WBT. The sensitivity and specificity of the exams have been estimated utilizing the cut-off values really helpful by the check builders. The WBT and ELISA have been considerably extra particular than the CFT. The ELISA based mostly on B. mallei antigens was considerably much less delicate than the CFT.
Given their comparable sensitivities and specificities, the CFT (95.7%, 98.5%), the WBT (95%, 100%) and the ELISA (85%, 100%) must be additional developed. The CFT continues to be the prescribed approach for serological investigation of equids for commerce functions to certify particular person animals with out glanders. Subsequently, extra efforts must be made to additional enhance and optimize the WBT and ELISA exams.
Evaluating Host Responses to Helicobacter pylori Utilizing ELISA and Western Blot
Western blot and enzyme-linked immunosorbent assay (ELISA) are antibody-mediated strategies that are broadly used for the detection and characterization of alterations in host protein expression following H. pylori an infection . Each strategies are extremely particular and delicate for protein detection, with Western blot detection sensitivity as little as picogram quantities of the protein of curiosity, whereas the everyday ELISA detection vary is 0.01-0.1 ng. Right here we offer an experimental instance to display the applying of those strategies for the willpower of macrophage inflammatory responses following H. pylori an infection .
Western Blot as a Help Method for Immunohistochemistry to Detect Programmed Cell Loss of life Ligand 1 Expression
Antibody choice and optimization are essential to ensure correct and reproducible outcomes when utilizing such antibodies for functions resembling western blot evaluation and immunohistochemistry (IHC). That is particularly vital when deciding on good candidate antibodies that will likely be used for most cancers immunotherapy diagnostics and analysis. On this chapter, we describe a Western Blot approach as assist methodology for the choice and validation of Programmed Cell Loss of life Ligand 1 (PD-L1) antibodies that may be subsequently utilized in immunohistochemistry functions.
Western Blot is a delicate, particular, and broadly out there protein characterization approach, used for the detection of particular antigens. PD-L1 is a main immune checkpoint protein that mediates antitumor immune suppression and response, which is routinely detected utilizing IHC in formalin-fixed and paraffin-embedded tissues as a part of most cancers scientific diagnostic workflows. For that reason, it’s crucial to outline and choose the most effective antibody clones and validate them utilizing totally different strategies so as to have a dependable detection of optimistic staining when these antibodies are utilized in IHC.
Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) Antibody |
|||
abx238835-100ug | Abbexa | 100 ug | EUR 509 |
ELISA kit for Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|||
KTE101020-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2115 |
Description: Quantitative sandwich ELISA for measuring Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|||
KTE101020-48T | Abbkine | 48T | EUR 332 |
Description: Quantitative sandwich ELISA for measuring Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|||
KTE101020-96T | Abbkine | 96T | EUR 539 |
Description: Quantitative sandwich ELISA for measuring Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
Mouse Troponin C, slow skeletal and cardiac muscles, Tnnc1 ELISA |
|||
ELI-36461m | Lifescience Market | 96 Tests | EUR 865 |
Porcine Troponin C, slow skeletal and cardiac muscles, TNNC1 ELI |
|||
ELI-39993p | Lifescience Market | 96 Tests | EUR 928 |
