Identification of early diagnostic antigens in soluble proteins of Trichinella spiralis

Identification of early diagnostic antigens in soluble proteins of Trichinella spiralis

Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Mitochondrial Respiratory Chain Complexes

Cardiac, neuronal and renal tubular epithelial cells are the most metabolically active cells in the body. Their fate depends largely on their mitochondria as the primary energy generating system which participates in the control of apoptosis, cell cycle and metabolism. Thus, mitochondrial dysfunction is a hallmark of many chronic diseases including diabetic nephropathy. A drop in mitochondrial bioenergetics efficiency is often associated with altered expression of respiratory chain complexes. Moreover, recent studies demonstrate that cellular proteins can shuttle to mitochondria and modify their function directly.

Here we illustrate two mitochondria isolation protocols; one is recommended if the purity of the mitochondrial fraction is a priority such as if the mitochondrial localization of a protein has to be validated, the other if a high yield of intact functional mitochondria is required for functional studies and quantitative Western blotting. Next, we provide a detailed protocol for Western blotting of isolated mitochondria and renal cortex either to prove the purity of isolated fractions or to quantify complexes of the mitochondrial respiratory chain. We used this approach to identify classically cell membrane bound angiotensin II receptors in mitochondria and to study the effect of these receptors on mitochondrial function in early stages of diabetic nephropathy.

Identification of early diagnostic antigens in soluble proteins of Trichinella spiralis adult worms by Western blot

Previous studies showed that crude antigens from Trichinella spiralis adult worms (AW) can be recognized by mouse infection sera at 8 days post infection. The aim of this study was to identify the early diagnostic antigenic bands in soluble proteins from T. spiralis AW by Western blot using early infection sera. The affecting factors of adult recovery were firstly observed in this study, and the results showed that the maximum number of adults was collected from small intestine when the female BALB/c mice were orally infected with 4000 ML and sacrificed at 3 days post infection.

The results of Western blot analysis showed that seven protein bands (31, 35.1, 39, 40.6, 41.9, 47 and 50.6 kDa) could be recognized by early infection sera as early as at 8-10 days post infection, and were strongly reacted with mouse infection sera at 11-12 days post infection. Our results suggested that the seven protein bands of T. spiralis AW soluble proteins might be the early expressed antigens during the intestinal stage of Trichinella infection and therefore have potential value for the early diagnosis of trichinellosis.

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Hamster Tissue Custom Made Western Blot-1

