Identification of early diagnostic antigens in soluble proteins of Trichinella spiralis

Identification of early diagnostic antigens in soluble proteins of Trichinella spiralis

Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Mitochondrial Respiratory Chain Complexes

Cardiac, neuronal and renal tubular epithelial cells are the most metabolically active cells in the body. Their fate depends largely on their mitochondria as the primary energy generating system which participates in the control of apoptosis, cell cycle and metabolism. Thus, mitochondrial dysfunction is a hallmark of many chronic diseases including diabetic nephropathy. A drop in mitochondrial bioenergetics efficiency is often associated with altered expression of respiratory chain complexes. Moreover, recent studies demonstrate that cellular proteins can shuttle to mitochondria and modify their function directly.

Here we illustrate two mitochondria isolation protocols; one is recommended if the purity of the mitochondrial fraction is a priority such as if the mitochondrial localization of a protein has to be validated, the other if a high yield of intact functional mitochondria is required for functional studies and quantitative Western blotting. Next, we provide a detailed protocol for Western blotting of isolated mitochondria and renal cortex either to prove the purity of isolated fractions or to quantify complexes of the mitochondrial respiratory chain. We used this approach to identify classically cell membrane bound angiotensin II receptors in mitochondria and to study the effect of these receptors on mitochondrial function in early stages of diabetic nephropathy.

Identification of early diagnostic antigens in soluble proteins of Trichinella spiralis adult worms by Western blot

Previous studies showed that crude antigens from Trichinella spiralis adult worms (AW) can be recognized by mouse infection sera at 8 days post infection. The aim of this study was to identify the early diagnostic antigenic bands in soluble proteins from T. spiralis AW by Western blot using early infection sera. The affecting factors of adult recovery were firstly observed in this study, and the results showed that the maximum number of adults was collected from small intestine when the female BALB/c mice were orally infected with 4000 ML and sacrificed at 3 days post infection.

The results of Western blot analysis showed that seven protein bands (31, 35.1, 39, 40.6, 41.9, 47 and 50.6 kDa) could be recognized by early infection sera as early as at 8-10 days post infection, and were strongly reacted with mouse infection sera at 11-12 days post infection. Our results suggested that the seven protein bands of T. spiralis AW soluble proteins might be the early expressed antigens during the intestinal stage of Trichinella infection and therefore have potential value for the early diagnosis of trichinellosis.

nwreprosci
nwreprosci

TMB Substrate, for Western Blot

F100029 1000 ml
EUR 992.4
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

TMB Substrate, for Western Blot

F129-100 100 ml
EUR 298.8
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

Interferon alpha Western Blot Antibody

20-abx137014
  • EUR 427.20
  • EUR 627.60
  • 0.5 mg
  • 1 mg

PDCD4 Western Blot kit (AWBK09045)

AWBK09045 10 reactions
EUR 776.4
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ETS1 Western Blot kit (AWBK100604)

AWBK100604 10 reactions
EUR 776.4
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ERG Western Blot kit (AWBK100625)

AWBK100625 10 reactions
EUR 776.4
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GABRP Western Blot kit (AWBK13034)

AWBK13034 10 reactions
EUR 776.4
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HTR1A Western Blot kit (AWBK13041)

AWBK13041 10 reactions
EUR 776.4
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RAB14 Western Blot kit (AWBK13107)

AWBK13107 10 reactions
EUR 776.4
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TNRC4 Western Blot kit (AWBK31525)

AWBK31525 10 reactions
EUR 776.4
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ESRRG Western Blot kit (AWBK31655)

AWBK31655 10 reactions
EUR 776.4
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GLI2 Western Blot kit (AWBK31885)

AWBK31885 10 reactions
EUR 776.4
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PAX4 Western Blot kit (AWBK32064)

AWBK32064 10 reactions
EUR 776.4
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AHR Western Blot kit (AWBK32243)

AWBK32243 10 reactions
EUR 776.4
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GLI1 Western Blot kit (AWBK32368)

AWBK32368 10 reactions
EUR 776.4
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PAX7 Western Blot kit (AWBK32393)

AWBK32393 10 reactions
EUR 776.4
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LHX6 Western Blot kit (AWBK32553)

AWBK32553 10 reactions
EUR 776.4
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HIPK2 Western Blot kit (AWBK32586)

AWBK32586 10 reactions
EUR 776.4
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PAX7 Western Blot kit (AWBK32742)

AWBK32742 10 reactions
EUR 776.4
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ZFP67 Western Blot kit (AWBK32749)

