Identification of early diagnostic antigens in soluble proteins of Trichinella spiralis

Identification of early diagnostic antigens in soluble proteins of Trichinella spiralis

Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Mitochondrial Respiratory Chain Complexes

Cardiac, neuronal and renal tubular epithelial cells are the most metabolically active cells in the body. Their fate depends largely on their mitochondria as the primary energy generating system which participates in the control of apoptosis, cell cycle and metabolism. Thus, mitochondrial dysfunction is a hallmark of many chronic diseases including diabetic nephropathy. A drop in mitochondrial bioenergetics efficiency is often associated with altered expression of respiratory chain complexes. Moreover, recent studies demonstrate that cellular proteins can shuttle to mitochondria and modify their function directly.

Here we illustrate two mitochondria isolation protocols; one is recommended if the purity of the mitochondrial fraction is a priority such as if the mitochondrial localization of a protein has to be validated, the other if a high yield of intact functional mitochondria is required for functional studies and quantitative Western blotting. Next, we provide a detailed protocol for Western blotting of isolated mitochondria and renal cortex either to prove the purity of isolated fractions or to quantify complexes of the mitochondrial respiratory chain. We used this approach to identify classically cell membrane bound angiotensin II receptors in mitochondria and to study the effect of these receptors on mitochondrial function in early stages of diabetic nephropathy.

Identification of early diagnostic antigens in soluble proteins of Trichinella spiralis adult worms by Western blot

Previous studies showed that crude antigens from Trichinella spiralis adult worms (AW) can be recognized by mouse infection sera at 8 days post infection. The aim of this study was to identify the early diagnostic antigenic bands in soluble proteins from T. spiralis AW by Western blot using early infection sera. The affecting factors of adult recovery were firstly observed in this study, and the results showed that the maximum number of adults was collected from small intestine when the female BALB/c mice were orally infected with 4000 ML and sacrificed at 3 days post infection.

The results of Western blot analysis showed that seven protein bands (31, 35.1, 39, 40.6, 41.9, 47 and 50.6 kDa) could be recognized by early infection sera as early as at 8-10 days post infection, and were strongly reacted with mouse infection sera at 11-12 days post infection. Our results suggested that the seven protein bands of T. spiralis AW soluble proteins might be the early expressed antigens during the intestinal stage of Trichinella infection and therefore have potential value for the early diagnosis of trichinellosis.

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TF ELISA Kit| Equine Tissue Factor ELISA Kit

EF012674 96 Tests
EUR 826.8

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

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EUR 667.2
Description: A sandwich quantitative ELISA assay kit for detection of Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) in samples from serum, plasma, tissue homogenates or other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

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Description: A sandwich quantitative ELISA assay kit for detection of Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) in samples from serum, plasma, tissue homogenates or other biological fluids.

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

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Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

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Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

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Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

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Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

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20-abx098123
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Equine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

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Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

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Description: serum, plasma, tissue homogenates or other biological fluids.

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Western Blot Marker

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AHR Western Blot kit (AWBK32243)

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ENG Western Blot kit (AWBK33068)

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ID4 Western Blot kit (AWBK38058)

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EED Western Blot kit (AWBK38384)

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SSB Western Blot kit (AWBK40462)

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OTC Western Blot kit (AWBK41766)

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EUR 992.4
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ERG Western Blot kit (AWBK100625)

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GLI2 Western Blot kit (AWBK31885)

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PAX4 Western Blot kit (AWBK32064)

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GLI1 Western Blot kit (AWBK32368)

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PAX7 Western Blot kit (AWBK32393)

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LHX6 Western Blot kit (AWBK32553)

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EUR 776.4
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PAX7 Western Blot kit (AWBK32742)

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SOX5 Western Blot kit (AWBK33323)

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EUR 776.4
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A1BG Western Blot kit (AWBK33810)

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EUR 776.4
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NPM1 Western Blot kit (AWBK34094)

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EUR 776.4
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LIG4 Western Blot kit (AWBK34122)

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EUR 776.4
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RNF2 Western Blot kit (AWBK34290)

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EUR 776.4
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ILF2 Western Blot kit (AWBK35731)

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EUR 776.4
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SPP1 Western Blot kit (AWBK36677)

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EUR 776.4
Description:
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ZBP1 Western Blot kit (AWBK36711)

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EUR 776.4
Description:
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G3BP Western Blot kit (AWBK37713)

AWBK37713 10 reactions
EUR 776.4
Description:
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BRD7 Western Blot kit (AWBK39018)

AWBK39018 10 reactions
EUR 776.4
Description:
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MSI2 Western Blot kit (AWBK41112)