AW-CUS-1 1 Blot
EUR 668

Minipig Tissue Custom Made Western Blot-1

NW-CUS-1 1 Blot
EUR 789

Dog Tissue Custom Made Western Blot-2

DW-CUS-2 1 Blot
EUR 910

Pig Tissue Custom Made Western Blot-2

PW-CUS-2 1 Blot
EUR 789

Plant Tissue Custom Made Western Blot-2

LW-CUS-2 1 Blot
EUR 1086

Human Tissue Custom Made Western Blot-2

HW-CUS-2 1 Blot
EUR 1031

Sheep Tissue Custom Made Western Blot-2

SW-CUS-2 1 Blot
EUR 789

Bovine Tissue Custom Made Western Blot-2

BW-CUS-2 1 Blot
EUR 789

Rabbit Tissue Custom Made Western Blot-2

TW-CUS-2 1 Blot
EUR 789

Chicken Tissue Custom Made Western Blot-2

CW-CUS-2 1 Blot
EUR 789

Hamster Tissue Custom Made Western Blot-2

AW-CUS-2 1 Blot
EUR 789

Minipig Tissue Custom Made Western Blot-2

NW-CUS-2 1 Blot
EUR 910

Guinea Pig Tissue Custom Made Western Blot-1

GW-CUS-1 1 Blot
EUR 668

Monkey Cyno Tissue Custom Made Western Blot-1

KW-CUS-1 1 Blot
EUR 789

Monkey Rhesus Tissue Custom Made Western Blot-1

UW-CUS-1 1 Blot
EUR 789

Panel 7: Rat Tissue Custom Made Western Blot-1

RW-CUS-1 1 Blot
EUR 722

Guinea Pig Tissue Custom Made Western Blot-2

GW-CUS-2 1 Blot
EUR 789

Monkey Cyno Tissue Custom Made Western Blot-2

KW-CUS-2 1 Blot
EUR 910

Monkey Rhesus Tissue Custom Made Western Blot-2

UW-CUS-2 1 Blot
EUR 910

Panel 8: Rat Tissue Custom Made Western Blot-2

RW-CUS-2 1 Blot
EUR 843

Panel 9: Rat Tissue Custom Made Western Blot-3

RW-CUS-3 1 Blot
EUR 965

Panel 7: Mouse CD1 Tissue Custom Made Western Blot-1

MW-CUS-1 1 Blot
EUR 722

Panel 7: Mouse C57 Tissue Custom Made Western Blot-1

MW-CUS-1-C57 1 Blot
EUR 843

Panel 8: Mouse CD1 Tissue Custom Made Western Blot-2

MW-CUS-2 1 Blot
EUR 843

Panel 8: Mouse C57 Tissue Custom Made Western Blot-2

MW-CUS-2-C57 1 Blot
EUR 965

Panel 9: Mouse CD1 Tissue Custom Made Western Blot-3

MW-CUS-3 1 Blot
EUR 965

Panel 9: Mouse C57 Tissue Custom Made Western Blot-3

MW-CUS-3-C57 1 Blot
EUR 1086

Equine Tissue Custom Made NB-1

EN-CUS-1 1 Blot
EUR 910

Equine Tissue Custom Made NB-2

EN-CUS-2 1 Blot
EUR 1031

Dog Tissue Custom Made Northern Blot-1

DN-CUS-1 1 Blot
EUR 910

Equine Tissue Premade Western Blot

EW-MT-1 1 Blot
EUR 1031

Plant Tissue Custom Made Northern Blot-1

LN-CUS-1 1 Blot
EUR 910

Dog Tissue Custom Made Northern Blot-2

DN-CUS-2 1 Blot
EUR 1031

Plant Tissue Custom Made Northern Blot-2

LN-CUS-2 1 Blot
EUR 1153

Panel 7: Rat Tissue Custom Made Northern Blot-1

RN-CUS-1 1 Blot
EUR 722

Panel 8: Rat Tissue Custom Made Northern Blot-2

RN-CUS-2 1 Blot
EUR 843

Panel 9: Rat Tissue Custom Made Northern Blot-3

RN-CUS-3 1 Blot
EUR 965

Panel 7: Mouse CD1 Tissue Custom Made Northern Blot-1

MN-CUS-1 1 Blot
EUR 722

Panel 7: Mouse C57 Tissue Custom Made Northern Blot-1

MN-CUS-1-C57 1 Blot
EUR 910

Panel 8: Mouse CD1 Tissue Custom Made Northern Blot-2

MN-CUS-2 1 Blot
EUR 843

Panel 8: Mouse C57 Tissue Custom Made Northern Blot-2

MN-CUS-2-C57 1 Blot
EUR 1031

Panel 9: Mouse CD1 Tissue Custom Made Northern Blot-3

MN-CUS-3 1 Blot
EUR 965

Panel 9: Mouse C57 Tissue Custom Made Northern Blot-3

MN-CUS-3-C57 1 Blot
EUR 1153

Pig Tissue Custom Made NB-1

PN-CUS-1 1 Blot
EUR 789

Sheep Tissue Custom Made NB-1

SN-CUS-1 1 Blot
EUR 789

Human Tissue Custom Made NB-1