AWBK32749 10 reactions
EUR 776.4
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OLIG2 Western Blot kit (AWBK32753)

AWBK32753 10 reactions
EUR 776.4
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ENG Western Blot kit (AWBK33068)

AWBK33068 10 reactions
EUR 776.4
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SOX5 Western Blot kit (AWBK33323)

AWBK33323 10 reactions
EUR 776.4
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SOX10 Western Blot kit (AWBK33326)

AWBK33326 10 reactions
EUR 776.4
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RPS16 Western Blot kit (AWBK33535)

AWBK33535 10 reactions
EUR 776.4
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ATP7A Western Blot kit (AWBK33797)

AWBK33797 10 reactions
EUR 776.4
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A1BG Western Blot kit (AWBK33810)

AWBK33810 10 reactions
EUR 776.4
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NPM1 Western Blot kit (AWBK34094)

AWBK34094 10 reactions
EUR 776.4
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LIG4 Western Blot kit (AWBK34122)

AWBK34122 10 reactions
EUR 776.4
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RNF2 Western Blot kit (AWBK34290)

AWBK34290 10 reactions
EUR 776.4
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ZFP36 Western Blot kit (AWBK34385)

AWBK34385 10 reactions
EUR 776.4
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ARIH2 Western Blot kit (AWBK34418)

AWBK34418 10 reactions
EUR 776.4
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KEAP1 Western Blot kit (AWBK34728)

AWBK34728 10 reactions
EUR 776.4
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CLIC1 Western Blot kit (AWBK35045)

AWBK35045 10 reactions
EUR 776.4
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KCNN2 Western Blot kit (AWBK35094)

AWBK35094 10 reactions
EUR 776.4
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CLCN3 Western Blot kit (AWBK35504)

AWBK35504 10 reactions
EUR 776.4
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P2RX7 Western Blot kit (AWBK35518)

AWBK35518 10 reactions
EUR 776.4
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TRPM3 Western Blot kit (AWBK35609)

AWBK35609 10 reactions
EUR 776.4
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PITX2 Western Blot kit (AWBK35634)

AWBK35634 10 reactions
EUR 776.4
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ILF2 Western Blot kit (AWBK35731)

AWBK35731 10 reactions
EUR 776.4
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ONECUT2 Western Blot kit (AWBK35750)

AWBK35750 10 reactions
EUR 776.4
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ZFP42 Western Blot kit (AWBK36244)

AWBK36244 10 reactions
EUR 776.4
Description:
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ANXA1 Western Blot kit (AWBK36568)

AWBK36568 10 reactions
EUR 776.4
Description:
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SPP1 Western Blot kit (AWBK36677)

AWBK36677 10 reactions
EUR 776.4
Description:
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RUNX2 Western Blot kit (AWBK36678)

AWBK36678 10 reactions
EUR 776.4
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ZBP1 Western Blot kit (AWBK36711)

AWBK36711 10 reactions
EUR 776.4
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Bapx1 Western Blot kit (AWBK36826)

AWBK36826 10 reactions
EUR 776.4
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G3BP Western Blot kit (AWBK37713)

AWBK37713 10 reactions
EUR 776.4
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TWIST1 Western Blot kit (AWBK37997)

AWBK37997 10 reactions
EUR 776.4
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ID4 Western Blot kit (AWBK38058)

AWBK38058 10 reactions
EUR 776.4
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SATB1 Western Blot kit (AWBK38208)

AWBK38208 10 reactions
EUR 776.4
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EED Western Blot kit (AWBK38384)

AWBK38384 10 reactions
EUR 776.4
Description:
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TARDBP Western Blot kit (AWBK38941)

AWBK38941 10 reactions
EUR 776.4
Description:
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TARDBP Western Blot kit (AWBK38942)

AWBK38942 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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BRD7 Western Blot kit (AWBK39018)

AWBK39018 10 reactions
EUR 776.4
Description:
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JMJD3 Western Blot kit (AWBK40102)

AWBK40102 10 reactions
EUR 776.4
Description:
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SNRP70 Western Blot kit (AWBK40439)

AWBK40439 10 reactions
EUR 776.4
Description:
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SSB Western Blot kit (AWBK40462)

AWBK40462 10 reactions
EUR 776.4
Description:
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STAU1 Western Blot kit (AWBK40537)

AWBK40537 10 reactions
EUR 776.4
Description:
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PCBP1 Western Blot kit (AWBK40630)

