Designing of Western Blot Technique for Glanders Diagnosing in Iran

Designing of Western Blot Technique for Glanders Diagnosing in Iran

Functions of western blot approach: From bench to bedside

Western blot (WB) or immunoblot is a workhorse technique. It’s generally utilized by biologists for examine of various facets of protein biomolecules. As well as, it has been broadly utilized in illness prognosis. Regardless of some limitations resembling very long time, totally different functions of WB haven’t been restricted. Within the current overview, we now have summarized scientific and scientific functions of WB. As well as, we described some new era of WB strategies.

Promising proteins detected by Western blot from Echinococcus granulosus protoscoleces for predicting early post-surgical outcomes in CE-affected Tunisian kids

Background: Cystic echinococcosis (CE) impacts predominantly younger sufferers in extremely endemic areas. Improved serological strategies are wanted for the follow-up of CE circumstances, particularly given the excessive charges of post-surgical relapse that require detection as quickly as potential.

Strategies: We designed a examine to analyze the worth of antigenic proteins extracted from Echinococcus granulosus (E. granulosus) protoscoleces, and of recombinant B2t and 2B2t proteins, for assessing the efficacy of surgical remedy carried out on CE-affected kids. This examine was carried out on 278 plasma samples collected from 59 Tunisian kids surgically handled for CE and monitored for three years post-surgery.

The sufferers have been categorized in keeping with post-surgical outcomes right into a “non-relapsed” (NRCE) and a “relapsed” (RCE) group. We carried out in-house ELISAs to measure anti-B2t and anti-2B2t IgG and immunoblotting for the detection of IgG in opposition to SDS-PAGE-resolved E. granulosus protoscoleces-specific antigens. The Wilcoxon check was utilized to evaluate anti-B2t and anti-2B2t IgG ranges. We utilized the Cochran Q check to match the distribution of immunoblotting antigenic bands between 1-month and 1-year post-surgery.

Outcomes: The likelihood of being “relapse-free” when a lower in antibody titers occurred between 1 month and 1 12 months post-surgery was 81% and 75%, respectively, for anti-B2t and anti-2B2t IgG. We recognized 5 protoscolex protein bands of 20, 26/27, 30, 40 and 46 kDa as extremely immunoreactive by immunoblot for each RCE and NRCE sufferers at 1 month post-surgery, and considerably decrease immunoreactivity after 1 12 months (p < 10-4) for NRCE in comparison with RCE sufferers.

The proteins at 26/27 and 40 kDa displayed the most effective efficiency in predicting the end result, with an 84% likelihood of being relapse-free when the reactivity in opposition to the 40 kDa antigen, the doublet at 26/27 kDa, or each was absent or disappeared between 1 month and 1 12 months post-surgery, and a 93% likelihood of being relapsed when each bands remained reactive or elevated in depth between the 2 time factors.

Conclusions: The B2t protein could possibly be helpful for the prediction of CE early post-surgical outcomes. The proteins of E. granulosus protoscoleces, particularly the doublet P26/27 and P40, could possibly be promising predictive biomarkers for the post-surgical follow-up of CE circumstances as effectively.

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RW-306-D 1 Blot
EUR 789

Rat Mammary Gland Developmental Western Blot

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Rat Skeletal Muscles Developmental Western Blot

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Mouse Brain Developmental Western Blot

MW-201-D 1 Blot
EUR 789

Mouse Ovary Developmental Western Blot

MW-406-D 1 Blot
EUR 668

Mouse Testis Developmental Western Blot

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Mouse Intestine Developmental Western Blot

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EUR 789

Rat Heart Developmental Northern Blot

RN-801-D 1 Blot
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Mouse Mammary Gland Developmental Western Blot

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Mouse Skeletal Muscles Developmental Western Blot

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EUR 789

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Rat Mammary Gland Developmental Northern Blot

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Mouse Brain Developmental Northern Blot

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Mouse Ovary Developmental Northern Blot

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Mouse Testis Developmental Northern Blot

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Limb Bud And Heart Development (LBH) Antibody

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Limb Bud And Heart Development (LBH) Antibody

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Limb Bud And Heart Development (LBH) Antibody

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Rat Brain Developmental Total Protein Panel, any 6 stages