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EUR 776.4
Description:
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TPM1 Western Blot kit (AWBK41392)

AWBK41392 10 reactions
EUR 776.4
Description:
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TSHR Western Blot kit (AWBK41870)

AWBK41870 10 reactions
EUR 776.4
Description:
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ASPN Western Blot kit (AWBK42487)

AWBK42487 10 reactions
EUR 776.4
Description:
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RORA Western Blot kit (AWBK45608)

AWBK45608 10 reactions
EUR 776.4
Description:
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PDCD4 Western Blot kit (AWBK09045)

AWBK09045 10 reactions
EUR 776.4
Description:
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ETS1 Western Blot kit (AWBK100604)

AWBK100604 10 reactions
EUR 776.4
Description:
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GABRP Western Blot kit (AWBK13034)

AWBK13034 10 reactions
EUR 776.4
Description:
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HTR1A Western Blot kit (AWBK13041)

AWBK13041 10 reactions
EUR 776.4
Description:
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RAB14 Western Blot kit (AWBK13107)

AWBK13107 10 reactions
EUR 776.4
Description:
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TNRC4 Western Blot kit (AWBK31525)

AWBK31525 10 reactions
EUR 776.4
Description:
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ESRRG Western Blot kit (AWBK31655)

AWBK31655 10 reactions
EUR 776.4
Description:
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HIPK2 Western Blot kit (AWBK32586)

AWBK32586 10 reactions
EUR 776.4
Description:
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ZFP67 Western Blot kit (AWBK32749)

AWBK32749 10 reactions
EUR 776.4
Description:
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OLIG2 Western Blot kit (AWBK32753)

AWBK32753 10 reactions
EUR 776.4
Description:
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SOX10 Western Blot kit (AWBK33326)

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EUR 776.4
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RPS16 Western Blot kit (AWBK33535)

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EUR 776.4
Description:
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ATP7A Western Blot kit (AWBK33797)

AWBK33797 10 reactions
EUR 776.4
Description:
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ZFP36 Western Blot kit (AWBK34385)

AWBK34385 10 reactions
EUR 776.4
Description:
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ARIH2 Western Blot kit (AWBK34418)

AWBK34418 10 reactions
EUR 776.4
Description:
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KEAP1 Western Blot kit (AWBK34728)

AWBK34728 10 reactions
EUR 776.4
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CLIC1 Western Blot kit (AWBK35045)

AWBK35045 10 reactions
EUR 776.4
Description:
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KCNN2 Western Blot kit (AWBK35094)

AWBK35094 10 reactions
EUR 776.4
Description:
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CLCN3 Western Blot kit (AWBK35504)

AWBK35504 10 reactions
EUR 776.4
Description:
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P2RX7 Western Blot kit (AWBK35518)

AWBK35518 10 reactions
EUR 776.4
Description:
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TRPM3 Western Blot kit (AWBK35609)

AWBK35609 10 reactions
EUR 776.4
Description:
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PITX2 Western Blot kit (AWBK35634)

AWBK35634 10 reactions
EUR 776.4
Description:
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ZFP42 Western Blot kit (AWBK36244)

AWBK36244 10 reactions
EUR 776.4
Description:
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ANXA1 Western Blot kit (AWBK36568)

AWBK36568 10 reactions
EUR 776.4
Description:
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RUNX2 Western Blot kit (AWBK36678)

AWBK36678 10 reactions
EUR 776.4
Description:
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Bapx1 Western Blot kit (AWBK36826)

AWBK36826 10 reactions
EUR 776.4
Description:
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SATB1 Western Blot kit (AWBK38208)

AWBK38208 10 reactions
EUR 776.4
Description:
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JMJD3 Western Blot kit (AWBK40102)

AWBK40102 10 reactions
EUR 776.4
Description:
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STAU1 Western Blot kit (AWBK40537)

AWBK40537 10 reactions
EUR 776.4
Description:
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PCBP1 Western Blot kit (AWBK40630)

AWBK40630 10 reactions
EUR 776.4
Description:
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WNT2B Western Blot kit (AWBK41254)

AWBK41254 10 reactions
EUR 776.4
Description:
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GNB1L Western Blot kit (AWBK41328)

AWBK41328 10 reactions
EUR 776.4
Description:
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STK11 Western Blot kit (AWBK41743)

AWBK41743 10 reactions
EUR 776.4
Description:
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KRT13 Western Blot kit (AWBK42031)

AWBK42031 10 reactions
EUR 776.4
Description:
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IDH3A Western Blot kit (AWBK42237)