AWBK40630 10 reactions
EUR 776.4
Description:
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HNRPLL Western Blot kit (AWBK41102)

AWBK41102 10 reactions
EUR 776.4
Description:
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MSI2 Western Blot kit (AWBK41112)

AWBK41112 10 reactions
EUR 776.4
Description:
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HNRPA3 Western Blot kit (AWBK41195)

AWBK41195 10 reactions
EUR 776.4
Description:
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WNT2B Western Blot kit (AWBK41254)

AWBK41254 10 reactions
EUR 776.4
Description:
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GNB1L Western Blot kit (AWBK41328)

AWBK41328 10 reactions
EUR 776.4
Description:
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SLC12A1 Western Blot kit (AWBK41388)

AWBK41388 10 reactions
EUR 776.4
Description:
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TPM1 Western Blot kit (AWBK41392)

AWBK41392 10 reactions
EUR 776.4
Description:
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CEACAM6 Western Blot kit (AWBK41504)

AWBK41504 10 reactions
EUR 776.4
Description:
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SLC22A1 Western Blot kit (AWBK41516)

AWBK41516 10 reactions
EUR 776.4
Description:
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STK11 Western Blot kit (AWBK41743)

AWBK41743 10 reactions
EUR 776.4
Description:
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OTC Western Blot kit (AWBK41766)

AWBK41766 10 reactions
EUR 776.4
Description:
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TSHR Western Blot kit (AWBK41870)

AWBK41870 10 reactions
EUR 776.4
Description:
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RHOBTB1 Western Blot kit (AWBK41883)

AWBK41883 10 reactions
EUR 776.4
Description:
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KRT13 Western Blot kit (AWBK42031)

AWBK42031 10 reactions
EUR 776.4
Description:
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IDH3A Western Blot kit (AWBK42237)

AWBK42237 10 reactions
EUR 776.4
Description:
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SLC1A5 Western Blot kit (AWBK42247)

AWBK42247 10 reactions
EUR 776.4
Description:
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PBEF1 Western Blot kit (AWBK42255)

AWBK42255 10 reactions
EUR 776.4
Description:
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FST Western Blot kit (AWBK42287)

AWBK42287 10 reactions
EUR 776.4
Description:
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SLC22A7 Western Blot kit (AWBK42306)

AWBK42306 10 reactions
EUR 776.4
Description:
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ASPN Western Blot kit (AWBK42487)

AWBK42487 10 reactions
EUR 776.4
Description:
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RSAD2 Western Blot kit (AWBK42696)

AWBK42696 10 reactions
EUR 776.4
Description:
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PANX2 Western Blot kit (AWBK42778)

AWBK42778 10 reactions
EUR 776.4
Description:
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PANX1 Western Blot kit (AWBK42783)

AWBK42783 10 reactions
EUR 776.4
Description:
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TGFBI Western Blot kit (AWBK44268)

AWBK44268 10 reactions
EUR 776.4
Description:
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PTPN2 Western Blot kit (AWBK45362)

AWBK45362 10 reactions
EUR 776.4
Description:
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RORA Western Blot kit (AWBK45608)

AWBK45608 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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Chemiluminescent Western Blot Kit (OKRA00001)

OKRA00001 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00002)

OKRA00002 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00003)

OKRA00003 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00004)

OKRA00004 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00014)

OKRA00014 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00015)

OKRA00015 1 each
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00016)

OKRA00016 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00017)

OKRA00017 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Western equine encephalitis PCR kit

PCR-VH166-48R 50T
EUR 987.6

Western equine encephalitis PCR kit

PCR-VH166-96R 100T
EUR 1335.6

QPCR Kit RNA Western equine

MOL6362 EACH
EUR 820.8

Western Blot Primary Antibody Diluent Buffer

abx090689-100ml 100 ml
EUR 218.4

cyclin G2 Western Blot kit (AWBK03032)

AWBK03032 10 reactions
EUR 776.4
Description:
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  • Species reactivity:
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Attoglow Western Blot Analysis Kit:Blocking Agent

K3171250-3 40 g
EUR 130.8
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

T-Pro Western Blot Stripping Reagent

JB11-K002 500ml/BT
EUR 213.6

Pure-IP Western Blot Detection Kit

PRB-5002 20 blots
EUR 470.4
Description: Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection Kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody.