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Limb Bud And Heart Development (LBH) Antibody (HRP)

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Limb Bud And Heart Development (LBH) Antibody (HRP)

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Limb Bud And Heart Development (LBH) Antibody (FITC)

abx337277-100g 100 µg
EUR 362.5

Limb Bud And Heart Development (LBH) Antibody (FITC)

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Breast various pathology developmental process tissue array

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Developmental Pluripotency Associated 5 (DPPA5) Antibody (HRP)

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Developmental Pluripotency Associated 5 (DPPA5) Antibody (FITC)

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Developmental Pluripotency Associated 5 (DPPA5) Antibody (FITC)

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Developmental Pluripotency Associated 5 (DPPA5) Antibody (FITC)

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Developmental Pluripotency Associated 5 (DPPA5) Antibody (FITC)

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Human Developmental Endothelial locus 1, DEL-1 GENLISA ELISA

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LBH (GFP-tagged) - Human limb bud and heart development homolog (mouse) (LBH)

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Interferon-Related Developmental Regulator 1 (IFRD1) Antibody

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Developmental Pluripotency Associated 5 (DPPA5) Antibody (Biotin)

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Interferon-Related Developmental Regulator 2 (IFRD2) Antibody

20-abx302718
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Developmental Pluripotency Associated Protein 3 (DPPA3) Antibody

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Developmental Pluripotency Associated Protein 2 (DPPA2) Antibody

20-abx210514
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Developmental Pluripotency Associated Protein 2 (DPPA2) Antibody

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Designing of Western Blot Method for Glanders Diagnosing in Iran

Burkholderia mallei is the etiologic agent of glanders. It’s tough to diagnose this zoonotic illness in its early levels. Some strategies such because the complement fixation check (CFT) trigger some issues for veterinary authorities and monetary losses to animal house owners as a consequence of false-positive outcomes. The mallein check requires acceptable laboratory gear and expert personnel. To shortly and precisely diagnose the illness, particularly in areas the place animals can’t be saved, new strategies (such because the Western blot check [WBT]) must be used to determine the illness.

This examine designed and optimized the Western blot (immunoblot) check utilizing sera from 84 glanderous equids, and the sensitivity and specificity of ELISA and CFT have been in contrast with the WBT. ELISA exams are based mostly on B. mallei antigens whereas a purified lipopolysaccharide-containing B. mallei antigen is used within the WBT. The sensitivity and specificity of the exams have been estimated utilizing the cut-off values really helpful by the check builders. The WBT and ELISA have been considerably extra particular than the CFT. The ELISA based mostly on B. mallei antigens was considerably much less delicate than the CFT.

Given their comparable sensitivities and specificities, the CFT (95.7%, 98.5%), the WBT (95%, 100%) and the ELISA (85%, 100%) must be additional developed. The CFT continues to be the prescribed approach for serological investigation of equids for commerce functions to certify particular person animals with out glanders. Subsequently, extra efforts must be made to additional enhance and optimize the WBT and ELISA exams.

Evaluating Host Responses to Helicobacter pylori Utilizing ELISA and Western Blot

Western blot and enzyme-linked immunosorbent assay (ELISA) are antibody-mediated strategies that are broadly used for the detection and characterization of alterations in host protein expression following H. pylori an infection . Each strategies are extremely particular and delicate for protein detection, with Western blot detection sensitivity as little as picogram quantities of the protein of curiosity, whereas the everyday ELISA detection vary is 0.01-0.1 ng. Right here we offer an experimental instance to display the applying of those strategies for the willpower of macrophage inflammatory responses following H. pylori an infection .

Western Blot as a Help Method for Immunohistochemistry to Detect Programmed Cell Loss of life Ligand 1 Expression

Antibody choice and optimization are essential to ensure correct and reproducible outcomes when utilizing such antibodies for functions resembling western blot evaluation and immunohistochemistry (IHC). That is particularly vital when deciding on good candidate antibodies that will likely be used for most cancers immunotherapy diagnostics and analysis. On this chapter, we describe a Western Blot approach as assist methodology for the choice and validation of Programmed Cell Loss of life Ligand 1 (PD-L1) antibodies that may be subsequently utilized in immunohistochemistry functions.

