Near misdiagnosis of acute HIV-infection with ELISA-Western Blot scheme

Near misdiagnosis of acute HIV-infection with ELISA-Western Blot scheme

Protein Quantification Utilizing the “Fast Western Blot” Strategy

  • For the quantification of sure proteins of curiosity inside a fancy pattern, Western blot evaluation is probably the most broadly used methodology. It allows detection of a goal protein based mostly on using particular antibodies. Nevertheless, the entire process is commonly very time-consuming. However, with the event of quick blotting techniques and additional growth of immunostaining strategies, a discount of the processing time may be achieved.
  • Main challenges for the dependable protein quantification by Western blotting are satisfactory knowledge normalization and secure protein detection. Normally, normalization of the goal protein sign is carried out based mostly on housekeeping proteins (e.g., glyceraldehyde 3-phosphate dehydrogenase, ß-actin) with the belief that these proteins are expressed constitutively on the similar stage throughout experiments. Nevertheless, a number of research have already proven that this isn’t all the time the case making this method suboptimal.
  • One other technique makes use of complete protein normalization the place the abundance of the goal protein is expounded to the entire protein quantity in every lane. This method is impartial of a single loading management, and precision of quantification and reliability is elevated. For Western blotting a number of detection strategies can be found, e.g., colorimetric, chemiluminescent, radioactive, fluorescent detection. Standard colorimetric staining tends to endure from low sensitivity, restricted dynamic vary, and low reproducibility. Chemiluminescence-based strategies are easy, however the detected sign doesn’t linearly correlate to protein abundance (from protein quantities >5μg) and have a comparatively slim dynamic vary. Radioactivity is dangerous to well being.
  • To beat these limitations, stain-free strategies had been developed permitting the mixture of fluorescent requirements and a stain-free fluorescence-based visualization of complete protein in gels and after switch to the membrane. Right here, we current a speedy Western blot protocol, which mixes quick blotting utilizing the iBlot system and quick immunostaining using ReadyTector®all-in-one resolution with the Sensible Protein Layers (SPL) method.

 

Close to misdiagnosis of acute HIV-infection with ELISA-Western Blot scheme: Time for mindset change

Some HIV-infection diagnostic tips and well being care suppliers nonetheless depend on the ELISA-Western blot diagnostic algorithm. We current a close to misdiagnosis case with discordant check outcomes and a scarcity of correct counseling. We level out the necessity for an assertive replace of well being care suppliers on diagnostic HIV-tests.

Preclinical Western Blot within the Period of Digital Transformation and Reproducible Analysis, an Jap Perspective

The present analysis is an interdisciplinary endeavor to develop a essential software in preclinical protein research of illnesses or problems via western blotting. Within the period of digital transformation and open entry rules, an interactive cloud-based database referred to as East-West Blot ( https://rancs-lab.shinyapps.io/WesternBlots ) is designed and developed. The net interactive subject-specific database constructed on the R shiny platform facilitates a scientific literature search on the precise subject material, right here set to western blot research of protein regulation within the preclinical mannequin of TBI.

The software summarizes the present publicly obtainable information via an information visualization approach and quick access to the essential knowledge components and hyperlinks to the examine itself. The applying compiled a relational database of PubMed-indexed western blot research labeled beneath HHS public entry, reporting downstream protein rules offered by fluid percussion damage mannequin of traumatic mind damage.

The guarantees of the developed software embrace progressing towards implementing the rules of 3Rs (alternative, discount, and refinement) for humane experiments, cultivating the conditions of reproducible analysis when it comes to reporting traits, paving the methods for a extra collaborative experimental design in fundamental science, and rendering an up-to-date and summarized perspective of present publicly obtainable information.

nwreprosci
nwreprosci

Antigen-Antibody Pen For Chicken Primary antibodies

PEN-C5 1
EUR 242.4

Antigen-Antibody Pen For Goat Primary antibodies

PEN-G3 1
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Antigen-Antibody Pen For Human Primary antibodies

PEN-H7 1
EUR 242.4

Antigen-Antibody Pen For Mouse Primary antibodies

PEN-M2 1
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PEN-R1 1
EUR 242.4

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PEN-R10 1
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PEN-S4 1
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PEN-T8 1
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PEN-P6 1
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Antigen-Antibody Pen For Biotin-tagged antibodies (all species)

PEN-B9 1
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WHATMAN(TM) MEMBRANE MARKING PEN 10/PK

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Pen m2

LF-P0523 0.1 mg
EUR 420
Description: Pen m2 protein

Pen m2

LF-P0523A 0.5 mg
EUR 1496.4
Description: Pen m2 protein

Pen m2

LF-P0523B 1 mg
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Description: Pen m2 protein

PAP Pen

LP0001 1 ea.
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PAP Pen-Mini

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H-Pen-OH

F-2515.0005 5.0g
EUR 181.2
Description: Sum Formula: C5H11NO2S; CAS# [1113-41-3]

H-Pen-OH

F-2515.0025 25.0g
EUR 588
Description: Sum Formula: C5H11NO2S; CAS# [1113-41-3]