Bovine DNAse 1 Protein Western blot +ve control

DNASE11-C 100 ul
EUR 343.2

Mouse Leptin protein Western blot +ve control # 1

LEP11-C 100 ul
EUR 343.2

Total Protein Western Blots - Human Adult Normal Tissue, Blot IV, 16 lanes

W1234404 1
EUR 1120.8
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Pre-made Minicircle CMV-GFP-SV40PolyA (positive control)

MN601MC-1 40 ug
EUR 882

Pre-made Minicircle CMV-rRFP-SV40PolyA (positive control)

MN602MC-1 40 ug
EUR 882

Western equine encephalitis RT PCR kit

RTq-VH166-100R 100T
EUR 1573.2

Western equine encephalitis RT PCR kit

RTq-VH166-150R 150T
EUR 2144.4

Western equine encephalitis RT PCR kit

RTq-VH166-50R 50T
EUR 1155.6

Mouse Ceruloplasmin protein control for western blot

CP16-C 100 ul
EUR 343.2

Rat Ceruloplasmin protein control for western blot

CP17-C 100 ul
EUR 343.2

Poly-ADP-ribosylated proteins for Western blot

ADPR11-C 100 ul
EUR 343.2

SuperBrite? ECL Western Blot Substrate/Detection Kit

K824-50
EUR 229.2

Recombinant Human MutY Protein for Western blot

MUTY11-C 100 ul
EUR 343.2

Nitrated Mol Wt Std for Western blot

NITT12-C 100 ul
EUR 343.2

Attoglow Western Blot Analysis Kit:Binding Buffer 20x

K3171250-2 120 ml
EUR 171.6
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Box Western Blot Polystyrene Transparent 95x30x16mm X5

DD62676 PK5
EUR 94.62

Purified Ram Cyclooxygenase (Cox-1) Western blot +ve control

COX11-C 100 ul
EUR 343.2

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-050 500ml
EUR 144

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-100 2x500ml
EUR 176.4

Custom Neg Control siRNA

M1256-1
EUR 333.6

Custom Pos Control siRNA

M1258-1
EUR 333.6

Human Normal Tissue Blot II

1522 One blot
EUR 544.2
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, heart, kidney, liver, lung, pancreas, skeletal muscle, skin, and spleen.

Human Normal Tissue Blot VI

1526 One blot
EUR 544.2
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The human normal tissues included in the blot are left cerebellum, right cerebellum, frontal lobe, occipital lobe, parietal lobe, whole eye, spinal cord, temporal lobe, and thalamus.

Human Tumor Tissue Blot I

1542 One blot
EUR 638.7
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human tumor tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human tumor tissues included in the blot are brain, colon, kidney, liver, lung, pancreas, skin, spleen, and stomach.

Human Tumor Tissue Blot II

1543 One blot
EUR 638.7
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human tumor tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The human tumor tissues included in the blot are colon, duodenum, esophagus, small intestine, liver, pancreas, rectum, and stomach.

Human Tumor Tissue Blot III

1544 One blot
EUR 638.7
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human tumor tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The human tumor tissues included in the blot are bladder, breast, kidney, ovary, prostate, testis, cervix, and uterus.

Human Tumor Tissue Blot IV

1545 One blot
EUR 638.7
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human tumor tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human tumor tissues included in the blot are kidney, liver, lymphoma, Non-Hodgkin's lymphoma, spleen, thymoma, thyroid, and tonsil.

Mouse Normal Tissue Blot I

1561 One blot
EUR 493.8
Description: The mouse tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different mouse tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The mouse tissues included in the blot are brain, colon, heart, kidney, liver, lung, pancreas, and spleen.

Mouse Normal Tissue Blot II

1562 One blot
EUR 493.8
Description: The mouse tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different mouse tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The mouse tissues included in the blot are adrenal, cerebellum, cerebrum, small intestine, skeletal muscle, stomach, testis, and thymus.

Rat Normal Tissue Blot I

1581 One blot
EUR 493.8
Description: The rat tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The rat tissues included in the blot are brain, colon, heart, kidney, liver, lung, pancreas, and spleen.

Rat Normal Tissue Blot II

1582 One blot
EUR 493.8
Description: The rat tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The rat tissues included in the blot are adrenal, cerebellum, cerebrum, small intestine, skeletal muscle, stomach, testis, and thymus.