HN-CUS-1 1 Blot
EUR 910

Bovine Tissue Custom Made NB-1

BN-CUS-1 1 Blot
EUR 789

Rabbit Tissue Custom Made NB-1

TN-CUS-1 1 blot
EUR 789

Hamster Tissue Custom Made NB-1

AN-CUS-1 1 Blot
EUR 789

Chicken Tissue Custom Made NB-1

CN-CUS-1 1 blot
EUR 789

Minipig Tissue Custom Made NB-1

NN-CUS-1 1 Blot
EUR 910

Pig Tissue Custom Made NB-2

PN-CUS-2 1 Blot
EUR 910

Guinea Pig Tissue Custom Made NB-1

GN-CUS-1 1 Blot
EUR 789

Sheep Tissue Custom Made NB-2

SN-CUS-2 1 Blot
EUR 910

Human Tissue Custom Made NB-2

HN-CUS-2 1 Blot
EUR 1031

Monkey Cyno Tissue Custom Made NB-1

KN-CUS-1 1 Blot
EUR 910

Bovine Tissue Custom Made NB-2

BN-CUS-2 1 Blot
EUR 910

Rabbit Tissue Custom Made NB-2

TN-CUS-2 1 Blot
EUR 910

Hamster Tissue Custom Made NB-2

AN-CUS-2 1 Blot
EUR 910

Chicken Tissue Custom Made NB-2

CN-CUS-2 1 blot
EUR 910

Minipig Tissue Custom Made NB-2

NN-CUS-2 1 Blot
EUR 1031

Monkey Rhesus Tissue Custom Made NB-1

UN-CUS-1 1 Blot
EUR 910

Guinea Pig Tissue Custom Made NB-2

GN-CUS-2 1 Blot
EUR 910

Monkey Cyno Tissue Custom Made NB-2

KN-CUS-2 1 Blot
EUR 1031

Monkey Rhesus Tissue Custom Made NB-2

UN-CUS-2 1 Blot
EUR 1031

Equine Tissue Premade Northern Blot

EN-MT-1 1 Blot
EUR 1153

Dog Tissue Premade Western Blot

DW-MT-1 1 Blot
EUR 1031

Pig Tissue Premade Western Blot

PW-MT-1 1 Blot
EUR 910

Pig Tissue Premade Western Blot

PW-MT-1-1 0.1mg
EUR 910

Plant Tissue Premade Western Blot

LW-MT-1 1 Blot
EUR 1208

Human Tissue Premade Western Blot

HW-MT-1 1 Blot
EUR 1153

Sheep Tissue Premade Western Blot

SW-MT-1 1 Blot
EUR 910

Bovine Tissue Premade Western Blot

BW-MT-1 1 Blot
EUR 910

Rabbit Tissue Premade Western Blot

TW-MT-1 1 Blot
EUR 910

Chicken Tissue Premade Western Blot

CW-MT-1 1 Blot
EUR 910

Hamster Tissue Premade Western Blot

AW-MT-1 1 Blot
EUR 910

Minipig Tissue Premade Western Blot

NW-MT-1 1 Blot
EUR 1031

Guinea Pig Tissue Premade Western Blot

GW-MT-1 1 Blot
EUR 910

Monkey Cyo Tissue Premade Western Blot

KW-MT-1 1 Blot
EUR 1031

Monkey Cyo Tissue Premade Western Blot

KW-MT-1-1 1 Blot
EUR 1031

Monkey Rhesus Tissue Premade Western Blot

UW-MT-1 1 Blot
EUR 1031

Panel 1: Rat Major Tissue Premade Western Blot

RW-MT-1 1 Blot
EUR 965

Panel 1: Mouse CD1 Major Tissue Premade Western Blot

MW-MT-1 1 Blot
EUR 965

Panel 1: Mouse C57 Major Tissue Premade Western Blot

MW-MT-1-C57 1 Blot
EUR 1086

Panel 2: Rat Minor Tissue Premade Western Blot

RW-MT-2 1 Blot
EUR 843

Panel 3: Rat Neuronal Tissue Premade Western Blot

RW-MT-3 1 Blot
EUR 601

Panel 4: Rat Digestive Tissue Premade Western Blot

RW-MT-4 1 Blot
EUR 601

Panel 5: Rat Reproductive Tissue Premade Western Blot

RW-MT-5 1 Blot
EUR 601

Panel 2: Mouse CD1 Minor Tissue Premade Western Blot

MW-MT-2 1 Blot
EUR 843

Panel 2: Mouse C57 Minor Tissue Premade Western Blot

MW-MT-2-C57 1 Blot
EUR 965

Western equine encephalitis

Oneq-VH166-OneqVH166100R Oneq-VH166-100R
EUR 1028

Western equine encephalitis

Oneq-VH166-OneqVH166150R Oneq-VH166-150R
EUR 1438

Western equine encephalitis

Oneq-VH166-OneqVH16650R Oneq-VH166-50R
EUR 728

Western equine encephalitis

PCR-VH166-PCRVH16648R PCR-VH166-48R
EUR 485

Western equine encephalitis

PCR-VH166-PCRVH16696R PCR-VH166-96R