Western Blot is a delicate, particular, and broadly out there protein characterization approach, used for the detection of particular antigens. PD-L1 is a main immune checkpoint protein that mediates antitumor immune suppression and response, which is routinely detected utilizing IHC in formalin-fixed and paraffin-embedded tissues as a part of most cancers scientific diagnostic workflows. For that reason, it’s crucial to outline and choose the most effective antibody clones and validate them utilizing totally different strategies so as to have a dependable detection of optimistic staining when these antibodies are utilized in IHC.

 

Mouse Skeletal Muscles Developmental Western Blot

MW-102-D 1 Blot
EUR 789

Rat Brain Developmental Northern Blot

RN-201-D 1 Blot
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Rat Ovary Developmental Northern Blot

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Rat Heart Developmental Northern Blot

RN-801-D 1 Blot
EUR 789

Rat Testis Developmental Northern Blot

RN-401-D 1 Blot
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Mouse Skeletal Muscles Developmental NB

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Rat Mammary Gland Developmental Northern Blot

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EUR 910

Mouse Brain Developmental Northern Blot

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Mouse Ovary Developmental Northern Blot

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EUR 789

Mouse Heart Developmental Northern Blot

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EUR 789

Mouse Testis Developmental Northern Blot

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EUR 789

Rat Skeletal Muscles Developmental Total Protein Panel, 6 stages

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EUR 998

Rat Skeletal Muscles Developmental Total Protein Panel, any 10 stages

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EUR 1289

Rat Brain Developmental Western Blot

RW-201-D 1 Blot
EUR 789

Rat Ovary Developmental Western Blot

RW-406-D 1 Blot
EUR 668

Rat Heart Developmental Western Blot

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EUR 789

Rat Testis Developmental Western Blot

RW-401-D 1 Blot
EUR 668

Rat Intestine Developmental Western Blot

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Rat Intestine developmental Western Blot

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EUR 789

Rat Mammary Gland Developmental Western Blot

RW-414-D 1 Blot
EUR 910

Mouse Brain Developmental Western Blot

MW-201-D 1 Blot
EUR 789

Mouse Ovary Developmental Western Blot

MW-406-D 1 Blot
EUR 668

Mouse Heart Developmental Western Blot

MW-801-D 1 Blot
EUR 789

Mouse Testis Developmental Western Blot

MW-401-D 1 Blot
EUR 668

Mouse Intestine Developmental Western Blot

MW-306-D 1 Blot
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Mouse Mammary Gland Developmental Western Blot

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EUR 910

Rat Skeletal Muscles cDNA

RD-102 30 reactions
EUR 243

Rat Skeletal Muscles Total RNA

RR-102 0.05mg
EUR 160

Rat Skeletal Muscles Genomic DNA

RG-102 0.1mg
EUR 177

Rat Skeletal Muscles Total Protein

RT-102 1mg
EUR 153

Rat Skeletal Muscles Frozen Sections

RF-102 10 slides
EUR 228

Rat WS Skeletal Muscles Total RNA

RR-102-WS 0.05mg
EUR 160

Rat Skeletal Muscles Paraffin Sections

RP-102 10 slides
EUR 228

Dog Skeletal Muscles RNA

DR-102 0.05mg
EUR 195

Rat WS Skeletal Muscles Total Protein

RT-102-WS 1mg
EUR 153

Dog Skeletal Muscles cDNA

DD-102 30 Reactions
EUR 280

Cat Skeletal Muscles cDNA

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Pig Skeletal Muscles cDNA

PD-102 30 reactions
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Rat WS Skeletal Muscles Frozen Sections