Human Troponin C, slow skeletal and cardiac muscles, TNNC1 ELISA |
|||
ELI-40072h | Lifescience Market | 96 Tests | EUR 824 |
Bovine Troponin C, slow skeletal and cardiac muscles, TNNC1 ELIS |
|||
ELI-17222b | Lifescience Market | 96 Tests | EUR 928 |
Human Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|||
20-abx153332 | Abbexa |
|
|
Chicken Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|||
abx555347-96tests | Abbexa | 96 tests | EUR 911 |
Human Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|||
abx555435-96tests | Abbexa | 96 tests | EUR 786 |
Mouse Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|||
abx555608-96tests | Abbexa | 96 tests | EUR 739 |
Pig Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|||
abx555672-96tests | Abbexa | 96 tests | EUR 911 |
Cow Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|||
abx555769-96tests | Abbexa | 96 tests | EUR 911 |
Human TNNC1/ Troponin C, slow skeletal and cardiac muscles ELISA Kit |
|||
E2558Hu | Sunlong | 1 Kit | EUR 605 |
Human Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|||
abx352187-96tests | Abbexa | 96 tests | EUR 786 |
ELISA kit for Human Troponin C, slow skeletal and cardiac muscles |
|||
EK3670 | SAB | 96 tests | EUR 670 |
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Troponin C, slow skeletal and cardiac muscles in samples from serum, plasma, tissue homogenates and other biological fluids. |
ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|||
KTE70102-48T | Abbkine | 48T | EUR 332 |
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|||
KTE70102-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2115 |
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|||
KTE70102-96T | Abbkine | 96T | EUR 539 |
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) |
|||
KTE50026-48T | Abbkine | 48T | EUR 425 |
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) |
|||
KTE50026-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 3026 |
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) |
|||
KTE50026-96T | Abbkine | 96T | EUR 756 |
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|||
KTE30009-48T | Abbkine | 48T | EUR 354 |
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|||
KTE30009-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2252 |
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|||
KTE30009-96T | Abbkine | 96T | EUR 572 |
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol) |
|||
RAT-5 | Alpha Diagnostics | 1 | EUR 1138 |
Rat Normal Tissue Blot I |
|||
1581 | ProSci | One blot | EUR 411.5 |
Description: The rat tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The rat tissues included in the blot are brain, colon, heart, kidney, liver, lung, pancreas, and spleen. |
Rat Normal Tissue Blot II |
|||
1582 | ProSci | One blot | EUR 411.5 |
Description: The rat tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The rat tissues included in the blot are adrenal, cerebellum, cerebrum, small intestine, skeletal muscle, stomach, testis, and thymus. |
mini blotter 10x10 cm |
|||
EVS1100-BLOT | Consort | ea | EUR 682 |
mini wide blotter 20x10 cm |
|||
EVS1200-BLOT | Consort | ea | EUR 792 |
maxi blotter 20x20 cm |
|||
EVS1300-BLOT | Consort | ea | EUR 1113 |
mini blotter 10x10 cm |
|||
EVS3100-BLOT | Consort | ea | EUR 986 |
maxi blotter 20x20 cm |
|||
EVS3300-BLOT | Consort | ea | EUR 1366 |
Developmental Pluripotency Associated 4 Protein |
|||
20-abx261938 | Abbexa |
|
|
Developmental Pluripotency Associated 3 Protein |
|||
20-abx262563 | Abbexa |
|
|
Western Blot Kit |
|||
20-abx098123 | Abbexa |
|
|
IAV H6N1 (aa 17-529) (A/northern shoveler/California/HKWF115/07) [His] |
|||
DAG1686 | Creative Diagnostics | 50 ug | EUR 1681 |