EUR 685

Panel 3: Mouse CD1 Neuronal Tissue Premade Western Blot

MW-MT-3 1 Blot
EUR 601

Panel 3: Mouse C57 Neuronal Tissue Premade Western Blot

MW-MT-3-C57 1 Blot
EUR 722

OPRB00269-10UG - Tissue Factor (Western Blot Control) Protein

OPRB00269-10UG 10ug
EUR 256

Panel 4: Mouse CD1 Digestive Tissue Premade Western Blot

MW-MT-4 1 Blot
EUR 601

Panel 4: Mouse C57 Digestive Tissue Premade Western Blot

MW-MT-4-C57 1 Blot
EUR 722

Panel 5: Mouse CD1 Reproductive Tissue Premade Western Blot

MW-MT-5 1 Blot
EUR 601

Panel 5: Mouse C57 Reproductive Tissue Premade Western Blot

MW-MT-5-C57 1 Blot
EUR 722

Panel 6: Rat Cir, Res, Uri, Imm Tissue Premade Western Blot

RW-MT-6 1 Blot
EUR 601

Panel 6: Mouse CD1 Cir, Res, Uri, Imm Tissue Premade Western Blot

MW-MT-6 1 Blot
EUR 601

Panel 6: Mouse C57 Cir, Res, Uri, Imm Tissue Premade Western Blot

MW-MT-6-C57 1 Blot
EUR 722

Total Protein Western Blots - Human Adult Normal Tissue, Blot IV, 16 lanes

W1234404 1
EUR 1075

QPCR Kit RNA Western equine - EACH

MOL6362 EACH
EUR 1151.54

Western equine encephalitis PCR kit

PCR-VH166-48R 50T
EUR 987.6

Western equine encephalitis PCR kit

PCR-VH166-96R 100T
EUR 1335.6

OPRB00085-10UG - Tissue Inhibitor of Metalloproteinase 1 (Western Blot Cotrol) Protein

OPRB00085-10UG 10ug
EUR 256

Western equine encephalitis RT PCR kit

RTq-VH166-100R 100T
EUR 1573.2

Western equine encephalitis RT PCR kit

RTq-VH166-150R 150T
EUR 2144.4

Western equine encephalitis RT PCR kit

RTq-VH166-50R 50T
EUR 1155.6

Western equine encephalitis One-Step PCR kit

Oneq-VH166-100R 100T
EUR 1932

Western equine encephalitis One-Step PCR kit

Oneq-VH166-150R 150T
EUR 2646

Western equine encephalitis One-Step PCR kit

Oneq-VH166-50R 50T
EUR 1410

Equine Tissue cDNA Panel, any 10 Tissues

ED-010 10x10 reactions
EUR 1529

Equine Tissue Total Protein, Set of any 10 Tissues

ET-010 10x0.1mg
EUR 1188

Custome made Exosomes

Custome-made-Exosomes 5x100ug vial Ask for price

TF ELISA Kit| Equine Tissue Factor ELISA Kit

EF012674 96 Tests
EUR 826.8

Equine Tissue Genomic DNA Panel, set of any 5 Tissues

GE-005 5X0.025mg
EUR 975

Equine Tissue Genomic DNA Panel, set of any 10 Tissues

GE-010 10X0.025mg
EUR 1411

Equine Tissue Genomic DNA Panel, set of any 15 Tissues

GE-015 15X0.025mg
EUR 1702

Equine Tissue Frozen Sections Panel, Set of any 10 tissues

EF-010 10x4 slides
EUR 1088

Equine Tissue Paraffin Sections Panel, Set of any 10 tissues

EP-010 10x4 slides
EUR 943

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

DLR-TIMP1-Eq 96T
EUR 508
Description: serum, plasma, tissue homogenates or other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

DLR-TIMP1-Eq-48T 48T
EUR 667.2
Description: A sandwich quantitative ELISA assay kit for detection of Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) in samples from serum, plasma, tissue homogenates or other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

DLR-TIMP1-Eq-96T 96T
EUR 873.6
Description: A sandwich quantitative ELISA assay kit for detection of Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) in samples from serum, plasma, tissue homogenates or other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