RF-102-WS 10 slides
EUR 228

Sheep Skeletal Muscles cDNA

SD-102 30 reactions
EUR 243

Equine Skeletal Muscles cDNA

ED-102 30 reactions
EUR 319

Bovine Skeletal Muscles cDNA

BD-102 30 reactions
EUR 243

Rabbit Skeletal Muscles cDNA

TD-102 30 reactions
EUR 243

Rat WS Skeletal Muscles Paraffin Sections

RP-102-WS 10 slides
EUR 228

Rat Skeletal Muscles cDNA-Random Primer

RD-102-RH 30 reactions
EUR 243

Chicken Skeletal Muscles cDNA

CD-102 30 reactions
EUR 243

Hamster Skeletal Muscles cDNA

AD-102 30 reactions
EUR 243

MiniPig Skeletal Muscles cDNA

ND-102 30 reactions
EUR 358

Rat WS Skeletal Muscles cDNA-Oligo-dT

RD-102-WS 30 reactions
EUR 243

Rat Placenta Premade Northern Blot

RN-413 1 Blot
EUR 668

Chicken Skeletal Muscles Sections

CP-102 10 slides
EUR 240

Cat Skeletal Muscles Total RNA

FR-102 0.05mg
EUR 195

Mouse CD1 Skeletal Muscles cDNA

MD-102 30 reactions
EUR 243

Mouse BLC Skeletal Muscles cDNA

MD-102-BLC 30 reactions
EUR 280

Mouse C57 Skeletal Muscles cDNA

MD-102-C57 30 reactions
EUR 280

Dog Skeletal Muscles Genomic DNA

DG-102 0.1mg
EUR 210

Human Skeletal Muscles Total RNA

HR-102 0.025mg
EUR 172

Guinea Pig Skeletal Muscles cDNA

GD-102 30 reactions
EUR 243

Cat Skeletal Muscles Genomic DNA

FG-102 0.1mg
EUR 210

Pig Skeletal Muscles Genomic DNA

PG-102 0.1mg
EUR 177

Sheep Skeletal Muscles Total RNA

SR-102 0.05mg
EUR 160

Mouse Intestine developmental NB

MN-306-D 1 Blot
EUR 789

Bovine Skeletal Muscles Total RNA

BR-102 0.05mg
EUR 160

Equine Skeletal Muscles Total RNA

ER-102 0.05mg
EUR 195

Rabbit Skeletal Muscles Total RNA

TR-102 0.05mg
EUR 160

Dog Skeletal Muscles Total Protein

DT-102 1mg
EUR 176

Hamster Skeletal Muscles Total RNA

AR-102 0.05mg
EUR 160

Chicken Skeletal Muscles Total RNA

CR-102 0.05mg
EUR 160

Human Skeletal Muscles Genomic DNA

HG-102 0.05mg
EUR 210

Cat Skeletal Muscles Total Protein

FT-102 1mg
EUR 176

Pig Skeletal Muscles Total Protein

PT-102 1mg
EUR 153

MiniPig Skeletal Muscles Total RNA

NR-102 0.05mg
EUR 231

Mouse Skeletal Muscles Genomic DNA

MG-102 0.1mg
EUR 177

Sheep Skeletal Muscles Genomic DNA

SG-102 0.1mg
EUR 177

Bovine Skeletal Muscles Genomic DNA

BG-102 0.1mg
EUR 177

Equine Skeletal Muscles Genomic DNA

GE-102 0.1mg
EUR 210

Rabbit Skeletal Muscles Genomic DNA

TG-102 0.1mg
EUR 177

Monkey Skeletal Muscles cDNA, Rhesus

UD-102 30 reactions
EUR 316

Dog Skeletal Muscles Frozen Sections

DF-102 10 slides
EUR 261

Human Skeletal Muscles Total Protein

HT-102 1mg
EUR 176

Hamster Skeletal Muscles Genomic DNA

GA-102 0.1mg
EUR 177

Cat Skeletal Muscles Frozen Sections

FF-102 10 slides
EUR 261

Pig Skeletal Muscles Frozen Sections

PF-102 10 slides
EUR 261

Chicken Skeletal Muscles Genomic DNA

GC-102 0.1mg
EUR 177

Sheep Skeletal Muscles Total Protein

ST-102 1mg
EUR 153

Rat Whole Embryo Premade Northern Blot

RN-104 1 Blot
EUR 668

Bovine Skeletal Muscles Total Protein

BT-102 1mg
EUR 153

Equine Skeletal Muscles Total protein

ET-102 1mg
EUR 176

Rabbit Skeletal Muscles Total Protein