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White Marker Pen

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Boc-Pen(pMeBzl)-OH

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Boc-Pen(pMeBzl)-OH

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Fmoc-Pen(Acm)-OH

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Boc-Pen(Trt)-OH

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Boc-Pen(Trt)-OH

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Fmoc-Pen(Trt)-OH

5-05189 1g Ask for price

Fmoc-Pen(Trt)-OH

5-05190 5g Ask for price

Boc-Pen(pMeBzl)-OH.DCHA

A6663-1000 1 g
EUR 512.4
Description: Boc-Pen(pMeBzl)-OH.DCHA

Boc-Pen(pMeBzl)-OH.DCHA

A6663-5000 5 g
EUR 1688.4
Description: Boc-Pen(pMeBzl)-OH.DCHA

Boc-Pen(Mob)-OH

A-2900.0001 1.0g
EUR 181.2
Description: Sum Formula: C18H27NO5S; CAS# [120944-75-4]

Boc-Pen(Mob)-OH

A-2900.0005 5.0g
EUR 631.2
Description: Sum Formula: C18H27NO5S; CAS# [120944-75-4]

Boc-Pen(Trt)-OH

A-3550.0001 1.0g
EUR 370.8
Description: Sum Formula: C29H33NO4S; CAS# [135592-13-1]

Boc-Pen(Trt)-OH

A-3550.0005 5.0g
EUR 1371.6
Description: Sum Formula: C29H33NO4S; CAS# [135592-13-1]

Boc-Pen(NPys)-OH

A-3650.0001 1.0g
EUR 457.2
Description: Sum Formula: C15H21N3O6S2; CAS# [250375-03-2]

Boc-Pen(NPys)-OH

A-3650.0005 5.0g
EUR 1705.2
Description: Sum Formula: C15H21N3O6S2; CAS# [250375-03-2]

Fmoc-Pen(Acm)-OH

B-1885.0001 1.0g
EUR 327.6
Description: Sum Formula: C23H26N2O5S; CAS# [201531-76-2]

Fmoc-Pen(Acm)-OH

B-1885.0005 5.0g
EUR 1197.6
Description: Sum Formula: C23H26N2O5S; CAS# [201531-76-2]

Fmoc-Pen(Trt)-OH

B-2315.0001 1.0g
EUR 340.8
Description: Sum Formula: C39H35NO4S; CAS# [201531-88-6]

Fmoc-Pen(Trt)-OH

B-2315.0005 5.0g
EUR 1255.2
Description: Sum Formula: C39H35NO4S; CAS# [201531-88-6]

Fmoc-Pen(Trt)-OH

B-2315.0025 25.0g
EUR 4911.6
Description: Sum Formula: C39H35NO4S; CAS# [201531-88-6]

H-D-Pen-OH

F-4235.0005 5.0g
EUR 181.2
Description: Sum Formula: C5H11NO2S; CAS# [52-67-5]

H-D-Pen-OH

F-4235.0025 25.0g
EUR 618
Description: Sum Formula: C5H11NO2S; CAS# [52-67-5]

Lab Marker Pen Blue

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Pen-Type Digital Thermometer

THE1013 EACH
EUR 8.55

Pen Type Infrared Thermometer

THE5211 EACH
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WB 4101 hydrochloride

B6515-25 25 mg
EUR 290.4

WB Stripping Buffer

AR0153 100mL (for 10 assays for an 5 × 8.5cm blot)
EUR 133.2

HPSE-WB Recombinant Protein Human Heparanase-1 WB Control

PROTQ9Y251 Regular: 200ng
EUR 1324.8
Description: Recombinant Heparanase protein HPA1 is produced in CHO cells.;The protein is purified by several orthogonal chromatography steps.

Boc-D-Pen(Acm)-OH

5-05193 5g Ask for price

Boc-D-Pen(Acm)-OH

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Boc-D-Pen(Trt)-OH

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Boc-D-Pen(Trt)-OH

5-05198 10g Ask for price

Fmoc-D-Pen(Acm)-OH

5-05199 1g Ask for price

Fmoc-D-Pen(Acm)-OH

5-05200 5g Ask for price

Fmoc-D-Pen(Trt)-OH

5-05201 10g Ask for price

Fmoc-D-Pen(Trt)-OH

5-05202 50g Ask for price

Ac-DL-Pen(Acm)-OH

5-05203 5g Ask for price

Ac-DL-Pen(Acm)-OH

5-05204 25g Ask for price

Mini Super HT PAP Pen

22005 1EA
EUR 171.6
Description: Minimum order quantity: 1 unit of 1EA

Boc-D-Pen(Trt)-OH

A-3555.0001 1.0g
EUR 204
Description: Sum Formula: C29H33NO4S; CAS# [135592-14-2]

Boc-D-Pen(Trt)-OH

A-3555.0005 5.0g
EUR 704.4
Description: Sum Formula: C29H33NO4S; CAS# [135592-14-2]