AWBK42237 10 reactions
EUR 776.4
Description:
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PBEF1 Western Blot kit (AWBK42255)

AWBK42255 10 reactions
EUR 776.4
Description:
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RSAD2 Western Blot kit (AWBK42696)

AWBK42696 10 reactions
EUR 776.4
Description:
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PANX2 Western Blot kit (AWBK42778)

AWBK42778 10 reactions
EUR 776.4
Description:
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PANX1 Western Blot kit (AWBK42783)

AWBK42783 10 reactions
EUR 776.4
Description:
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TGFBI Western Blot kit (AWBK44268)

AWBK44268 10 reactions
EUR 776.4
Description:
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PTPN2 Western Blot kit (AWBK45362)

AWBK45362 10 reactions
EUR 776.4
Description:
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TWIST1 Western Blot kit (AWBK37997)

AWBK37997 10 reactions
EUR 776.4
Description:
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TARDBP Western Blot kit (AWBK38941)

AWBK38941 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TARDBP Western Blot kit (AWBK38942)

AWBK38942 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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SNRP70 Western Blot kit (AWBK40439)

AWBK40439 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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HNRPLL Western Blot kit (AWBK41102)

AWBK41102 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

HNRPA3 Western Blot kit (AWBK41195)

AWBK41195 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC1A5 Western Blot kit (AWBK42247)

AWBK42247 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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ONECUT2 Western Blot kit (AWBK35750)

AWBK35750 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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SLC12A1 Western Blot kit (AWBK41388)

AWBK41388 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

CEACAM6 Western Blot kit (AWBK41504)

AWBK41504 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC22A1 Western Blot kit (AWBK41516)

AWBK41516 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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RHOBTB1 Western Blot kit (AWBK41883)

AWBK41883 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC22A7 Western Blot kit (AWBK42306)

AWBK42306 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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Interferon alpha Western Blot Antibody

20-abx137014
  • EUR 427.20
  • EUR 627.60
  • 0.5 mg
  • 1 mg

TBS for Western Blot 10X, pH 7.4

42020384-1 1 L Ask for price

TBS for Western Blot 10X, pH 7.4

42020384-2 2 L Ask for price

TBS for Western Blot 10X, pH 7.4

42020384-3 4 L Ask for price

TBS for Western Blot 10X, pH 7.4

42020384-4 500 mL Ask for price

Colorimetric Western Blot Kit (OKRA00014)

OKRA00014 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00015)

OKRA00015 1 each
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00016)

OKRA00016 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00017)

OKRA00017 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-050 500ml
EUR 144

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-100 2x500ml
EUR 176.4

Bovine DNAse 1 Protein Western blot +ve control

DNASE11-C 100 ul
EUR 343.2

Mouse Leptin protein Western blot +ve control # 1

LEP11-C 100 ul
EUR 343.2

cyclin G2 Western Blot kit (AWBK03032)

AWBK03032 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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Pure-IP Western Blot Detection Kit

PRB-5002 20 blots
EUR 470.4
Description: Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection Kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody.

PBS Buffer Western Blot 10X, pH 7.4

41620013-1 1 L Ask for price

PBS Buffer Western Blot 10X, pH 7.4

41620013-2 2 L Ask for price

PBS Buffer Western Blot 10X, pH 7.4

41620013-3 4 L Ask for price

PBS Buffer Western Blot 10X, pH 7.4

41620013-4 6 L Ask for price

Chemiluminescent Western Blot Kit (OKRA00001)

OKRA00001 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00002)

OKRA00002 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00003)

OKRA00003 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00004)

OKRA00004 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

T-Pro Western Blot Stripping Reagent

JB11-K002 500ml/BT
EUR 213.6

AgileBlotter? Automated Western Blot Processor

ACT-ABH-40 each
EUR 20326.8

Nitrated Mol Wt Std for Western blot

NITT12-C 100 ul
EUR 343.2

Custom siRNA

M1253-10 each
EUR 666

Custom siRNA

M1253-2 each
EUR 373.2

Custom siRNA

M1253-3 each
EUR 405.6

Custom siRNA

M1253-4 each
EUR 438

Custom siRNA

M1253-5 each
EUR 535.2

Custom siRNA

M1253-6 each
EUR 548.4

Custom siRNA

M1253-7 each
EUR 568.8

Custom siRNA

M1253-8 each
EUR 594

Custom siRNA

M1253-9 each
EUR 633.6

Attoglow Western Blot Analysis Kit:Blocking Agent

K3171250-3 40 g
EUR 68

Western Blot Primary Antibody Diluent Buffer

abx090689-100ml 100 ml
EUR 218.4

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-10 20mL (sufficient reagents for 4000 cm2 of membrane)
EUR 200.4

