Protein Quantification Utilizing the “Fast WesternBlot” Strategy
For the quantification of sure proteins of curiosity inside a fancy pattern, Western blot evaluation is probably the most broadly used methodology. It allows detection of a goal protein based mostly on using particular antibodies. Nevertheless, the entire process is commonly very time-consuming. However, with the event of quick blotting techniques and additional growth of immunostaining strategies, a discount of the processing time may be achieved.
Main challenges for the dependable protein quantification by Western blotting are satisfactory knowledge normalization and secure protein detection. Normally, normalization of the goal protein sign is carried out based mostly on housekeeping proteins (e.g., glyceraldehyde 3-phosphate dehydrogenase, ß-actin) with the belief that these proteins are expressed constitutively on the similar stage throughout experiments. Nevertheless, a number of research have already proven that this isn’t all the time the case making this method suboptimal.
One other technique makes use of complete protein normalization the place the abundance of the goal protein is expounded to the entire protein quantity in every lane. This method is impartial of a single loading management, and precision of quantification and reliability is elevated. For Western blotting a number of detection strategies can be found, e.g., colorimetric, chemiluminescent, radioactive, fluorescent detection. Standard colorimetric staining tends to endure from low sensitivity, restricted dynamic vary, and low reproducibility. Chemiluminescence-based strategies are easy, however the detected sign doesn’t linearly correlate to protein abundance (from protein quantities >5μg) and have a comparatively slim dynamic vary. Radioactivity is dangerous to well being.
To beat these limitations, stain-free strategies had been developed permitting the mixture of fluorescent requirements and a stain-free fluorescence-based visualization of complete protein in gels and after switch to the membrane. Right here, we current a speedy Western blot protocol, which mixes quick blotting utilizing the iBlot system and quick immunostaining using ReadyTector®all-in-one resolution with the Sensible Protein Layers (SPL) method.
Close to misdiagnosis of acute HIV-infection with ELISA-WesternBlot scheme: Time for mindset change
Some HIV-infection diagnostic tips and well being care suppliers nonetheless depend on the ELISA-Western blot diagnostic algorithm. We current a close to misdiagnosis case with discordant check outcomes and a scarcity of correct counseling. We level out the necessity for an assertive replace of well being care suppliers on diagnostic HIV-tests.
Preclinical WesternBlot within the Period of Digital Transformation and Reproducible Analysis, an Jap Perspective
The present analysis is an interdisciplinary endeavor to develop a essential software in preclinical protein research of illnesses or problems via western blotting. Within the period of digital transformation and open entry rules, an interactive cloud-based database referred to as East-West Blot ( https://rancs-lab.shinyapps.io/WesternBlots ) is designed and developed. The net interactive subject-specific database constructed on the R shiny platform facilitates a scientific literature search on the precise subject material, right here set to western blot research of protein regulation within the preclinical mannequin of TBI.
The software summarizes the present publicly obtainable information via an information visualization approach and quick access to the essential knowledge components and hyperlinks to the examine itself. The applying compiled a relational database of PubMed-indexed western blot research labeled beneath HHS public entry, reporting downstream protein rules offered by fluid percussion damage mannequin of traumatic mind damage.
The guarantees of the developed software embrace progressing towards implementing the rules of 3Rs (alternative, discount, and refinement) for humane experiments, cultivating the conditions of reproducible analysis when it comes to reporting traits, paving the methods for a extra collaborative experimental design in fundamental science, and rendering an up-to-date and summarized perspective of present publicly obtainable information.
nwreprosci
Antigen-Antibody Pen For Chicken Primary antibodies
Description: Our Enhanced Chemiluminescent Kit is an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Powered by our proprietary and easy to use substrates, a unique blocking agent, highly specific secondary antibodies, and carefully optimized protocols, this kit offers the highest signal to noise ratio available and yields clean results, blot after blot.
Description: Our Enhanced Chemiluminescent Kit is an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Powered by our proprietary and easy to use substrates, a unique blocking agent, highly specific secondary antibodies, and carefully optimized protocols, this kit offers the highest signal to noise ratio available and yields clean results, blot after blot.
Description: Our Enhanced Chemiluminescent Kit is an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Powered by our proprietary and easy to use substrates, a unique blocking agent, highly specific secondary antibodies, and carefully optimized protocols, this kit offers the highest signal to noise ratio available and yields clean results, blot after blot.