Boc-D-Pen(NPys)-OH

A-3655.0001 1.0g
EUR 457.2
Description: Sum Formula: C15H21N3O6S2; CAS# [153815-23-7]

Boc-D-Pen(NPys)-OH

A-3655.0005 5.0g
EUR 1705.2
Description: Sum Formula: C15H21N3O6S2; CAS# [153815-23-7]

Fmoc-D-Pen(Acm)-OH

B-1915.0001 1.0g
EUR 327.6
Description: Sum Formula: C23H26N2O5S; CAS# [201531-77-3]

Fmoc-D-Pen(Acm)-OH

B-1915.0005 5.0g
EUR 1197.6
Description: Sum Formula: C23H26N2O5S; CAS# [201531-77-3]

Fmoc-D-Pen(Trt)-OH

B-2320.0001 1.0g
EUR 340.8
Description: Sum Formula: C39H35NO4S; CAS# [201532-01-6]

Fmoc-D-Pen(Trt)-OH

B-2320.0005 5.0g
EUR 1255.2
Description: Sum Formula: C39H35NO4S; CAS# [201532-01-6]

Fmoc-D-Pen(Trt)-OH

B-2320.0025 25.0g
EUR 4911.6
Description: Sum Formula: C39H35NO4S; CAS# [201532-01-6]

H-D-Pen(Trt)-OH

F-3070.0001 1.0g
EUR 370.8
Description: Sum Formula: C24H25NO2S; CAS# [150025-01-7]

H-D-Pen(Trt)-OH

F-3070.0005 5.0g
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Description: Sum Formula: C24H25NO2S; CAS# [150025-01-7]

Human Penguin(PEN)ELISA Kit

QY-E04702 96T
EUR 433.2

Marker Pen Artline 70 Black

LAB3150 PK12
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Marker Pen Frost Resistant Black

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Greiner Marker Pen FINE black

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Sharpie Dual-Tip Pen Set

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PAP PEN FOR IMMUNO STAINING

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WB / Antibody Stripping Buffer

20-abx090656
  • EUR 226.80
  • EUR 276.00
  • 100 ml
  • 200 ml

WB / Antibody Stripping Buffer

abx090671-200ml 200 ml
EUR 226.8

WB Developing Fixing Kit

AR0132 1kit (Enough for 10 assays)
EUR 90

CHO HCP WB kit

F060 1 kit (application dependant amount of reactions)
EUR 1003.2
Description: CHO HCP WB kit by Cygnus Technologies is available in Europe via Gentaur.

S.cerevisiae HCP WB kit

F130 1 kit (application dependant amount of reactions)
EUR 1003.2
Description: S.cerevisiae HCP WB kit by Cygnus Technologies is available in Europe via Gentaur.

A549 HCP WB kit

F235 1 kit (application dependant amount of reactions)
EUR 1003.2
Description: A549 HCP WB kit by Cygnus Technologies is available in Europe via Gentaur.

E.coli HCP WB kit

F415 1 kit (application dependant amount of reactions)
EUR 1003.2
Description: E.coli HCP WB kit by Cygnus Technologies is available in Europe via Gentaur.

Topoisomerase I WB Marker

TG2011-4 20 units
EUR 523.2

101Bio WB Stripping Solution

P5W3 - Ask for price

101Bio WB Blocking Solution

P5W8 - Ask for price

Block

H5000-WB 1 PC
EUR 5146.62

Wash buffer concentrate (100X) for ELISA, 10 ml

WB-100 10 ml
EUR 123.6

Wash buffer concentrate (100X) for ELISA

WB-150 50 ml
EUR 139.2

TrueBlack WB Blocking Buffer Kit

23013 1kit
EUR 416.4
Description: Minimum order quantity: 1 unit of 1kit

TrueBlack WB Blocking Buffer Kit

23013-T 1kit
EUR 150
Description: Minimum order quantity: 1 unit of 1kit

WB Developing and Fixing Kit

20-abx090662
  • EUR 226.80
  • EUR 276.00
  • 100 ml
  • 500 ml

WB Developing and Fixing Kit

abx090672-500ml 500 ml
EUR 226.8

CHO-CM HCP WB kit

CM060 1 kit (application dependant amount of reactions)
EUR 950.4
Description: CHO-CM HCP WB kit by Cygnus Technologies is available in Europe via Gentaur.

WB ECM kit (Mouse IgG)

LF-QC5001 1 kit
EUR 303.6
Description: Our Enhanced Chemiluminescent Kit is an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Powered by our proprietary and easy to use substrates, a unique blocking agent, highly specific secondary antibodies, and carefully optimized protocols, this kit offers the highest signal to noise ratio available and yields clean results, blot after blot.

WB ECM kit (Rabbit IgG)

LF-QC5002 1 kit
EUR 303.6
Description: Our Enhanced Chemiluminescent Kit is an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Powered by our proprietary and easy to use substrates, a unique blocking agent, highly specific secondary antibodies, and carefully optimized protocols, this kit offers the highest signal to noise ratio available and yields clean results, blot after blot.