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-25 50mL (sufficient reagents for 10000 cm2 of membrane)
EUR 376.8

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-5 10mL (sufficient reagents for 2000 cm2 of membrane)
EUR 139.2

Western Blot Box - 6 x 4 x 1 1/4in.; 15.2 x 10.2 x 3.2cm

B1200-17 5/pack
EUR 107.63
Description: Western Blot Boxes

TBS High Salt for Western Blot Washing 10X

10530026-1 1 L
EUR 40.37

TBS High Salt for Western Blot Washing 10X

10530026-2 4 L
EUR 111.42

Purified Ram Cyclooxygenase (Cox-1) Western blot +ve control

COX11-C 100 ul
EUR 343.2

Recombinant Human MutY Protein for Western blot

MUTY11-C 100 ul
EUR 343.2

ChonBlock Western Blot Buffer 2 for Goat (PBS)

90691P 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Goat (PBS)

Simple Western: Bringing the Western Blot into the Twenty-First Century

The Western blot is widely used in the study of protein biochemistry, but it is notoriously labor-intensive, and it is limited in its reproducibility and quantification, among many other challenges. By contrast, capillary-based protein separation and immunodetection, known as Simple Western™, overcomes many of the challenges associated with the traditional Western blot, and it is quickly gaining traction as a replacement for traditional Western blot analysis.

The advantages that capillary-based immunoassay offers include ease of use, automation, reproducibility, quantification, and even built-in total protein normalization. In this chapter, we describe protocols for the two basic types of capillary-based immunodetection assays, one by molecular weight separation and the other by charge separation. In both methods, protein samples are separated in the capillary followed seamlessly by immunodetection with chemiluminescent or fluorescent antibodies for highly sensitive and specific detection of target proteins.

Biological Validation of a Novel Process and Product for Quantitating Western Blots

Protein normalization of western blots has relied upon housekeeping proteins which exhibit signal saturation and varied cellular expression level variations. These issues can produce spurious results leading to erroneous conclusions. A superior method to protein normalization using housekeeping proteins is Total Protein Normalization, a method now recognized as the gold standard for quantitative westerns.

Total Protein Normalization requires that all proteins on a membrane be stained or labeled uniformly, imaged, and then analyzed for total protein. It is important that such a normalization process not interfere with typical immunodetection methods, fits within existing western workflows, and exhibits a linear relationship of signal intensity to protein load under all experimental conditions. Here we report that we developed a new reagent enabling Total Protein Normalization, and we demonstrate its superior protein normalization capabilities through analysis of target proteins in different cell backgrounds.

These data illustrate how housekeeping proteins exhibit signal saturation, yield erroneous normalization data, and display sample-to-sample variations averaging 48.2% overall. Signal intensities obtained using our new method show a linear relationship to protein sample load, thus providing accurate protein normalization with an overall average variation of 7.7%.

 

Identification of Protein Carbonyls (PCOs) in Canine Serum by Western Blot Technique and Preliminary Evaluation of PCO Concentration in Dogs With Systemic Inflammation

In people, serum Protein Carbonyls (PCOs) increase during oxidative stress (OS) due to oxidative damage to proteins. OS is often associated with inflammation and especially with sepsis, a condition hard to diagnose in veterinary medicine because reliable markers are lacking. The aim of this study was to assess whether PCOs in canine serum may be detected by antibody-based methods such as Western Blotting (WB), and to preliminarily investigate the possible utility of this marker in dogs with inflammation.

A serum sample oxidized in vitro was used to set up the method; the coefficient of variation obtained by repeated analysis varied from 24 to 36%. In order to assess whether the technique may cover the range of PCOs concentration detectable in routine practice, PCOs were measured in 4 healthy dogs and in 15 with inflammatory diseases, in some cases potentially associated with sepsis, as suggested by the results of other inflammatory markers such as C-Reactive Protein (CRP) and the anti-oxidant enzyme Paraoxonase 1 (PON-1): the concentration of PCOs was low in dogs with normal PON-1 activity,

moderately increased in the majority of dogs with low-normal PON-1 activity, and severely increased in dogs with very low PON-1 activity. In conclusion this study demonstrates that PCOs, may be detected in canine serum, using antibody-based techniques such as WB. The preliminary results in dogs with and without systemic inflammation encourage further studies on the possible role of PCOs as inflammatory markers.

 

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