Custom Assortment of Mounts and Loops *please specify sizes* qty 20 total pins

M-L18SP-Custom 20 MOUNTS
EUR 123
Description: Custom Assortment of Mounts and Loops *please specify sizes* qty 20 total pins

Western equine encephalitis One-Step PCR kit

Oneq-VH166-100R 100T
EUR 1932

Western equine encephalitis One-Step PCR kit

Oneq-VH166-150R 150T
EUR 2646

Western equine encephalitis One-Step PCR kit

Oneq-VH166-50R 50T
EUR 1410

Human C1 Esterase Inhibitor western blot +ve control

C1E11-C 100 ul
EUR 343.2

Purified Human C1q protein Western blot +ve control

C1Q11-C 100 ul
EUR 343.2

Purified Cholera Toxin protein control for Western Blot

CTOX11-C 100 ul
EUR 343.2

Chicken Serum Albumin protein control for Western blot

ALBC15-C 100 ul
EUR 343.2

Human Spleen Ferritin protein Western blot +ve control

FERT11-C 100 ul
EUR 343.2

Human Liver Ferritin protein Western blot +ve control

FERT12-C 100 ul
EUR 343.2

Horse Spleen Ferritin protein Western blot +ve control

FERT13-C 100 ul
EUR 343.2

Western blot recycling stripping buffer, 50 ml (10X)

90101 50 ml
EUR 285.6

ChonBlock Western Blot Buffer 2 for Goat (PBS)

90691P 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Goat (PBS)

ChonBlock Western Blot Buffer 2 for Goat (TBS)

90691T 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Goat (TBS)

ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

90692P 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

90692T 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

Beta-Galactosidase (E. Coli) Protein for Western Blot

BGAL11-C 100 ul
EUR 343.2

Recombinant Beta Lactamase protein control for Western blot

BLAC11-C 100 ul
EUR 343.2

Human Leptin protein Western blot +ve control # 2

LEP12-C 100 ul
EUR 343.2

HRP Western Blot Anti-IgG Antibody Kit (OKRA00006)

OKRA00006 100 ug
EUR 246
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Human Pancreatic Amylase protein control for Western blot

PAMY13-C 100 ul
EUR 343.2

Custom Peptide Synthesis order form

Pepord-1 1 Ask for price

Equine IFN beta 1 Recombinant Protein

R02041-1 5ug/vial
EUR 310.8
Description: IFN beta is a mammalian Type I inferferon, functionig as a regulator of cellular activity by interacting with cell-surface receptors and activating various signaling pathways. IFN beta produces antiviral, antibacterial, and anticancer properties. Equine IFN beta Recombinant Protein is purified IFN beta produced in yeast.