WB ECM kit (Goat IgG)

LF-QC5003 1 kit
EUR 303.6
Description: Our Enhanced Chemiluminescent Kit is an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Powered by our proprietary and easy to use substrates, a unique blocking agent, highly specific secondary antibodies, and carefully optimized protocols, this kit offers the highest signal to noise ratio available and yields clean results, blot after blot.

WB ECM kit (Rat IgG)

LF-QC5004 1 kit
EUR 303.6
Description: Our Enhanced Chemiluminescent Kit is an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Powered by our proprietary and easy to use substrates, a unique blocking agent, highly specific secondary antibodies, and carefully optimized protocols, this kit offers the highest signal to noise ratio available and yields clean results, blot after blot.

WB ECM kit (Mouse IgM)

LF-QC5005 1 kit
EUR 303.6
Description: Our Enhanced Chemiluminescent Kit is an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Powered by our proprietary and easy to use substrates, a unique blocking agent, highly specific secondary antibodies, and carefully optimized protocols, this kit offers the highest signal to noise ratio available and yields clean results, blot after blot.

Pichia pastoris HCP WB kit

F145 1 kit (application dependant amount of reactions)
EUR 1003.2
Description: Pichia pastoris HCP WB kit by Cygnus Technologies is available in Europe via Gentaur.

SP2/0 HCP WB kit

F185 1 kit (application dependant amount of reactions)
EUR 1003.2
Description: SP2/0 HCP WB kit by Cygnus Technologies is available in Europe via Gentaur.

NS/0 HCP WB kit

F225 1 kit (application dependant amount of reactions)
EUR 1003.2
Description: NS/0 HCP WB kit by Cygnus Technologies is available in Europe via Gentaur.

ECL SELECT WB DETECTION REAGENT

GERPN2235 EACH
EUR 328.32

Polyclonal PSENEN / PEN-2 Antibody (N-Terminus)

APR13001G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PSENEN / PEN-2 (N-Terminus). This antibody is tested and proven to work in the following applications:

Ac-Pen-Arg-Gly-Asp-Cys-OH

H-1614.0001 1.0mg
EUR 254.4
Description: Sum Formula: C22H36N8O9S2; CAS# [135702-36-2]

Ac-Pen-Arg-Gly-Asp-Cys-OH

H-1614.0005 5.0mg
EUR 907.2
Description: Sum Formula: C22H36N8O9S2; CAS# [135702-36-2]

Comark Pen Type Thermometer -50 to +150C

THE5018 EACH
EUR 45.37

Total Goat Milk Proteins WB kit

F245 1 kit (application dependant amount of reactions)
EUR 1003.2
Description: Total Goat Milk Proteins WB kit by Cygnus Technologies is available in Europe via Gentaur.

Purified bovineTransferrin Protein WB +ve Control

TF19-C 100 ul
EUR 343.2

Bovine Ubiquitin protein WB +Ve control

UBQ11-C 100 ul
EUR 343.2

Purified Bovine nRNP WB +ve control

RNP11-C 100 ul
EUR 343.2

Ecl Select Wb Detection Reagent Kit

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EUR 315.78

SHARPIE(R) DUAL TIP PEN SET ASSORTED C

HS15094 PK8
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Lab Marker Fine Tip Pen Ipa Ink Resistant

PEN40 PK10
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Pen Type Thermometer with Alarm -50 to +300C

THE1508 EACH
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PAP PEN FOR IMMUNOSTAINING 2 MM TIP WID

Z672548-1EA EACH
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Cell Lysis Buffer for WB and IP

abx090622-100ml 100 ml
EUR 260.4

Human Apolipoprotein B protein control for WB

APOB21-C 100 ul
EUR 343.2

Rec. Human CD21 protein control for WB

CD21-C 100 ul
EUR 343.2

Purified Human ceruloplasmin (Cp) WB +ve control

CP11-C 100 ul
EUR 343.2

Recombinant Mouse Neprilysin protein WB +ve control

NEP11-C 100 ul
EUR 343.2

Recombinant Human Neprilysin protein WB +ve control

NEP12-C 100 ul
EUR 343.2

WB DAB Chromogenic Kit (Mouse IgG)-Yellow

LF-QC5006 1 kit
EUR 289.2
Description: DAB is the chromogenic substrate of peroxidase. The reaction product is a brown precipitate insoluble in water, dimethylbenzene or alcohol, which makes DAB suitable for color development reaction in western blotting. This Western Blotting DAB Chromogenic kit provides an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Our proprietary polymeric labeling of peroxidase conjugated secondary antibodies offers superior sensitivity in western blotting, significant increase in the detection range, high signal‐to‐noise ratio, and lower primary antibodies consumption. Powered by our proprietary polymeric labeling technique, DAB substrates, a unique blocking agent, highly specific secondary antibodies and carefully optimized protocols, this kit yields cleanest results, blot after blot.

