Identification of early diagnostic antigens in soluble proteins of Trichinella spiralis

Identification of early diagnostic antigens in soluble proteins of Trichinella spiralis

Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Mitochondrial Respiratory Chain Complexes

Cardiac, neuronal and renal tubular epithelial cells are the most metabolically active cells in the body. Their fate depends largely on their mitochondria as the primary energy generating system which participates in the control of apoptosis, cell cycle and metabolism. Thus, mitochondrial dysfunction is a hallmark of many chronic diseases including diabetic nephropathy. A drop in mitochondrial bioenergetics efficiency is often associated with altered expression of respiratory chain complexes. Moreover, recent studies demonstrate that cellular proteins can shuttle to mitochondria and modify their function directly.

Here we illustrate two mitochondria isolation protocols; one is recommended if the purity of the mitochondrial fraction is a priority such as if the mitochondrial localization of a protein has to be validated, the other if a high yield of intact functional mitochondria is required for functional studies and quantitative Western blotting. Next, we provide a detailed protocol for Western blotting of isolated mitochondria and renal cortex either to prove the purity of isolated fractions or to quantify complexes of the mitochondrial respiratory chain. We used this approach to identify classically cell membrane bound angiotensin II receptors in mitochondria and to study the effect of these receptors on mitochondrial function in early stages of diabetic nephropathy.

Identification of early diagnostic antigens in soluble proteins of Trichinella spiralis adult worms by Western blot

Previous studies showed that crude antigens from Trichinella spiralis adult worms (AW) can be recognized by mouse infection sera at 8 days post infection. The aim of this study was to identify the early diagnostic antigenic bands in soluble proteins from T. spiralis AW by Western blot using early infection sera. The affecting factors of adult recovery were firstly observed in this study, and the results showed that the maximum number of adults was collected from small intestine when the female BALB/c mice were orally infected with 4000 ML and sacrificed at 3 days post infection.

The results of Western blot analysis showed that seven protein bands (31, 35.1, 39, 40.6, 41.9, 47 and 50.6 kDa) could be recognized by early infection sera as early as at 8-10 days post infection, and were strongly reacted with mouse infection sera at 11-12 days post infection. Our results suggested that the seven protein bands of T. spiralis AW soluble proteins might be the early expressed antigens during the intestinal stage of Trichinella infection and therefore have potential value for the early diagnosis of trichinellosis.