Recombinant 1 Envelope glycoprotein 2 (1 E2) Protein control for western blot

BVDE11-C 100 ul
EUR 343.2

Mouse NOS-II/i-NOS Protein Western Blot +ve Control # 1

iNOS-C 100 ul
EUR 343.2

Published references using ADI custom services

CPUB-1 1 Ask for price

Recombinant Norovirus 1 (MNV-1) Capsid Protein 1 (VP1) control for Western blot

MNV14-C 100 ul
EUR 343.2

Camelpox virus H3L/p35 protein control for western blot

CPOX11-C 100 ul
EUR 343.2

Human Liver Cathepsin B protein control for Western blot

CTHB11-C 100 ul
EUR 343.2

Human Liver Cathepsin D protein control for Western blot

CTHD11-C 100 ul
EUR 343.2

Human Liver Cathepsin G protein control for Western blot

CTHG11-C 100 ul
EUR 343.2

Human Liver Cathepsin H protein control for Western blot

CTHH11-C 100 ul
EUR 343.2

Human Liver Cathepsin L protein control for Western blot

CTHL11-C 100 ul
EUR 343.2

Purified Ram Cyclooxygenase (Cox-2) Western blot +ve control

COX21-C 100 ul
EUR 343.2

WesternOne? Marker Pen, HRP for Western blot chemiluminescent detection

ACT-WP
EUR 172.8

Human Adipsin/Factor D protein Western blot +ve control

ADN12-C 100 ul
EUR 343.2

Purified Mouse serum albumin protein control for Western blot

ALB11-C 100 ul
EUR 343.2

Purified Human serum albumin protein control for Western blot

ALBH13-C 100 ul
EUR 343.2

Purified rat serum albumin protein control for Western blot

ALBR12-C 100 ul
EUR 343.2

Purified Sheep serum albumin protein control for Western blot

ALBSH20-C 100 ul
EUR 343.2

Western blot Kit for Rabbit Primary Antibodies, Chemilum. Substrate

80200-Rb 1 kit
EUR 781.2

Western blot Kit for Goat Primary Antibodies, Chemilum. Substrate

80201-Gt 1 kit
EUR 781.2

Western blot Kit for Mouse Primary Antibodies, Chemilum. Substrate

80202-Mo 1 kit
EUR 781.2

Western blot Kit for Rat Primary Antibodies, Chemilum. Substrate

80203-Rt 1 kit
EUR 781.2

Western blot Kit for Chicken Primary Antibodies, Chemilum. Substrate

80204-Ch 1 kit
EUR 781.2

Western blot Kit for Monkey Primary Antibodies, Chemilum. Substrate

80205-Mk 1 kit
EUR 781.2

Western blot Kit for Human Primary Antibodies, Chemilum. Substrate

80207-Hu 1 kit
EUR 781.2

Western blot Kit for Sheep Primary Antibodies; Chemilum. Substrate

80208-Sh 1 kit
EUR 781.2

Western blot Kit for Pig Primary Antibodies; Chemilum. Substrate

80209-Pg 1 kit
EUR 781.2

NuGlo ECL Substrate Kit for Western blot, 400 mls

80215 1 kit
EUR 489.6

Simple Western: Bringing the Western Blot into the Twenty-First Century

The Western blot is widely used in the study of protein biochemistry, but it is notoriously labor-intensive, and it is limited in its reproducibility and quantification, among many other challenges. By contrast, capillary-based protein separation and immunodetection, known as Simple Western™, overcomes many of the challenges associated with the traditional Western blot, and it is quickly gaining traction as a replacement for traditional Western blot analysis.

The advantages that capillary-based immunoassay offers include ease of use, automation, reproducibility, quantification, and even built-in total protein normalization. In this chapter, we describe protocols for the two basic types of capillary-based immunodetection assays, one by molecular weight separation and the other by charge separation. In both methods, protein samples are separated in the capillary followed seamlessly by immunodetection with chemiluminescent or fluorescent antibodies for highly sensitive and specific detection of target proteins.

Biological Validation of a Novel Process and Product for Quantitating Western Blots

Protein normalization of western blots has relied upon housekeeping proteins which exhibit signal saturation and varied cellular expression level variations. These issues can produce spurious results leading to erroneous conclusions. A superior method to protein normalization using housekeeping proteins is Total Protein Normalization, a method now recognized as the gold standard for quantitative westerns.

Total Protein Normalization requires that all proteins on a membrane be stained or labeled uniformly, imaged, and then analyzed for total protein. It is important that such a normalization process not interfere with typical immunodetection methods, fits within existing western workflows, and exhibits a linear relationship of signal intensity to protein load under all experimental conditions. Here we report that we developed a new reagent enabling Total Protein Normalization, and we demonstrate its superior protein normalization capabilities through analysis of target proteins in different cell backgrounds.

These data illustrate how housekeeping proteins exhibit signal saturation, yield erroneous normalization data, and display sample-to-sample variations averaging 48.2% overall. Signal intensities obtained using our new method show a linear relationship to protein sample load, thus providing accurate protein normalization with an overall average variation of 7.7%.

 

Identification of Protein Carbonyls (PCOs) in Canine Serum by Western Blot Technique and Preliminary Evaluation of PCO Concentration in Dogs With Systemic Inflammation

In people, serum Protein Carbonyls (PCOs) increase during oxidative stress (OS) due to oxidative damage to proteins. OS is often associated with inflammation and especially with sepsis, a condition hard to diagnose in veterinary medicine because reliable markers are lacking. The aim of this study was to assess whether PCOs in canine serum may be detected by antibody-based methods such as Western Blotting (WB), and to preliminarily investigate the possible utility of this marker in dogs with inflammation.

A serum sample oxidized in vitro was used to set up the method; the coefficient of variation obtained by repeated analysis varied from 24 to 36%. In order to assess whether the technique may cover the range of PCOs concentration detectable in routine practice, PCOs were measured in 4 healthy dogs and in 15 with inflammatory diseases, in some cases potentially associated with sepsis, as suggested by the results of other inflammatory markers such as C-Reactive Protein (CRP) and the anti-oxidant enzyme Paraoxonase 1 (PON-1): the concentration of PCOs was low in dogs with normal PON-1 activity,

moderately increased in the majority of dogs with low-normal PON-1 activity, and severely increased in dogs with very low PON-1 activity. In conclusion this study demonstrates that PCOs, may be detected in canine serum, using antibody-based techniques such as WB. The preliminary results in dogs with and without systemic inflammation encourage further studies on the possible role of PCOs as inflammatory markers.

 

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