WB DAB Chromogenic Kit (Goat IgG)-Yellow

LF-QC5007 1 kit
EUR 289.2
Description: DAB is the chromogenic substrate of peroxidase. The reaction product is a brown precipitate insoluble in water, dimethylbenzene or alcohol, which makes DAB suitable for color development reaction in western blotting. This Western Blotting DAB Chromogenic kit provides an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Our proprietary polymeric labeling of peroxidase conjugated secondary antibodies offers superior sensitivity in western blotting, significant increase in the detection range, high signal‐to‐noise ratio, and lower primary antibodies consumption. Powered by our proprietary polymeric labeling technique, DAB substrates, a unique blocking agent, highly specific secondary antibodies and carefully optimized protocols, this kit yields cleanest results, blot after blot.

WB DAB Chromogenic Kit (Rabbit IgG)-Yellow

LF-QC5008 1 kit
EUR 289.2
Description: DAB is the chromogenic substrate of peroxidase. The reaction product is a brown precipitate insoluble in water, dimethylbenzene or alcohol, which makes DAB suitable for color development reaction in western blotting. This Western Blotting DAB Chromogenic kit provides an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Our proprietary polymeric labeling of peroxidase conjugated secondary antibodies offers superior sensitivity in western blotting, significant increase in the detection range, high signal‐to‐noise ratio, and lower primary antibodies consumption. Powered by our proprietary polymeric labeling technique, DAB substrates, a unique blocking agent, highly specific secondary antibodies and carefully optimized protocols, this kit yields cleanest results, blot after blot.

WB DAB Chromogenic Kit (Rat IgG)-Yellow

LF-QC5009 1 kit
EUR 289.2
Description: DAB is the chromogenic substrate of peroxidase. The reaction product is a brown precipitate insoluble in water, dimethylbenzene or alcohol, which makes DAB suitable for color development reaction in western blotting. This Western Blotting DAB Chromogenic kit provides an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Our proprietary polymeric labeling of peroxidase conjugated secondary antibodies offers superior sensitivity in western blotting, significant increase in the detection range, high signal‐to‐noise ratio, and lower primary antibodies consumption. Powered by our proprietary polymeric labeling technique, DAB substrates, a unique blocking agent, highly specific secondary antibodies and carefully optimized protocols, this kit yields cleanest results, blot after blot.

WB DAB Chromogenic Kit (Mouse IgG)-Blue

LF-QC5010 1 kit
EUR 289.2
Description: DAB is the chromogenic substrate of peroxidase. The reaction product is a brown precipitate insoluble in water, dimethylbenzene or alcohol, which makes DAB suitable for color development reaction in western blotting. This Western Blotting DAB Chromogenic kit provides an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Our proprietary polymeric labeling of peroxidase conjugated secondary antibodies offers superior sensitivity in western blotting, significant increase in the detection range, high signal‐to‐noise ratio, and lower primary antibodies consumption. Powered by our proprietary polymeric labeling technique, DAB substrates, a unique blocking agent, highly specific secondary antibodies and carefully optimized protocols, this kit yields cleanest results, blot after blot.

WB DAB Chromogenic Kit (Rabbit IgG)-Blue

LF-QC5011 1 kit
EUR 289.2
Description: DAB is the chromogenic substrate of peroxidase. The reaction product is a brown precipitate insoluble in water, dimethylbenzene or alcohol, which makes DAB suitable for color development reaction in western blotting. This Western Blotting DAB Chromogenic kit provides an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Our proprietary polymeric labeling of peroxidase conjugated secondary antibodies offers superior sensitivity in western blotting, significant increase in the detection range, high signal‐to‐noise ratio, and lower primary antibodies consumption. Powered by our proprietary polymeric labeling technique, DAB substrates, a unique blocking agent, highly specific secondary antibodies and carefully optimized protocols, this kit yields cleanest results, blot after blot.

Haptoglobin Human Plasma protein control for WB

HGLB18-C 100 ul
EUR 343.2

Purified Flagellin (salmonella) protein control for WB

FLGN17-C 100 ul
EUR 343.2

Human Heart Ferritin WB +ve protein control

FERT16-C 100 ul
EUR 343.2

Dedication of Caspase Activation by Western Blot

Apoptosis is a sort of programmed cell loss of life induced by a cascade of biochemical occasions, which ends up in distinct morphological adjustments characterised by cell shrinkage, membrane blebbing, chromatin condensation, and DNA fragmentation. Apoptosis is executed by a category of cysteine proteases referred to as caspases. Caspases are synthesized as inactive pro-caspases and activated by a sequence of cleavage reactions. Lively caspases cleave mobile substrates and are thus the principle effectors of the apoptotic cell loss of life pathway. Detection of caspase cleavage by western blot evaluation is a standard methodology to exhibit the induction of apoptosis.

Within the context of apoptosis, the right evaluation of western blot outcomes relies on the understanding of the mechanisms and outcomes of caspase processing throughout the course of its activation. On this chapter, we describe the step-by-step methodology within the western blot evaluation of caspase cleavage throughout apoptosis. We element protocols for protein extraction, quantitation, casting, and working gel electrophoresis and western blot evaluation of caspase -Eight and caspase -9 activation. The described strategies may be utilized to any explicit protein of curiosity.