DL-TIMP1-Eq 96T
EUR 483
Description: serum, plasma, tissue homogenates or other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

EK16929 96Т
EUR 768

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

RD-TIMP1-Eq-48T 48T
EUR 495.8
Description: serum, plasma, tissue homogenates and other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

RD-TIMP1-Eq-48Tests 48 Tests
EUR 679.2

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

RD-TIMP1-Eq-96T 96T
EUR 708.3
Description: serum, plasma, tissue homogenates and other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

RD-TIMP1-Eq-96Tests 96 Tests
EUR 944.4

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

RDR-TIMP1-Eq-48T 48T
EUR 520.59
Description: serum, plasma, tissue homogenates and other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

RDR-TIMP1-Eq-48Tests 48 Tests
EUR 709.2

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

RDR-TIMP1-Eq-96T 96T
EUR 743.72
Description: serum, plasma, tissue homogenates and other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

RDR-TIMP1-Eq-96Tests 96 Tests
EUR 987.6

Western Blot Kit

20-abx098123
  • Ask for price
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  • 100 ml
  • 200 ml

Western Blot Kit

abx098123-100l 100 µl
EUR 325

Western Blot Kit

abx098123-1ml 1 ml Ask for price

Western Blot Kit

abx098123-200l 200 µl
EUR 400

Western Blot Blocker

20960007-1 250 g
EUR 32.46

Western Blot Blocker

20960007-2 500 g
EUR 60.54

Western Blot Marker

TTOPP-2301 20Lanes
EUR 310.44

QPCR Kit RNA Western equineMmix - EACH

MOL6364 EACH
EUR 1474.68

Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

DLR-TIMP2-Eq 96T
EUR 508
Description: serum, plasma, tissue homogenates or other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

DLR-TIMP2-Eq-48T 48T
EUR 667.2
Description: A sandwich quantitative ELISA assay kit for detection of Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) in samples from serum, plasma, tissue homogenates or other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

DLR-TIMP2-Eq-96T 96T
EUR 873.6
Description: A sandwich quantitative ELISA assay kit for detection of Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) in samples from serum, plasma, tissue homogenates or other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

DLR-TIMP3-Eq 96T
EUR 508
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

DLR-TIMP3-Eq-48T 48T
EUR 667.2
Description: A sandwich quantitative ELISA assay kit for detection of Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

DLR-TIMP3-Eq-96T 96T
EUR 873.6
Description: A sandwich quantitative ELISA assay kit for detection of Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

DL-TIMP2-Eq 96T
EUR 483
Description: serum, plasma, tissue homogenates or other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

DL-TIMP3-Eq 96T
EUR 483
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

EK16934 96Т
EUR 768

Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

EK16938 96Т
EUR 768

Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

RD-TIMP2-Eq-48T 48T
EUR 495.8
Description: serum, plasma, tissue homogenates and other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

RD-TIMP2-Eq-48Tests 48 Tests
EUR 679.2

Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

RD-TIMP2-Eq-96T 96T
EUR 708.3
Description: serum, plasma, tissue homogenates and other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

RD-TIMP2-Eq-96Tests 96 Tests
EUR 944.4

Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

RD-TIMP3-Eq-48T 48T
EUR 495.8
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

RD-TIMP3-Eq-48Tests 48 Tests
EUR 679.2

Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

RD-TIMP3-Eq-96T 96T
EUR 708.3
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

RD-TIMP3-Eq-96Tests 96 Tests
EUR 944.4

Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

RDR-TIMP2-Eq-48T 48T
EUR 520.59
Description: serum, plasma, tissue homogenates and other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

RDR-TIMP2-Eq-48Tests 48 Tests
EUR 709.2

Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

RDR-TIMP2-Eq-96T 96T
EUR 743.72
Description: serum, plasma, tissue homogenates and other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

RDR-TIMP2-Eq-96Tests 96 Tests
EUR 987.6

Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

RDR-TIMP3-Eq-48T 48T
EUR 520.59
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

RDR-TIMP3-Eq-48Tests 48 Tests
EUR 709.2

Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

RDR-TIMP3-Eq-96T 96T
EUR 743.72
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