nwreprosci
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ChonBlock Western Blot Buffer 1 (TBS)
9069T 10 ml
EUR 193.55
Description: ChonBlock Western Blot Buffer 1 (TBS)
TMB Substrate, for Western Blot
F100029 1000 ml
EUR 827
  • Product category: Buffer solution
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.
TMB Substrate, for Western Blot
F129-100 100 ml
EUR 249
  • Product category: Buffer solution
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.
Interferon alpha Western Blot Antibody
20-abx137014
  • EUR 356.00
  • EUR 523.00
  • 0.5 mg
  • 1 mg
  • Shipped within 5-10 working days.
AgileBlotter? Automated Western Blot Processor
ACT-ABH-40
EUR 16939
Hypersensitive Western Blot Chemiluminescent Substrate
CR0001-10 20mL (sufficient reagents for 4000 cm2 of membrane)
EUR 167
Hypersensitive Western Blot Chemiluminescent Substrate
CR0001-25 50mL (sufficient reagents for 10000 cm2 of membrane)
EUR 314
Hypersensitive Western Blot Chemiluminescent Substrate
CR0001-5 10mL (sufficient reagents for 2000 cm2 of membrane)
EUR 116
PDCD4 Western Blot kit (AWBK09045)
AWBK09045 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
ETS1 Western Blot kit (AWBK100604)
AWBK100604 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
ERG Western Blot kit (AWBK100625)
AWBK100625 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
GABRP Western Blot kit (AWBK13034)
AWBK13034 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
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  • Assay info:
  • Sensitivity:
HTR1A Western Blot kit (AWBK13041)
AWBK13041 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
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  • Assay info:
  • Sensitivity:
RAB14 Western Blot kit (AWBK13107)
AWBK13107 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
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  • Assay info:
  • Sensitivity:
TNRC4 Western Blot kit (AWBK31525)
AWBK31525 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
ESRRG Western Blot kit (AWBK31655)
AWBK31655 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
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  • Assay info:
  • Sensitivity:
GLI2 Western Blot kit (AWBK31885)
AWBK31885 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
PAX4 Western Blot kit (AWBK32064)
AWBK32064 10 reactions
EUR 647
Description:
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  • Species reactivity:
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  • Assay info:
  • Sensitivity:
AHR Western Blot kit (AWBK32243)
AWBK32243 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
GLI1 Western Blot kit (AWBK32368)
AWBK32368 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
PAX7 Western Blot kit (AWBK32393)
AWBK32393 10 reactions
EUR 647
Description:
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  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
LHX6 Western Blot kit (AWBK32553)
AWBK32553 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
HIPK2 Western Blot kit (AWBK32586)
AWBK32586 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
PAX7 Western Blot kit (AWBK32742)
AWBK32742 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
ZFP67 Western Blot kit (AWBK32749)
AWBK32749 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
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  • Assay info:
  • Sensitivity:
OLIG2 Western Blot kit (AWBK32753)
AWBK32753 10 reactions
EUR 647
Description:
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  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
ENG Western Blot kit (AWBK33068)
AWBK33068 10 reactions
EUR 647
Description:
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  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
SOX5 Western Blot kit (AWBK33323)
AWBK33323 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
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  • Assay info:
  • Sensitivity:
SOX10 Western Blot kit (AWBK33326)
AWBK33326 10 reactions
EUR 647
Description:
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  • Species reactivity:
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  • Sensitivity:
RPS16 Western Blot kit (AWBK33535)
AWBK33535 10 reactions
EUR 647
Description:
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  • Species reactivity:
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  • Sensitivity:
ATP7A Western Blot kit (AWBK33797)
AWBK33797 10 reactions
EUR 647
Description:
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  • Sensitivity:
A1BG Western Blot kit (AWBK33810)
AWBK33810 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity: Human
  • Application:
  • Assay info:
  • Sensitivity:
NPM1 Western Blot kit (AWBK34094)
AWBK34094 10 reactions
EUR 647
Description:
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  • Species reactivity:
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  • Sensitivity:
LIG4 Western Blot kit (AWBK34122)
AWBK34122 10 reactions
EUR 647
Description:
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  • Species reactivity:
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  • Sensitivity:
RNF2 Western Blot kit (AWBK34290)
AWBK34290 10 reactions
EUR 647
Description:
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  • Species reactivity:
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  • Assay info:
  • Sensitivity:
ZFP36 Western Blot kit (AWBK34385)