Spreader-CDR system for environment friendly, reproducible and frugal western blot

Environment friendly antibody incubation is an important step for profitable western blot. Throughout the incubation, a skinny antibody-depleted layer is created across the blotting membrane which limits antibody binding. Though the traditional batch shaking methodology is ineffective towards it, this layer may be simply disrupted by cyclic draining and replenishing (CDR) of the antibody resolution throughout membrane incubation. Beforehand, we launched a closed and rotating cylindrical chamber as a software to implement CDR for western blots (rCDR). A brand new open bucket-style chamber was devised for simpler operation and the potential of course of automation.

As an alternative of rotation as in rCDR, rocking it backwards and forwards achieved the CDR antibody incubation (R-CDR). The chamber was then geared up with a spreader-rod to facilitate the uniform motion of the antibody resolution throughout the membrane floor. Therefore it was named spreader CDR (S-CDR). In comparison with the batch incubation methodology, each the S-CDR and R-CDR gadgets produced considerably enhanced alerts and developed quicker outcomes. There have been a number of further advantages of utilizing the spreader-rod, which included uniform antibody binding throughout the membrane, diminished utilization of antibodies and the power to recuperate outcomes even from mishandled, creased membranes. The S-CDR machine ensures higher blots and may be simply applied in current western blot protocols. This text is protected by copyright. All rights reserved.

Western Blot Kit

20-abx098123
  • EUR 276.00
  • EUR 343.20
  • 100 ml
  • 200 ml

Western Blot Enhancing Kit

K3172120 1200 cm2
EUR 171.6
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Western Blot Enhancing Kit

K3172250 2500 cm2
EUR 225.6
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Custom siRNA

M1253-2 each
EUR 373.2

Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)

RAT-5 1
EUR 1365.6

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-10 20mL (sufficient reagents for 4000 cm2 of membrane)
EUR 200.4

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-25 50mL (sufficient reagents for 10000 cm2 of membrane)
EUR 376.8

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-5 10mL (sufficient reagents for 2000 cm2 of membrane)
EUR 139.2

TMB Substrate, for Western Blot

F100029 1000 ml
EUR 992.4
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

TMB Substrate, for Western Blot

F129-100 100 ml
EUR 298.8
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

Interferon alpha Western Blot Antibody

20-abx137014
  • EUR 427.20
  • EUR 627.60
  • 0.5 mg
  • 1 mg

PDCD4 Western Blot kit (AWBK09045)

AWBK09045 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ETS1 Western Blot kit (AWBK100604)

AWBK100604 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ERG Western Blot kit (AWBK100625)

AWBK100625 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

GABRP Western Blot kit (AWBK13034)

AWBK13034 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

HTR1A Western Blot kit (AWBK13041)

AWBK13041 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RAB14 Western Blot kit (AWBK13107)

AWBK13107 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TNRC4 Western Blot kit (AWBK31525)

AWBK31525 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ESRRG Western Blot kit (AWBK31655)

AWBK31655 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

GLI2 Western Blot kit (AWBK31885)

AWBK31885 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PAX4 Western Blot kit (AWBK32064)

AWBK32064 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

AHR Western Blot kit (AWBK32243)

AWBK32243 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

GLI1 Western Blot kit (AWBK32368)

AWBK32368 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PAX7 Western Blot kit (AWBK32393)

AWBK32393 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

LHX6 Western Blot kit (AWBK32553)

AWBK32553 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

HIPK2 Western Blot kit (AWBK32586)

AWBK32586 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PAX7 Western Blot kit (AWBK32742)

AWBK32742 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ZFP67 Western Blot kit (AWBK32749)

AWBK32749 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

OLIG2 Western Blot kit (AWBK32753)

AWBK32753 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ENG Western Blot kit (AWBK33068)

AWBK33068 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SOX5 Western Blot kit (AWBK33323)

AWBK33323 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SOX10 Western Blot kit (AWBK33326)

AWBK33326 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RPS16 Western Blot kit (AWBK33535)

AWBK33535 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ATP7A Western Blot kit (AWBK33797)

AWBK33797 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

A1BG Western Blot kit (AWBK33810)

AWBK33810 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity: Human
  • Application:
  • Assay info:
  • Sensitivity:

NPM1 Western Blot kit (AWBK34094)

AWBK34094 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

LIG4 Western Blot kit (AWBK34122)

AWBK34122 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RNF2 Western Blot kit (AWBK34290)

AWBK34290 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ZFP36 Western Blot kit (AWBK34385)

AWBK34385 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ARIH2 Western Blot kit (AWBK34418)

AWBK34418 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

KEAP1 Western Blot kit (AWBK34728)

AWBK34728 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

CLIC1 Western Blot kit (AWBK35045)

AWBK35045 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

KCNN2 Western Blot kit (AWBK35094)

AWBK35094 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

CLCN3 Western Blot kit (AWBK35504)

AWBK35504 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

P2RX7 Western Blot kit (AWBK35518)