Rat Interferon related developmental regulator 1(IFRD1) ELISA kit |
|||
E02I0429-192T | BlueGene | 192 tests | EUR 1270 |
Description: A competitive ELISA for quantitative measurement of Rat Interferon related developmental regulator 1(IFRD1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Interferon related developmental regulator 1(IFRD1) ELISA kit |
|||
E02I0429-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A competitive ELISA for quantitative measurement of Rat Interferon related developmental regulator 1(IFRD1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Interferon related developmental regulator 1(IFRD1) ELISA kit |
|||
E02I0429-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A competitive ELISA for quantitative measurement of Rat Interferon related developmental regulator 1(IFRD1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Developmental Pluripotency Associated 5 (DPPA5) Antibody |
|||
abx026211-400ul | Abbexa | 400 ul | EUR 523 |
Developmental Pluripotency Associated 5 (DPPA5) Antibody |
|||
abx026211-80l | Abbexa | 80 µl | EUR 286 |
Developmental Pluripotency Associated 5 (DPPA5) Antibody |
|||
20-abx338629 | Abbexa |
|
|
Total Protein Extraction Kit for Muscles (50 tests) |
|||
P5A06 | 101Bio | - | Ask for price |
Rat Skeletal Muscle Tissue Lysate |
|||
LYSATE0010 | BosterBio | 200ug | EUR 150 |
Description: This cell lysate is prepared from rat skeletal muscle tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
Rat Ceruloplasmin protein control for western blot |
|||
CP17-C | Alpha Diagnostics | 100 ul | EUR 286 |
BlotBot Mini Blot Tray |
|||
TRAY-2MINI-2 | Next Advance | 2pack | EUR 204 |
Description: Replacement trays for the BlotBot allows you to run 2 Mini sized blots simultaneaously. Pack of 2 trays. |
BlotBot Midi Blot Tray |
|||
TRAY-MIDI-2 | Next Advance | 2pack | EUR 202 |
Description: Replacement tray for the BlotBot allows you to run 1 Midi sized blot. Pack of 2 trays. |
Western Blot Enhancing Kit |
|||
K3172120 | Biochain | 1200 cm2 | EUR 143 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Western Blot Enhancing Kit |
|||
K3172250 | Biochain | 2500 cm2 | EUR 188 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Developmental Pluripotency-Associated Protein 4 (DPPA4) Antibody |
|||
20-abx301459 | Abbexa |
|
|
Interferon-Related Developmental Regulator 2 (IFRD2) Antibody |
|||
20-abx302718 | Abbexa |
|
|
Developmental Pluripotency Associated Protein 3 (DPPA3) Antibody |
|||
20-abx131612 | Abbexa |
|
|
Developmental Pluripotency Associated Protein 2 (DPPA2) Antibody |
|||
20-abx112049 | Abbexa |
|
|
Interferon-Related Developmental Regulator 1 (IFRD1) Antibody |
|||
20-abx113224 | Abbexa |
|
|
Interferon-Related Developmental Regulator 2 (IFRD2) Antibody |
|||
20-abx113225 | Abbexa |
|
|
Developmental Pluripotency-Associated Protein 4 (DPPA4) Antibody |
|||
20-abx124682 | Abbexa |
|
|
Interferon-Related Developmental Regulator 1 (IFRD1) Antibody |
|||
20-abx006953 | Abbexa |
|
|
Developmental Pluripotency Associated Protein 2 (DPPA2) Antibody |
|||
abx025285-400ul | Abbexa | 400 ul | EUR 523 |
Developmental Pluripotency Associated Protein 2 (DPPA2) Antibody |
|||
abx025285-80l | Abbexa | 80 µl | EUR 286 |
Interferon-Related Developmental Regulator 2 (IFRD2) Antibody |
|||
abx029401-400ul | Abbexa | 400 ul | EUR 523 |
Interferon-Related Developmental Regulator 2 (IFRD2) Antibody |
|||
abx029401-80l | Abbexa | 80 µl | EUR 286 |
Developmental Pluripotency Associated Protein 2 (DPPA2) Antibody |
|||
abx019070-100ug | Abbexa | 100 ug | EUR 342 |
Developmental Pluripotency Associated Protein 2 (DPPA2) Antibody |
|||
abx019071-100ug | Abbexa | 100 ug | EUR 342 |
Developmental Pluripotency-Associated Protein 4 (DPPA4) Antibody |