AWBK34385 10 reactions
EUR 647
Description:
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  • Species reactivity:
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  • Assay info:
  • Sensitivity:
ARIH2 Western Blot kit (AWBK34418)
AWBK34418 10 reactions
EUR 647
Description:
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  • Species reactivity:
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  • Assay info:
  • Sensitivity:
KEAP1 Western Blot kit (AWBK34728)
AWBK34728 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
CLIC1 Western Blot kit (AWBK35045)
AWBK35045 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
KCNN2 Western Blot kit (AWBK35094)
AWBK35094 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
CLCN3 Western Blot kit (AWBK35504)
AWBK35504 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
P2RX7 Western Blot kit (AWBK35518)
AWBK35518 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
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  • Assay info:
  • Sensitivity:
TRPM3 Western Blot kit (AWBK35609)
AWBK35609 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
PITX2 Western Blot kit (AWBK35634)
AWBK35634 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
ILF2 Western Blot kit (AWBK35731)
AWBK35731 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
ONECUT2 Western Blot kit (AWBK35750)
AWBK35750 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
ZFP42 Western Blot kit (AWBK36244)
AWBK36244 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
ANXA1 Western Blot kit (AWBK36568)
AWBK36568 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
SPP1 Western Blot kit (AWBK36677)
AWBK36677 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
RUNX2 Western Blot kit (AWBK36678)
AWBK36678 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
ZBP1 Western Blot kit (AWBK36711)
AWBK36711 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
Bapx1 Western Blot kit (AWBK36826)
AWBK36826 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
G3BP Western Blot kit (AWBK37713)
AWBK37713 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
TWIST1 Western Blot kit (AWBK37997)
AWBK37997 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
ID4 Western Blot kit (AWBK38058)
AWBK38058 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
SATB1 Western Blot kit (AWBK38208)
AWBK38208 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
EED Western Blot kit (AWBK38384)
AWBK38384 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
TARDBP Western Blot kit (AWBK38941)
AWBK38941 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
TARDBP Western Blot kit (AWBK38942)
AWBK38942 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
BRD7 Western Blot kit (AWBK39018)
AWBK39018 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
JMJD3 Western Blot kit (AWBK40102)
AWBK40102 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
SNRP70 Western Blot kit (AWBK40439)
AWBK40439 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
SSB Western Blot kit (AWBK40462)
AWBK40462 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
STAU1 Western Blot kit (AWBK40537)
AWBK40537 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
PCBP1 Western Blot kit (AWBK40630)
AWBK40630 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
HNRPLL Western Blot kit (AWBK41102)
AWBK41102 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
MSI2 Western Blot kit (AWBK41112)
AWBK41112 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
HNRPA3 Western Blot kit (AWBK41195)
AWBK41195 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
WNT2B Western Blot kit (AWBK41254)
AWBK41254 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
GNB1L Western Blot kit (AWBK41328)
AWBK41328 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
SLC12A1 Western Blot kit (AWBK41388)
AWBK41388 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
TPM1 Western Blot kit (AWBK41392)
AWBK41392 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
CEACAM6 Western Blot kit (AWBK41504)
AWBK41504 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
SLC22A1 Western Blot kit (AWBK41516)
AWBK41516 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
STK11 Western Blot kit (AWBK41743)
AWBK41743 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
OTC Western Blot kit (AWBK41766)
AWBK41766 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
TSHR Western Blot kit (AWBK41870)
AWBK41870 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
RHOBTB1 Western Blot kit (AWBK41883)
AWBK41883 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
KRT13 Western Blot kit (AWBK42031)
AWBK42031 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
IDH3A Western Blot kit (AWBK42237)
AWBK42237 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
SLC1A5 Western Blot kit (AWBK42247)
AWBK42247 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
PBEF1 Western Blot kit (AWBK42255)
AWBK42255 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
FST Western Blot kit (AWBK42287)
AWBK42287 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
SLC22A7 Western Blot kit (AWBK42306)
AWBK42306 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
ASPN Western Blot kit (AWBK42487)
AWBK42487 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
RSAD2 Western Blot kit (AWBK42696)
AWBK42696 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
PANX2 Western Blot kit (AWBK42778)
AWBK42778 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