AWBK35518 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TRPM3 Western Blot kit (AWBK35609)

AWBK35609 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PITX2 Western Blot kit (AWBK35634)

AWBK35634 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ILF2 Western Blot kit (AWBK35731)

AWBK35731 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ONECUT2 Western Blot kit (AWBK35750)

AWBK35750 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ZFP42 Western Blot kit (AWBK36244)

AWBK36244 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ANXA1 Western Blot kit (AWBK36568)

AWBK36568 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SPP1 Western Blot kit (AWBK36677)

AWBK36677 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RUNX2 Western Blot kit (AWBK36678)

AWBK36678 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ZBP1 Western Blot kit (AWBK36711)

AWBK36711 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

Bapx1 Western Blot kit (AWBK36826)

AWBK36826 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

G3BP Western Blot kit (AWBK37713)

AWBK37713 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TWIST1 Western Blot kit (AWBK37997)

AWBK37997 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ID4 Western Blot kit (AWBK38058)

AWBK38058 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SATB1 Western Blot kit (AWBK38208)

AWBK38208 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

EED Western Blot kit (AWBK38384)

AWBK38384 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TARDBP Western Blot kit (AWBK38941)

AWBK38941 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TARDBP Western Blot kit (AWBK38942)

AWBK38942 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

BRD7 Western Blot kit (AWBK39018)

AWBK39018 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

JMJD3 Western Blot kit (AWBK40102)

AWBK40102 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SNRP70 Western Blot kit (AWBK40439)

AWBK40439 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SSB Western Blot kit (AWBK40462)

AWBK40462 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

STAU1 Western Blot kit (AWBK40537)

AWBK40537 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PCBP1 Western Blot kit (AWBK40630)

AWBK40630 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

HNRPLL Western Blot kit (AWBK41102)

AWBK41102 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

MSI2 Western Blot kit (AWBK41112)

AWBK41112 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

HNRPA3 Western Blot kit (AWBK41195)

AWBK41195 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

WNT2B Western Blot kit (AWBK41254)

AWBK41254 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

GNB1L Western Blot kit (AWBK41328)

AWBK41328 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC12A1 Western Blot kit (AWBK41388)

AWBK41388 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TPM1 Western Blot kit (AWBK41392)

AWBK41392 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

CEACAM6 Western Blot kit (AWBK41504)

AWBK41504 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC22A1 Western Blot kit (AWBK41516)

AWBK41516 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

STK11 Western Blot kit (AWBK41743)

AWBK41743 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

OTC Western Blot kit (AWBK41766)

AWBK41766 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TSHR Western Blot kit (AWBK41870)

AWBK41870 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RHOBTB1 Western Blot kit (AWBK41883)

AWBK41883 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

KRT13 Western Blot kit (AWBK42031)

AWBK42031 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

IDH3A Western Blot kit (AWBK42237)

AWBK42237 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC1A5 Western Blot kit (AWBK42247)

AWBK42247 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PBEF1 Western Blot kit (AWBK42255)

AWBK42255 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

FST Western Blot kit (AWBK42287)

AWBK42287 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC22A7 Western Blot kit (AWBK42306)

AWBK42306 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ASPN Western Blot kit (AWBK42487)

AWBK42487 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RSAD2 Western Blot kit (AWBK42696)

AWBK42696 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PANX2 Western Blot kit (AWBK42778)

AWBK42778 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PANX1 Western Blot kit (AWBK42783)

AWBK42783 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TGFBI Western Blot kit (AWBK44268)

AWBK44268 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PTPN2 Western Blot kit (AWBK45362)

AWBK45362 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RORA Western Blot kit (AWBK45608)

AWBK45608 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00001)

OKRA00001 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00002)

OKRA00002 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00003)

OKRA00003 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00004)

OKRA00004 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00014)

OKRA00014 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00015)

OKRA00015 1 each
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00016)

OKRA00016 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00017)

OKRA00017 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

AgileBlotter? Automated Western Blot Processor

ACT-ABH-40 each
EUR 20326.8

Rat Ceruloplasmin protein control for western blot

CP17-C 100 ul
EUR 343.2

Rat Normal Tissue Blot I

1581 One blot
EUR 493.8
Description: The rat tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The rat tissues included in the blot are brain, colon, heart, kidney, liver, lung, pancreas, and spleen.

Rat Normal Tissue Blot II

1582 One blot
EUR 493.8
Description: The rat tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The rat tissues included in the blot are adrenal, cerebellum, cerebrum, small intestine, skeletal muscle, stomach, testis, and thymus.

Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) IV

T8234601 5 slides
EUR 313.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) V

T8234602 5 slides
EUR 313.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) VI

T8234603 5 slides
EUR 313.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) VII

T8234604 5 slides
EUR 313.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) VIII

T8234605 5 slides
EUR 298.8
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) IX

T8234606 5 slides
EUR 298.8
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) X

T8234607 5 slides
EUR 298.8
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

ChonBlock Western Blot Buffer 2 for Goat (PBS)

90691P 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Goat (PBS)

ChonBlock Western Blot Buffer 2 for Goat (TBS)

90691T 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Goat (TBS)

ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

90692P 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

90692T 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

Human Leptin protein Western blot +ve control # 2

LEP12-C 100 ul
EUR 343.2

PathwayReady? JAK/STAT Signaling Inhibitor Panel

K864-8 each
EUR 1136.4

Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel I, 8 tissue spots

T8235437 5 slides
EUR 651.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel II, 8 tissue spots

T8235438 5 slides
EUR 651.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel III, 8 tissue spots

T8235439 5 slides
EUR 651.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel IV, 8 tissue spots

T8235440 5 slides
EUR 651.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel V, 8 tissue spots

T8235441 5 slides
EUR 651.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel VI, 8 tissue spots

T8235442 5 slides
EUR 651.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel VII, 8 tissue spots

T8235443 5 slides
EUR 651.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

ChonBlock Western Blot Buffer 1 (PBS)

9069P 10 ml
EUR 232.26
Description: ChonBlock Western Blot Buffer 1 (PBS)

ChonBlock Western Blot Buffer 1 (TBS)

9069T 10 ml
EUR 232.26
Description: ChonBlock Western Blot Buffer 1 (TBS)

Western Blot Primary Antibody Diluent Buffer

abx090689-100ml 100 ml
EUR 218.4

cyclin G2 Western Blot kit (AWBK03032)

AWBK03032 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

Attoglow Western Blot Analysis Kit:Blocking Agent

K3171250-3 40 g
EUR 130.8
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

T-Pro Western Blot Stripping Reagent

JB11-K002 500ml/BT
EUR 213.6

Pure-IP Western Blot Detection Kit

PRB-5002 20 blots
EUR 470.4
Description: Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection Kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody.

Frozen Tissue Section Panel - Rat Normal Tissue, Multi-tissue III

T6434423 5 slides
EUR 588
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Frozen Tissue Section Panel - Rat Normal Tissue, Multi-tissue I

T6434448 5 slides
EUR 588
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Rat Normal Tissue, Multi-tissue III

T8434423 5 slides
EUR 313.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Rat Normal Tissue, Multi-tissue I

T8434448 5 slides
EUR 313.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Rat Normal Tissue, Multi-tissue II

T8434608 5 slides
EUR 313.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Purified Ram Cyclooxygenase (Cox-2) Western blot +ve control

COX21-C 100 ul
EUR 343.2

Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) I: Major Organ

T8234431 5 slides
EUR 313.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) II: Major Organ

T8234432 5 slides
EUR 313.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) III: Major Organ

T8234433 5 slides
EUR 313.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Monkey (Cynomolgus) Normal Tissue, Multi-tissue III, 8 different tissues

T8534423-Cy 5 slides
EUR 313.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Monkey (Cynomolgus) Normal Tissue, Multi-tissue I, 8 different tissues

T8534448-Cy 5 slides
EUR 313.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Total Protein Western Blots - Human Adult Normal Tissue, Blot IV, 16 lanes

W1234404 1
EUR 1120.8
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Purified rat serum albumin protein control for Western blot

ALBR12-C 100 ul
EUR 343.2

Western blot Kit for Rat Primary Antibodies, Chemilum. Substrate

80203-Rt 1 kit
EUR 781.2

Mouse Ceruloplasmin protein control for western blot

CP16-C 100 ul
EUR 343.2

Poly-ADP-ribosylated proteins for Western blot

ADPR11-C 100 ul
EUR 343.2

Recombinant Human MutY Protein for Western blot

MUTY11-C 100 ul
EUR 343.2

Nitrated Mol Wt Std for Western blot

NITT12-C 100 ul
EUR 343.2

Attoglow Western Blot Analysis Kit: Millennium Enhancer

K3171250-1 100 ml
EUR 195.6
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Box Western Blot Polystyrene Transparent 95x30x16mm X5

DD62676 PK5
EUR 94.62

SuperBrite? ECL Western Blot Substrate/Detection Kit

K824-50 each
EUR 229.2

1730 8 SNAP-SEAL 8 OZ

1730-8 100/pk
EUR 88.8
Description: Disposable Plastic; Plastic Containers

Frozen Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue III, 8 different tissues

T6236446Alz 5 slides
EUR 1249.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue I, 8 different tissues

T8236444Alz 5 slides
EUR 738
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue III, 8 different tissues

T8236446Alz 5 slides
EUR 738
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Recombinant Porcine Circovirus 2 replicase protein Control for Western Blot

PCV2R11-C 100 ul
EUR 343.2

Human Normal Tissue Blot II

1522 One blot
EUR 544.2
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, heart, kidney, liver, lung, pancreas, skeletal muscle, skin, and spleen.

Human Normal Tissue Blot VI

1526 One blot
EUR 544.2
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The human normal tissues included in the blot are left cerebellum, right cerebellum, frontal lobe, occipital lobe, parietal lobe, whole eye, spinal cord, temporal lobe, and thalamus.

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