TT-102 1mg
EUR 153

Rat Mammary Gland Premade Northern Blot

RN-414 1 Blot
EUR 668

Mouse Mammary Gland Developmental NB

MN-414-D 1 Blot
EUR 910

Dog Skeletal Muscles paraffin Sections

DP-102 10 slides
EUR 240

Chicken Skeletal Muscles Total Protein

CT-102 1mg
EUR 153

Hamster Skeletal Muscles Total Protein

AT-102 1mg
EUR 153

Human Skeletal Muscles Frozen Sections

HF-102 10slides
EUR 240

Cat Skeletal Muscles Paraffin Sections

FP-102 10 slides
EUR 240

MiniPig Skeletal Muscles Total Protein

NT-102 1mg
EUR 176

Sheep Skeletal Muscles Frozen Sections

SF-102 10 slides
EUR 261

Equine Skeletal Muscles Frozen Sections

EF-102 10 slides
EUR 261

Bovine Skeletal Muscles Frozen Sections

BF-102 10 slides
EUR 261

Rabbit Skeletal Muscles Frozen Sections

TF-102 10 slides
EUR 240

Mouse CD1 Skeletal Muscles Total RNA

MR-102 0.05mg
EUR 160

Mouse C57 Skeletal Muscles Total RNA

MR-102-C57 0.05mg
EUR 180

Chicken Skeletal Muscles Frozen Sections

CF-102 10 slides
EUR 261

Hamster Skeletal Muscles Frozen Sections

AF-102 10 slides
EUR 240

Human Skeletal Muscles Paraffin Sections

HP-102 10 slides
EUR 228

MiniPig Skeletal Muscles Frozen Sections

NF-102 10 slides
EUR 307

Sheep Skeletal Muscles Paraffin Sections

SP-102 10 slides
EUR 240

Guinea Pig Skeletal Muscles Total RNA

GR-102 0.05mg
EUR 160

Mini Pig Skeletal Muscles Genomic DNA

GN-102 0.1mg
EUR 210

Bovine Skeletal Muscles Paraffin Sections

BP-102 10 slides
EUR 240

Mouse Balbc Skeletal Muscles Total RNA

MR-102-BLC 0.05mg
EUR 180

Mouse C57 Skeletal Muscles Genomic DNA

MG-102-C57 0.025mg
EUR 210

Hamster Skeletal Muscles Paraffin Sections

AP-102 10 slides
EUR 228

MiniPig Skeletal Muscles Paraffin Sections

NP-102 10 slides
EUR 307

Rat Skin Developmental Total Protein Panel, any 6 stages

RT-101-006D 6x0.1mg
EUR 998

Rat Skin Developmental Total Protein Panel, any 10 stages

RT-101-010D 10x0.1mg
EUR 1289

Guinea Pig Skeletal Muscles Genomic DNA

GG-102 0.1mg
EUR 177

Rat Brain Developmental Total Protein Panel, any 6 stages

RT-201-006D 6x0.1mg
EUR 998

Rat Brain Developmental Total Protein Panel, any 10 stages

RT-201-010D 10x0.1mg
EUR 1289

Rat Liver Developmental Total Protein Panel, any 6 stages

RT-314-006D 6x0.1mg
EUR 998

Rat Liver Developmental Total Protein Panel, any 10 stages

RT-314-010D 10x0.1mg
EUR 1289

Rat Ovary Developmental Total Protein Panel, any 6 stages

RT-406-006D 6x0.1mg
EUR 1289

Rat Heart Developmental Total Protein Panel, any 6 stages

RT-801-006D 6x0.1mg
EUR 998

Rat Heart Developmental Total Protein Panel, any 10 stages

RT-801-010D 10x0.1mg
EUR 1289

Human Skeletal Muscles cDNA-Oligo-dT

HD-102 30 reactions
EUR 280

Mouse CD1 Skeletal Muscles Total Protein

MT-102 1mg
EUR 153

Mouse BLC Skeletal Muscles Total Protein

MT-102-BLC 1mg
EUR 180

Mouse C57 Skeletal Muscles Total Protein

MT-102-C57 1mg
EUR 180

Monkey Skeletal Muscles Total RNA, Rhesus

UR-102 0.05mg
EUR 195

Rat Testis Developmental Total Protein Panel, any 6 stages

RT-401-006D 6x0.1mg
EUR 1289