PANX1 Western Blot kit (AWBK42783)
AWBK42783 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
TGFBI Western Blot kit (AWBK44268)
AWBK44268 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
PTPN2 Western Blot kit (AWBK45362)
AWBK45362 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
RORA Western Blot kit (AWBK45608)
AWBK45608 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
Chemiluminescent Western Blot Kit (OKRA00001)
OKRA00001 1 kit
EUR 570
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:
Chemiluminescent Western Blot Kit (OKRA00002)
OKRA00002 1 kit
EUR 570
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:
Chemiluminescent Western Blot Kit (OKRA00003)
OKRA00003 1 kit
EUR 570
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:
Chemiluminescent Western Blot Kit (OKRA00004)
OKRA00004 1 kit
EUR 570
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:
Colorimetric Western Blot Kit (OKRA00014)
OKRA00014 1 kit
EUR 609
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:
Colorimetric Western Blot Kit (OKRA00015)
OKRA00015 1 each
EUR 609
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:
Colorimetric Western Blot Kit (OKRA00016)
OKRA00016 1 kit
EUR 609
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:
Colorimetric Western Blot Kit (OKRA00017)
OKRA00017 1 kit
EUR 609
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:
Western equine encephalitis PCR kit
PCR-VH166-48R 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Western equine encephalitis
Western equine encephalitis PCR kit
PCR-VH166-96R 100T
EUR 669.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Western equine encephalitis
T-Pro Western Blot Stripping Reagent
JB11-K002 500ml/BT
EUR 178
Western Blot Primary Antibody Diluent Buffer
abx090689-100ml 100 ml
EUR 182
  • Shipped within 5-10 working days.
Attoglow Western Blot Analysis Kit:Blocking Agent
K3171250-3 40 g
EUR 109
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Pure-IP Western Blot Detection Kit
PRB-5002 20 blots
EUR 392
Description: Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection Kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody.
cyclin G2 Western Blot kit (AWBK03032)
AWBK03032 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
Bovine DNAse 1 Protein Western blot +ve control
DNASE11-C 100 ul
EUR 286
Mouse Leptin protein Western blot +ve control # 1
LEP11-C 100 ul
EUR 286
Western equine encephalitis RT PCR kit
RTq-VH166-100R 100T
EUR 776.4
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Western equine encephalitis .
Western equine encephalitis RT PCR kit
RTq-VH166-150R 150T
EUR 877.9
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Western equine encephalitis .
Western equine encephalitis RT PCR kit
RTq-VH166-50R 50T
EUR 631.4
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Western equine encephalitis .
Total Protein Western Blots - Human Adult Normal Tissue, Blot IV, 16 lanes
W1234404 1
EUR 934
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Pre-made Minicircle CMV-GFP-SV40PolyA (positive control)
MN601MC-1 40 ug
EUR 735
  • Category: Minicircle Technology
Pre-made Minicircle CMV-rRFP-SV40PolyA (positive control)
MN602MC-1 40 ug
EUR 735
  • Category: Minicircle Technology
Purified Ram Cyclooxygenase (Cox-1) Western blot +ve control
COX11-C 100 ul
EUR 286
West-Ez Blocking Buffer, 1 % Casein for Western Blot
W3720-050 500ml
EUR 120
West-Ez Blocking Buffer, 1 % Casein for Western Blot
W3720-100 2x500ml
EUR 147
Poly-ADP-ribosylated proteins for Western blot
ADPR11-C 100 ul
EUR 286
Mouse Ceruloplasmin protein control for western blot
CP16-C 100 ul
EUR 286
Rat Ceruloplasmin protein control for western blot
CP17-C 100 ul
EUR 286
Attoglow Western Blot Analysis Kit:Binding Buffer 20x
K3171250-2 120 ml
EUR 143
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Recombinant Human MutY Protein for Western blot
MUTY11-C 100 ul
EUR 286
Nitrated Mol Wt Std for Western blot
NITT12-C 100 ul
EUR 286
SuperBrite? ECL Western Blot Substrate/Detection Kit
K824-50
EUR 191
Western equine encephalitis One-Step PCR kit
Oneq-VH166-100R 100T
EUR 933
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Western equine encephalitis.
Western equine encephalitis One-Step PCR kit
Oneq-VH166-150R 150T
EUR 1060.6
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Western equine encephalitis.
Western equine encephalitis One-Step PCR kit
Oneq-VH166-50R 50T
EUR 751.75
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Western equine encephalitis.
Custom Neg Control siRNA
M1256-1
EUR 278
Custom Pos Control siRNA
M1258-1
EUR 278
Equine IFN beta 1 Recombinant Protein
R02041-1 5ug/vial
EUR 259
Description: IFN beta is a mammalian Type I inferferon, functionig as a regulator of cellular activity by interacting with cell-surface receptors and activating various signaling pathways. IFN beta produces antiviral, antibacterial, and anticancer properties. Equine IFN beta Recombinant Protein is purified IFN beta produced in yeast.
Chicken Serum Albumin protein control for Western blot
ALBC15-C 100 ul
EUR 286
Beta-Galactosidase (E. Coli) Protein for Western Blot
BGAL11-C 100 ul
EUR 286
Recombinant Beta Lactamase protein control for Western blot
BLAC11-C 100 ul
EUR 286
Human C1 Esterase Inhibitor western blot +ve control
C1E11-C 100 ul
EUR 286
Purified Human C1q protein Western blot +ve control
C1Q11-C 100 ul
EUR 286
Human Spleen Ferritin protein Western blot +ve control