Description: Our Enhanced Chemiluminescent Kit is an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Powered by our proprietary and easy to use substrates, a unique blocking agent, highly specific secondary antibodies, and carefully optimized protocols, this kit offers the highest signal to noise ratio available and yields clean results, blot after blot.
Description: Our Enhanced Chemiluminescent Kit is an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Powered by our proprietary and easy to use substrates, a unique blocking agent, highly specific secondary antibodies, and carefully optimized protocols, this kit offers the highest signal to noise ratio available and yields clean results, blot after blot.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PSENEN / PEN-2 (N-Terminus). This antibody is tested and proven to work in the following applications:
Description: DAB is the chromogenic substrate of peroxidase. The reaction product is a brown precipitate insoluble in water, dimethylbenzene or alcohol, which makes DAB suitable for color development reaction in western blotting. This Western Blotting DAB Chromogenic kit provides an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Our proprietary polymeric labeling of peroxidase conjugated secondary antibodies offers superior sensitivity in western blotting, significant increase in the detection range, high signalâ€toâ€noise ratio, and lower primary antibodies consumption. Powered by our proprietary polymeric labeling technique, DAB substrates, a unique blocking agent, highly specific secondary antibodies and carefully optimized protocols, this kit yields cleanest results, blot after blot.
Description: DAB is the chromogenic substrate of peroxidase. The reaction product is a brown precipitate insoluble in water, dimethylbenzene or alcohol, which makes DAB suitable for color development reaction in western blotting. This Western Blotting DAB Chromogenic kit provides an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Our proprietary polymeric labeling of peroxidase conjugated secondary antibodies offers superior sensitivity in western blotting, significant increase in the detection range, high signalâ€toâ€noise ratio, and lower primary antibodies consumption. Powered by our proprietary polymeric labeling technique, DAB substrates, a unique blocking agent, highly specific secondary antibodies and carefully optimized protocols, this kit yields cleanest results, blot after blot.
Description: DAB is the chromogenic substrate of peroxidase. The reaction product is a brown precipitate insoluble in water, dimethylbenzene or alcohol, which makes DAB suitable for color development reaction in western blotting. This Western Blotting DAB Chromogenic kit provides an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Our proprietary polymeric labeling of peroxidase conjugated secondary antibodies offers superior sensitivity in western blotting, significant increase in the detection range, high signalâ€toâ€noise ratio, and lower primary antibodies consumption. Powered by our proprietary polymeric labeling technique, DAB substrates, a unique blocking agent, highly specific secondary antibodies and carefully optimized protocols, this kit yields cleanest results, blot after blot.
Description: DAB is the chromogenic substrate of peroxidase. The reaction product is a brown precipitate insoluble in water, dimethylbenzene or alcohol, which makes DAB suitable for color development reaction in western blotting. This Western Blotting DAB Chromogenic kit provides an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Our proprietary polymeric labeling of peroxidase conjugated secondary antibodies offers superior sensitivity in western blotting, significant increase in the detection range, high signalâ€toâ€noise ratio, and lower primary antibodies consumption. Powered by our proprietary polymeric labeling technique, DAB substrates, a unique blocking agent, highly specific secondary antibodies and carefully optimized protocols, this kit yields cleanest results, blot after blot.
Description: DAB is the chromogenic substrate of peroxidase. The reaction product is a brown precipitate insoluble in water, dimethylbenzene or alcohol, which makes DAB suitable for color development reaction in western blotting. This Western Blotting DAB Chromogenic kit provides an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Our proprietary polymeric labeling of peroxidase conjugated secondary antibodies offers superior sensitivity in western blotting, significant increase in the detection range, high signalâ€toâ€noise ratio, and lower primary antibodies consumption. Powered by our proprietary polymeric labeling technique, DAB substrates, a unique blocking agent, highly specific secondary antibodies and carefully optimized protocols, this kit yields cleanest results, blot after blot.
Description: DAB is the chromogenic substrate of peroxidase. The reaction product is a brown precipitate insoluble in water, dimethylbenzene or alcohol, which makes DAB suitable for color development reaction in western blotting. This Western Blotting DAB Chromogenic kit provides an easy, consistent, sensitive and highly specific detection solution to your protein of interest. Our proprietary polymeric labeling of peroxidase conjugated secondary antibodies offers superior sensitivity in western blotting, significant increase in the detection range, high signalâ€toâ€noise ratio, and lower primary antibodies consumption. Powered by our proprietary polymeric labeling technique, DAB substrates, a unique blocking agent, highly specific secondary antibodies and carefully optimized protocols, this kit yields cleanest results, blot after blot.