Guinea Pig Skeletal Muscles Total Protein

GT-102 1mg
EUR 153

Rat Stomach Developmental Total Protein Panel, any 6 stages

RT-302-006D 6x0.1mg
EUR 998

Rat Stomach Developmental Total Protein Panel, any 10 stages

RT-302-010D 10x0.1mg
EUR 1289

Mouse CD1 Skeletal Muscles Frozen Sections

MF-102 10 slides
EUR 228

Mouse BLC Skeletal Muscles Frozen Sections

MF-102-BLC 10 slides
EUR 253

Mouse C57 Skeletal Muscles Frozen Sections

MF-102-C57 10 slides
EUR 253

Monkey Rhesus Skeletal Muscles Genomic DNA

UG-102 0.1mg
EUR 210

Human Skeletal Muscles cDNA-Random Primer

HD-102-HR 30 reactions
EUR 280

Guinea Pig Skeletal Muscles Frozen Sections

GF-102 10 slides
EUR 240

Dog Tissue Premade Northern Blot

DN-MT-1 1 Blot
EUR 1153

Monkey Cyno Tissue Northern Blot

KN-MT-1 1 Blot
EUR 1153

Pig Tissue Premade Northern Blot

PN-MT-1 1 Blot
EUR 1031

Monkey Skeletal Muscles Total RNA, Cynomolgus

KR-102 0.05mg
EUR 195

Mouse CD1 Skeletal Muscles Paraffin Sections

MP-102 10 slides
EUR 228

Mouse BLC Skeletal Muscles Paraffin Sections

MP-102-BLC 10 slides
EUR 253

Mouse C57 Skeletal Muscles Paraffin Sections

MP-102-C57 10 slides
EUR 253

Monkey Skeletal Muscles Total Protein, Rhesus

UT-102 1mg
EUR 176

Rat Intestine Developmental Total Protein Panel, any 6 stages

RT-306-006D 6x0.1mg
EUR 998

Rat Intestine Developmental Total Protein Panel, any 10 stages

RT-306-010D 10x0.1mg
EUR 1289

Guinea Pig Skeletal Muscles Paraffin Sections

GP-102 10 slides
EUR 228

Human Tissue Premade Northern Blot

HN-MT-1 1 Blot
EUR 1153

Plant Tissue Premade Northern Blot

LN-MT-1 1 Blot
EUR 1335

Sheep Tissue Premade Northern Blot

SN-MT-1 1 Blot
EUR 1031

Rat Lung Developmental Total Protein Panel, set of any 6 stages

RT-601-006D 6x0.1mg
EUR 998

Rat Lung Developmental Total Protein Panel, set of any 10 stages

RT-601-010D 10x0.1mg
EUR 1289

Monkey Cynomolgus Skeletal Muscles Genomic DNA

KG-102 0.1mg
EUR 210

Bovine Tissue Premade Northern Blot

BN-MT-1 1 Blot
EUR 1031

Equine Tissue Premade Northern Blot

EN-MT-1 1 Blot
EUR 1153

Rabbit Tissue Premade Northern Blot

TN-MT-1 1 Blot
EUR 1031

Hamster Tissue Premade Northern Blot

AN-MT-1 1 Blot
EUR 1031

Chicken Tissue Premade Northern Blot

CN-MT-1 1 Blot
EUR 1031

Minipig Tissue Premade Northern Blot

NN-MT-1 1 blot
EUR 1153

Developmental Pluripotency Associated 4 Protein

20-abx261938
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  • 1 mg
  • 20 ug
  • 5 ug

Developmental Pluripotency Associated 3 Protein

20-abx262563
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  • 10 ug
  • 1 mg
  • 2 µg

Developmental Pluripotency Associated 4 Protein

abx261938-10mg 10 mg
EUR 4625

Developmental Pluripotency Associated 4 Protein

abx261938-5mg 5 mg
EUR 325

Developmental Pluripotency Associated 3 Protein

abx262563-10mg 10 mg
EUR 325

Developmental Pluripotency Associated 3 Protein

abx262563-25mg 25 mg
EUR 6575

Developmental Pluripotency Associated 3 Protein

abx262563-5mg 5 mg
EUR 225

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