FERT11-C 100 ul
EUR 286
Human Liver Ferritin protein Western blot +ve control
FERT12-C 100 ul
EUR 286
Horse Spleen Ferritin protein Western blot +ve control
FERT13-C 100 ul
EUR 286
Western blot recycling stripping buffer, 50 ml (10X)
90101 50 ml
EUR 238
ChonBlock Western Blot Buffer 2 for Goat (PBS)
90691P 10 ml
EUR 121.05
Description: ChonBlock Western Blot Buffer 2 for Goat (PBS)
ChonBlock Western Blot Buffer 2 for Goat (TBS)
90691T 10 ml
EUR 121.05
Description: ChonBlock Western Blot Buffer 2 for Goat (TBS)
ChonBlock Western Blot Buffer 2 for Rabbit (PBS)
90692P 10 ml
EUR 121.05
Description: ChonBlock Western Blot Buffer 2 for Rabbit (PBS)
ChonBlock Western Blot Buffer 2 for Rabbit (TBS)
90692T 10 ml
EUR 121.05
Description: ChonBlock Western Blot Buffer 2 for Rabbit (TBS)
Purified Cholera Toxin protein control for Western Blot
CTOX11-C 100 ul
EUR 286
Human Pancreatic Amylase protein control for Western blot
PAMY13-C 100 ul
EUR 286
Human Leptin protein Western blot +ve control # 2
LEP12-C 100 ul
EUR 286
HRP Western Blot Anti-IgG Antibody Kit (OKRA00006)
OKRA00006 100 ug
EUR 205
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:
Custom Peptide Synthesis order form
Pepord-1 1 Ask for price
Recombinant 1 Envelope glycoprotein 2 (1 E2) Protein control for western blot
BVDE11-C 100 ul
EUR 286
Mouse NOS-II/i-NOS Protein Western Blot +ve Control # 1
iNOS-C 100 ul
EUR 286
Equine CCL3 Recombinant Protei
R00405-1 5ug/vial
EUR 259
Description: Chemokine ligand 3 (CCL3), also known as Macrophage Inflammatory Protein-1 alpha (MIP-1 alpha), is a small cytokine belonging to the CC chemokine family. CCL3 (MIP-1 alpha) is a chemoattractant for several different leukocytes, with varying degrees of potency. Equine CCL3 Recombinant Protein is purified chemokine ligand 3 (CCL3, MIP-1 alpha) produced in yeast.
Equine EPO Recombinant Protein
R00484-1 5ug/vial
EUR 259
Description: Erythropoietin (EPO) is an essential hormone for red blood cell production. Erythropoietin has a range of actions including vasoconstriction-dependent hypertension, stimulating angiogenesis, and inducing proliferation of smooth muscle fibers. Equine Erythropoietin Recombinant Protein is purified Erythropoietin (EPO) produced in yeast.
Equine CXCL9 Recombinant Protein
R01397-1 5ug/vial
EUR 259
Description: CXCL9 (MIG) is a T-cell chemoattractant. Induced by IFN-gamma (IFN-γ), the ELR-negative chemokine CXCL9 (MIG) elicits its effects by binding to the cell surface chemokine receptor CXCR3. Equine CXCL9 Recombinant Protein is purified CXCL9 (MIG) produced in yeast.
Equine CCL11 Recombinant Protein
R01438-1 5ug/vial
EUR 259
Description: Chemokine ligand 11 (CCL11) belongs to the CC chemokine family and is commonly known as Eotaxin-1. CCL11 (Eotaxin-1) selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses. Equine CCL11 Recombinant Protein is purified chemokine ligand 11 (CCL11, Eotaxin-1) produced in yeast.
Recombinant Norovirus 1 (MNV-1) Capsid Protein 1 (VP1) control for Western blot
MNV14-C 100 ul
EUR 286
Purified Mouse serum albumin protein control for Western blot
ALB11-C 100 ul
EUR 286
Purified Human serum albumin protein control for Western blot
ALBH13-C 100 ul
EUR 286
Purified rat serum albumin protein control for Western blot
ALBR12-C 100 ul
EUR 286
Purified Sheep serum albumin protein control for Western blot
ALBSH20-C 100 ul
EUR 286
Human recombinant, purified BMP2 protein Western blot +ve control
BMP21-C 100 ul
EUR 286
Human recombinant, purified BMP3 protein Western blot +ve control
BMP31-C 100 ul
EUR 286
Human recombinant, purified BMP4 protein Western blot +ve control
BMP41-C 100 ul
EUR 286
Human recombinant, purified BMP5 protein Western blot +ve control
BMP51-C 100 ul
EUR 286
Human recombinant, purified BMP6 protein Western blot +ve control
BMP61-C 100 ul
EUR 286
Recombinant human BAFF-R protein control for Western blot
BAFFR11-C 100 ul
EUR 286
Human Adipsin/Factor D protein Western blot +ve control
ADN12-C 100 ul
EUR 286
Human hemoglobin (non-glycated) protein control for western blot
HEMG13-C 100 ul
EUR 286
Single solution TMB substrate (membrane) solution for western blot
80110 100 mls
EUR 225
Western blot Kit for Rabbit Primary Antibodies, Chemilum. Substrate
80200-Rb 1 kit
EUR 651
Western blot Kit for Goat Primary Antibodies, Chemilum. Substrate
80201-Gt 1 kit
EUR 651
Western blot Kit for Mouse Primary Antibodies, Chemilum. Substrate
80202-Mo 1 kit
EUR 651
Western blot Kit for Rat Primary Antibodies, Chemilum. Substrate
80203-Rt 1 kit
EUR 651
Western blot Kit for Chicken Primary Antibodies, Chemilum. Substrate
80204-Ch 1 kit
EUR 651
Western blot Kit for Monkey Primary Antibodies, Chemilum. Substrate
80205-Mk 1 kit
EUR 651
Western blot Kit for Human Primary Antibodies, Chemilum. Substrate
80207-Hu 1 kit
EUR 651
Western blot Kit for Sheep Primary Antibodies; Chemilum. Substrate
80208-Sh 1 kit
EUR 651
Western blot Kit for Pig Primary Antibodies; Chemilum. Substrate
80209-Pg 1 kit
EUR 651
NuGlo ECL Substrate Kit for Western blot, 400 mls
80215 1 kit
EUR 408
WesternOne? Marker Pen, HRP for Western blot chemiluminescent detection
ACT-WP
EUR 144
Human Liver Cathepsin B protein control for Western blot
CTHB11-C 100 ul
EUR 286
Human Liver Cathepsin D protein control for Western blot
CTHD11-C 100 ul
EUR 286
Human Liver Cathepsin G protein control for Western blot
CTHG11-C 100 ul
EUR 286
Human Liver Cathepsin H protein control for Western blot
CTHH11-C 100 ul
EUR 286