Apoptosis is a sort of programmed cell loss of life induced by a cascade of biochemical occasions, which ends up in distinct morphological adjustments characterised by cell shrinkage, membrane blebbing, chromatin condensation, and DNA fragmentation. Apoptosis is executed by a category of cysteine proteases referred to as caspases. Caspases are synthesized as inactive pro-caspases and activated by a sequence of cleavage reactions. Lively caspases cleave mobile substrates and are thus the principle effectors of the apoptotic cell loss of life pathway. Detection of caspase cleavage by western blot evaluation is a standard methodology to exhibit the induction of apoptosis.
Within the context of apoptosis, the right evaluation of western blot outcomes relies on the understanding of the mechanisms and outcomes of caspase processing throughout the course of its activation. On this chapter, we describe the step-by-step methodology within the western blot evaluation of caspase cleavage throughout apoptosis. We element protocols for protein extraction, quantitation, casting, and working gel electrophoresis and western blot evaluation of caspase -Eight and caspase -9 activation. The described strategies may be utilized to any explicit protein of curiosity.
Spreader-CDR system for environment friendly, reproducible and frugal westernblot
Environment friendly antibody incubation is an important step for profitable western blot. Throughout the incubation, a skinny antibody-depleted layer is created across the blotting membrane which limits antibody binding. Though the traditional batch shaking methodology is ineffective towards it, this layer may be simply disrupted by cyclic draining and replenishing (CDR) of the antibody resolution throughout membrane incubation. Beforehand, we launched a closed and rotating cylindrical chamber as a software to implement CDR for western blots (rCDR). A brand new open bucket-style chamber was devised for simpler operation and the potential of course of automation.
As an alternative of rotation as in rCDR, rocking it backwards and forwards achieved the CDR antibody incubation (R-CDR). The chamber was then geared up with a spreader-rod to facilitate the uniform motion of the antibody resolution throughout the membrane floor. Therefore it was named spreader CDR (S-CDR). In comparison with the batch incubation methodology, each the S-CDR and R-CDR gadgets produced considerably enhanced alerts and developed quicker outcomes. There have been a number of further advantages of utilizing the spreader-rod, which included uniform antibody binding throughout the membrane, diminished utilization of antibodies and the power to recuperate outcomes even from mishandled, creased membranes. The S-CDR machine ensures higher blots and may be simply applied in current western blot protocols. This text is protected by copyright. All rights reserved.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: The rat tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The rat tissues included in the blot are brain, colon, heart, kidney, liver, lung, pancreas, and spleen.
Description: The rat tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The rat tissues included in the blot are adrenal, cerebellum, cerebrum, small intestine, skeletal muscle, stomach, testis, and thymus.
Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) IV
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) V
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) VI
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) VII
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) VIII
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) IX
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) X
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel II, 8 tissue spots
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel III, 8 tissue spots
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel IV, 8 tissue spots
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel V, 8 tissue spots
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel VI, 8 tissue spots
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel VII, 8 tissue spots
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection Kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody.
Frozen Tissue Section Panel - Rat Normal Tissue, Multi-tissue III
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Frozen Tissue Section Panel - Rat Normal Tissue, Multi-tissue I
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Rat Normal Tissue, Multi-tissue III
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Rat Normal Tissue, Multi-tissue I
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Rat Normal Tissue, Multi-tissue II
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Purified Ram Cyclooxygenase (Cox-2) Western blot +ve control
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) II: Major Organ
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) III: Major Organ
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Monkey (Cynomolgus) Normal Tissue, Multi-tissue III, 8 different tissues
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Monkey (Cynomolgus) Normal Tissue, Multi-tissue I, 8 different tissues
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Total Protein Western Blots - Human Adult Normal Tissue, Blot IV, 16 lanes
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Purified rat serum albumin protein control for Western blot
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Box Western Blot Polystyrene Transparent 95x30x16mm X5
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue I, 8 different tissues
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue III, 8 different tissues
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Recombinant Porcine Circovirus 2 replicase protein Control for Western Blot
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, heart, kidney, liver, lung, pancreas, skeletal muscle, skin, and spleen.
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The human normal tissues included in the blot are left cerebellum, right cerebellum, frontal lobe, occipital lobe, parietal lobe, whole eye, spinal cord, temporal lobe, and thalamus.