|||
abx028023-400ul | Abbexa | 400 ul | EUR 523 |
Developmental Pluripotency-Associated Protein 4 (DPPA4) Antibody |
|||
abx028023-80l | Abbexa | 80 µl | EUR 286 |
Interferon-Related Developmental Regulator 1 (IFRD1) Antibody |
|||
20-abx322480 | Abbexa |
|
|
Interferon-Related Developmental Regulator 2 (IFRD2) Antibody |
|||
20-abx216164 | Abbexa |
|
|
Interferon-Related Developmental Regulator 1 (IFRD1) Antibody |
|||
abx145623-100ug | Abbexa | 100 ug | EUR 391 |
Interferon-Related Developmental Regulator 1 (IFRD1) Antibody |
|||
20-abx241546 | Abbexa |
|
|
Interferon-Related Developmental Regulator 1 (IFRD1) Antibody |
|||
20-abx241547 | Abbexa |
|
|
Interferon-Related Developmental Regulator 1 (IFRD1) Antibody |
|||
abx234151-100ug | Abbexa | 100 ug | EUR 481 |
Interferon-Related Developmental Regulator 2 (IFRD2) Antibody |
|||
abx234152-100ug | Abbexa | 100 ug | EUR 551 |
Developmental Pluripotency Associated Protein 2 (DPPA2) Antibody |
|||
abx232524-100ug | Abbexa | 100 ug | EUR 551 |
Developmental Pluripotency-Associated Protein 4 (DPPA4) Antibody |
|||
abx232525-100ug | Abbexa | 100 ug | EUR 551 |
Developmental Pluripotency Associated Protein 2 (DPPA2) Antibody |
|||
20-abx210514 | Abbexa |
|
|
Developmental Pluripotency Associated Protein 2 (DPPA2) Antibody |
|||
20-abx210515 | Abbexa |
|
|
Developmental Pluripotency Associated Protein 3 (DPPA3) Antibody |
|||
20-abx334270 | Abbexa |
|
|
Developmental Pluripotency Associated 5 (DPPA5) Antibody (HRP) |
|||
20-abx335319 | Abbexa |
|
|
Developmental Pluripotency Associated 5 (DPPA5) Antibody (FITC) |
|||
20-abx335320 | Abbexa |
|
|
Developmental Pluripotency Associated 5 (DPPA5) Antibody (Biotin) |
|||
20-abx335321 | Abbexa |
|
|
Recombinant Developmental Pluripotency Associated Protein 3 (DPPA3) |
|||
4-RPC433Hu01 | Cloud-Clone |
|
|
Description: Recombinant Human Developmental Pluripotency Associated Protein 3 expressed in: E.coli |
Recombinant Developmental Pluripotency Associated Protein 3 (DPPA3) |
|||
4-RPC433Ra01 | Cloud-Clone |
|
|
Description: Recombinant Rat Developmental Pluripotency Associated Protein 3 expressed in: E.coli |
Recombinant human Developmental pluripotency-associated 5 protein |
|||
P2734 | FN Test | 100ug | Ask for price |
Description: Recombinant protein for human Developmental pluripotency-associated 5 protein |
Rat Skeletal Muscle Whole tissue lysate |
|||
RAL-1467 | Alpha Diagnostics | 1 mg | EUR 524 |
TMB Substrate, for Western Blot |
|||
F129-100 | Cygnus Technologies | 100 ml | EUR 249 |
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur. |
mini blot insert 10x10 cm |
|||
EVS1100-BLOTINSERT | Consort | ea | EUR 411 |
mini blot casette 10x10 cm |
|||
EVS1100-CASETTE | Consort | ea | EUR 65 |
miniwide blot insert 20x10 cm |
|||
EVS1200-BLOTINSERT | Consort | ea | EUR 470 |
maxi blot insert 20x20 cm |
|||
EVS1300-BLOTINSERT | Consort | ea | EUR 589 |
maxi blot casette 20x20 cm |
|||
EVS1300-CASETTE | Consort | ea | EUR 82 |
mini blot casette 10x10 cm |
|||
EVS3100-CASETTE | Consort | ea | EUR 132 |
maxi blot casette 20x20 cm |
|||
EVS3300-CASETTE | Consort | ea | EUR 158 |
TMB Substrate, for Western Blot |
|||
F100029 | Cygnus Technologies | 1000 ml | EUR 827 |
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur. |
Hypersensitive Western Blot Chemiluminescent Substrate |
|||
CR0001-10 | BosterBio | 20mL (sufficient reagents for 4000 cm2 of membrane) | EUR 167 |
Hypersensitive Western Blot Chemiluminescent Substrate |
|||
CR0001-25 | BosterBio | 50mL (sufficient reagents for 10000 cm2 of membrane) | EUR 314 |
Hypersensitive Western Blot Chemiluminescent Substrate |
|||
CR0001-5 | BosterBio | 10mL (sufficient reagents for 2000 cm2 of membrane) | EUR 116 |