Simple Western: Bringing the Western Blot into the Twenty-First Century

The Western blot is widely used in the study of protein biochemistry, but it is notoriously labor-intensive, and it is limited in its reproducibility and quantification, among many other challenges. By contrast, capillary-based protein separation and immunodetection, known as Simple Western™, overcomes many of the challenges associated with the traditional Western blot, and it is quickly gaining traction as a replacement for traditional Western blot analysis.

The advantages that capillary-based immunoassay offers include ease of use, automation, reproducibility, quantification, and even built-in total protein normalization. In this chapter, we describe protocols for the two basic types of capillary-based immunodetection assays, one by molecular weight separation and the other by charge separation. In both methods, protein samples are separated in the capillary followed seamlessly by immunodetection with chemiluminescent or fluorescent antibodies for highly sensitive and specific detection of target proteins.

Biological Validation of a Novel Process and Product for Quantitating Western Blots

Protein normalization of western blots has relied upon housekeeping proteins which exhibit signal saturation and varied cellular expression level variations. These issues can produce spurious results leading to erroneous conclusions. A superior method to protein normalization using housekeeping proteins is Total Protein Normalization, a method now recognized as the gold standard for quantitative westerns.

Total Protein Normalization requires that all proteins on a membrane be stained or labeled uniformly, imaged, and then analyzed for total protein. It is important that such a normalization process not interfere with typical immunodetection methods, fits within existing western workflows, and exhibits a linear relationship of signal intensity to protein load under all experimental conditions. Here we report that we developed a new reagent enabling Total Protein Normalization, and we demonstrate its superior protein normalization capabilities through analysis of target proteins in different cell backgrounds.

These data illustrate how housekeeping proteins exhibit signal saturation, yield erroneous normalization data, and display sample-to-sample variations averaging 48.2% overall. Signal intensities obtained using our new method show a linear relationship to protein sample load, thus providing accurate protein normalization with an overall average variation of 7.7%.

 

Identification of Protein Carbonyls (PCOs) in Canine Serum by Western Blot Technique and Preliminary Evaluation of PCO Concentration in Dogs With Systemic Inflammation

In people, serum Protein Carbonyls (PCOs) increase during oxidative stress (OS) due to oxidative damage to proteins. OS is often associated with inflammation and especially with sepsis, a condition hard to diagnose in veterinary medicine because reliable markers are lacking. The aim of this study was to assess whether PCOs in canine serum may be detected by antibody-based methods such as Western Blotting (WB), and to preliminarily investigate the possible utility of this marker in dogs with inflammation.

A serum sample oxidized in vitro was used to set up the method; the coefficient of variation obtained by repeated analysis varied from 24 to 36%. In order to assess whether the technique may cover the range of PCOs concentration detectable in routine practice, PCOs were measured in 4 healthy dogs and in 15 with inflammatory diseases, in some cases potentially associated with sepsis, as suggested by the results of other inflammatory markers such as C-Reactive Protein (CRP) and the anti-oxidant enzyme Paraoxonase 1 (PON-1): the concentration of PCOs was low in dogs with normal PON-1 activity,

moderately increased in the majority of dogs with low-normal PON-1 activity, and severely increased in dogs with very low PON-1 activity. In conclusion this study demonstrates that PCOs, may be detected in canine serum, using antibody-based techniques such as WB. The preliminary results in dogs with and without systemic inflammation encourage further studies on the possible role of PCOs as inflammatory markers.

 

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