RDR-TIMP3-Eq-96Tests 96 Tests
EUR 987.6

TFPI ELISA Kit| Equine Tissue factor pathway inhibitor ELISA Kit

EF012665 96 Tests
EUR 826.8

ID4 Western Blot Kit

GWB-232633 Kit Ask for price

ERG Western Blot Kit

GWB-589385 Kit Ask for price

SSB Western Blot Kit

GWB-4759BD Kit Ask for price

FST Western Blot Kit

GWB-31B3A5 Kit Ask for price

EED Western Blot Kit

GWB-31EF96 Kit Ask for price

AHR Western Blot Kit

GWB-EFD465 Kit Ask for price

OTC Western Blot Kit

GWB-CED6C6 Kit Ask for price

ENG Western Blot Kit

GWB-C2F17D Kit Ask for price

MSI2 Western Blot Kit

GWB-24AE67 Kit Ask for price

GLI2 Western Blot Kit

GWB-2A323E Kit Ask for price

Simple Western: Bringing the Western Blot into the Twenty-First Century

The Western blot is widely used in the study of protein biochemistry, but it is notoriously labor-intensive, and it is limited in its reproducibility and quantification, among many other challenges. By contrast, capillary-based protein separation and immunodetection, known as Simple Western™, overcomes many of the challenges associated with the traditional Western blot, and it is quickly gaining traction as a replacement for traditional Western blot analysis.

The advantages that capillary-based immunoassay offers include ease of use, automation, reproducibility, quantification, and even built-in total protein normalization. In this chapter, we describe protocols for the two basic types of capillary-based immunodetection assays, one by molecular weight separation and the other by charge separation. In both methods, protein samples are separated in the capillary followed seamlessly by immunodetection with chemiluminescent or fluorescent antibodies for highly sensitive and specific detection of target proteins.

Biological Validation of a Novel Process and Product for Quantitating Western Blots

Protein normalization of western blots has relied upon housekeeping proteins which exhibit signal saturation and varied cellular expression level variations. These issues can produce spurious results leading to erroneous conclusions. A superior method to protein normalization using housekeeping proteins is Total Protein Normalization, a method now recognized as the gold standard for quantitative westerns.

Total Protein Normalization requires that all proteins on a membrane be stained or labeled uniformly, imaged, and then analyzed for total protein. It is important that such a normalization process not interfere with typical immunodetection methods, fits within existing western workflows, and exhibits a linear relationship of signal intensity to protein load under all experimental conditions. Here we report that we developed a new reagent enabling Total Protein Normalization, and we demonstrate its superior protein normalization capabilities through analysis of target proteins in different cell backgrounds.

These data illustrate how housekeeping proteins exhibit signal saturation, yield erroneous normalization data, and display sample-to-sample variations averaging 48.2% overall. Signal intensities obtained using our new method show a linear relationship to protein sample load, thus providing accurate protein normalization with an overall average variation of 7.7%.

 

Identification of Protein Carbonyls (PCOs) in Canine Serum by Western Blot Technique and Preliminary Evaluation of PCO Concentration in Dogs With Systemic Inflammation

In people, serum Protein Carbonyls (PCOs) increase during oxidative stress (OS) due to oxidative damage to proteins. OS is often associated with inflammation and especially with sepsis, a condition hard to diagnose in veterinary medicine because reliable markers are lacking. The aim of this study was to assess whether PCOs in canine serum may be detected by antibody-based methods such as Western Blotting (WB), and to preliminarily investigate the possible utility of this marker in dogs with inflammation.

A serum sample oxidized in vitro was used to set up the method; the coefficient of variation obtained by repeated analysis varied from 24 to 36%. In order to assess whether the technique may cover the range of PCOs concentration detectable in routine practice, PCOs were measured in 4 healthy dogs and in 15 with inflammatory diseases, in some cases potentially associated with sepsis, as suggested by the results of other inflammatory markers such as C-Reactive Protein (CRP) and the anti-oxidant enzyme Paraoxonase 1 (PON-1): the concentration of PCOs was low in dogs with normal PON-1 activity,

moderately increased in the majority of dogs with low-normal PON-1 activity, and severely increased in dogs with very low PON-1 activity. In conclusion this study demonstrates that PCOs, may be detected in canine serum, using antibody-based techniques such as WB. The preliminary results in dogs with and without systemic inflammation encourage further studies on the possible role of PCOs as inflammatory markers.

 

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